Antiparasitic and physiological evaluation of Curcuma longa extract and/or PZQ on Schistosoma mansoni infected mice.

Abstract

Praziquantel (PZQ) is the drug of choice against schistosomiasis. However, PZQ was found not fully effective. A new trend from natural plant sources was used to compete schistosomiasis. In the present study, the antischistosomal activities of Curcumin extract and /or PZQ were evaluated. Parasitological and histopathological examination of liver has been explored. Moreover, Surface topography of the recovered worms was inspected by SEM. On the other hand, the total protein and DNA fragmentation percentage were estimated in infected and infected treated mice as compared to control group. Treatment of infected CD1 mice with curcumin extract alone didn't show any detectable changes in the parasitological or histological parameters. The infected mice treated with PZQ only, showed a significant decrease in the total worm burden, egg load (52%), with increase in degenerated eggs (58%) in the oogram pattern.However, treating infected CD1 mice with combined therapy (curcumin extract + PZQ) showed extremely significant reduction in female and male worm count (93% & 84%) respectively. Liver tissue egg load decreased by 88%, intestinal egg load decreased by 97%. Combined therapy decreased granuloma count and diameter with significant increase in degenerated egg in the oogram count. Moreover, the studied biomarkers, related to liver function and DNA damage were significantly improved in infected mice treated with curcumin combined with PZQ as compared to either drug alone. . In conclusion, curcumin extract in combination with PZQ has a synergetic effect and could be more promising in controlling schistosomiasis.Copy Right, IJAR, 2016,. All rights reserved.

Parasitological and Biochemical Efficacy of the Active Ingredients of Allium sativum and Curcuma longa in Schistosoma mansoni Infected Mice

“…The reduction rates of the worms were convergent in the PZQ and CU groups (86.02% and 86%, respectively). On the contrary, with the present result, C. longa extract alone showed a low reduction rate of the worms that didn’t exceed 35% [ 22 ]. This observation may improve the outbalance efficacy of the active ingredient of C. longa more than that of the plant itself.…”

“…The data of PZQ disagrees with Eid et al [ 38 ], who found that infected animals treated with PZQ showed no significant change in DNA fragmentation pattern in comparison with the infected-untreated group. However, the combination of PZQ and CU showed a marked enhancement of damaged DNA in infected animals [ 22 ].…”

“…Curcumin (CU), the active ingredient of Curcuma longa is a naturally basic phenolic compound produced as a yellow pigment from turmeric, which is generally used as a spice and food colorant [ 21 ]. Recently, it was reported that C. longa extract had an antischistosomal effect, which was proven by histological, physiological and molecular studies [ 22 ].…”

HPLC-ESI-MS Characterization of Certain Polyphenolic Compounds of Carica papaya L. Fruit Extracts and Evaluation of Their Potential Against Murine Schistosomiasis mansoni

“…The butanolic fraction group gave the strongest reduction in immature and mature ova (9.5 and 8.8 %, P < 0.001, respectively) and increased the percent of dead ova (81.7 %). The changes in the number and character of ova in the Oogram provide a sensitive, simple, and reliable criterion for the screening of the plant extracts against S. mansoni that represents the effects of the combined therapy on oviposition [22]. The results from this study were in agreement with previously reported studies that showed significant reductions in tissue egg load with the administration of pomegranate (Punica granatum) and onion (Allium cepa) extracts [23,24].…”