Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Bagavan, Asokan; Rahuman, Abdul Abdul; Kamaraj, Chinnaperumal; Kaushik, Neeraj; Mohanakrishnan, Dinesh; Sahal, Dinkar

doi:10.1007/s00436-010-2151-0

Cited by 61 publications

(42 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Today numerous plant materials are available, which have shown promising first results in the search for new bioactive compounds against Plasmodium spp. (Bagavan et al 2011a;Bagavan et al 2011b). Furthermore, interesting activities of Brazilian indigenous plants (Brandelli et al 2009) and phenol-rich essential oils (Machado et al 2010) were found against Giardia lamblia and various plants had amoebicidal activity against Acanthamoeba spp.…”

Section: Discussionmentioning

confidence: 99%

In vitro determination of anticryptosporidial activity of phytogenic extracts and compounds

et al. 2012

View full text Add to dashboard Cite

Cryptosporidiosis caused by Cryptosporidium spp. is an important diarrhoeal disease observed in farm animals and humans, especially in young or immunocompromised individuals. A novel cell culture assay for testing extracts and pure compounds against Cryptosporidium parvum in 96-well microplate format was established and evaluated. It is based on previously described indirect fluorescent antibody techniques and was optimised for higher sample throughput. Rapid assessment of minimal inhibitory concentrations (MICs) was done by checking each well microscopically for the presence or absence of parasite stages. As a novelty, parasite development was quantified by enumeration of clusters of secondary infection (CSI), which typically appeared upon infection with a distinct parasite inoculum after a defined incubation time. Host cell (HCT-8) viability was measured by an integrated non-destructive water-soluble tetrazolium salt assay (WST-1), which facilitated discrimination of antiparasitic activity from possible cytotoxic effects of a test compound against the host cells. Host cell viability was regarded unimpaired when cultures had 75% or more viability when compared to control cultures without test substance. In this study, a maximum density of distinguishable CSI was obtained when cultures were infected with 2.5 × 10(3) oocysts and incubated for 48 h. The applicable inoculum has to be optimised for each batch of oocysts and before each experimental series. Parasite development was inhibited completely by monensin at 134 nM and silymarin at 50 mg/mL. These concentrations were non-toxic to the host cells and comparable to literature data. The percentages of parasite inhibition were determined for monensin and a 50% inhibitory concentration (IC(50)) of 36.6 nM (27.4-45.5) and a 90% inhibitory concentration of 65.9 nM (54.8-90.2) were calculated. The introduced assay is economic because relatively low parasite numbers may be used. If MICs are determined, evaluation is fast, as each well is viewed only briefly under the fluorescence microscope for presence or absence of CSI. Furthermore it is highly critical because only full parasite inhibition is assessed. Counting of CSI is more laborious and time-consuming, but it allows calculation of parasite inhibition rates and parameters like the half maximal inhibitory concentration (IC(50)). This assay shall be used to assess anticryptosporidial activities of various plant waste materials and by-products from the food and the pharmaceutical industries in the course of the EU project SAFEWASTES. Comparison with in vivo models should be performed to further corroborate the results. Automated evaluation by flow cytometry might facilitate higher sample throughput and reduce operator bias.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro determination of anticryptosporidial activity of phytogenic extracts and compounds

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Phyllanthus urinaria was mentioned in this study as being used to manage and treat high blood pressure, malaria, sore throat, and ulcer. The antiplasmodial activity of Phyllanthus urinaria has been well known [136,137]. Hippomanin A and corilagin isolated from P. urinaria were found with in vitro antiviral activity [138].…”

Section: Potential Allelopathic Effects Of Volatile Constituents Frommentioning

confidence: 99%

Exploring Alternative Use of Medicinal Plants for Sustainable Weed Management

et al. 2017

View full text Add to dashboard Cite

This paper presents the first application of ethnobotanical studies to screen for allelopathic species among medicinal plants for sustainable weed management. This study assesses the possible relationship between ethnobotanical indices and allelopathy of medicinal plants. Ethnobotanical data were collected in 2016 by using semi-structured interviews with 140 informants in the Ejisu-Juaben Municipality, Ghana. Data were analysed using statistical tool and ethnobotanical indices including use value (UV), Fidelity Level (FL), Relative Frequency of Citation (RFC). The Sandwich and Dish pack methods were respectively used to evaluate allelopathy through leachates and volatiles of collected samples. Ninety-five species belonging to 43 families are reported in this study, with leaves (52%) cited the most utilised plant part. Cleistopholis patens (UV = 0.54; FL = 90.7%; RFC = 0.37) and Ocimum gratissimum (UV = 0.37; FL = 38.4%; RFC = 0.35) were among the most cited species. Thirty-two species showed inhibition (≥49.3%) by leachates, while twenty-four species were found with potential volatile inhibitory compounds against lettuce radicle growth. There was a significant positive correlation (Pearson) between the UV and RFC of medicinal plants and allelopathy by leaf leachates (r = 0.639 **; p = 0.01 and r = 0.653 **; p = 0.01 respectively). This systematic documentation of medicinal plants in Ejisu-Juaben Municipality shows medicinal plants with ethnomedicinal values and potential allelopathy that can be utilised in sustainable weed control.

show abstract

“…The ethanol extract of A. aspera stem showed the IC 50 value of 153 μg/mL (Simonsen et al 2001). Bagavan et al (2011a) studied the anti-plasmodial activity of different solvent extracts of Citrus sinensis (peel), Leucas aspera, O. sanctum, Phyllanthus acidus (leaf) and Terminalia chebula (seed) against P. falciparum. Good antiplasmodial activity has been reported in the leaf ethyl acetate and methanol extracts of L. aspera, ethyl acetate, acetone and methanol extracts of P. acidus and seed acetone extract of T. chebula (IC 50 07.…”

Section: Resultsmentioning

confidence: 99%

In vitro anti-plasmodial activity of some traditionally used medicinal plants against Plasmodium falciparum

et al. 2012

View full text Add to dashboard Cite

The anti-plasmodial activity of different solvent extracts of Adhatoda vasica (root), Caesalpinia pulcherrima (leaf), Carica papaya (pulp), Erythroxylum monogynum (leaf), Lantana camara (whole plant), Ocimum sanctum (root) and Phyllanthus niruri (whole plant) were studied against Plasmodium falciparum. Of the 35 extracts tested, seven extracts showed good anti-plasmodial activity. Methanol extract of C. pulcherrima showed the lowest IC50 value (10.96 μg/mL) followed by methanol extract of A. vasica (IC(50)=11.1 μg/mL), chloroform extract of O. sanctum (IC(50)=11.47 μg/mL), methanol extract of E. monogynum (IC(50)=12.23 μg/mL), acetone extract of C. pulcherrima (IC(50)=12.49 μg/mL), methanol extract of O. sanctum and acetone extract of A. vasica (IC(50)=14.04 μg/mL). The results of the present study justify the use of these medicinal plants in traditional practice, and also, a further study on the isolation of anti-plasmodial molecules from their active crude extracts is in progress.

show abstract

Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Cited by 61 publications

References 71 publications

In vitro determination of anticryptosporidial activity of phytogenic extracts and compounds

In vitro determination of anticryptosporidial activity of phytogenic extracts and compounds

Exploring Alternative Use of Medicinal Plants for Sustainable Weed Management

In vitro anti-plasmodial activity of some traditionally used medicinal plants against Plasmodium falciparum

Contact Info

Product

Resources

About