Antioxidative Properties of Functional Polyphenols and Their Metabolites Assessed by an ORAC Assay

Ishimoto, Hidekazu; Tai, Akihiro; Yoshimura, Manabu; Amakura, Yoshiaki; Yoshida, Takashi; Hatano, Tsutomu; Ito, Hideyuki

doi:10.1271/bbb.110717

Cited by 64 publications

(54 citation statements)

References 39 publications

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“…ORAC values were measured with fluorescence spectrometer (Victor 2 Wallac–Perkin‐Elmer) by inhibition of 2′,7′dichlorofluorescein (DCF; Ishimoto and others ) with slight modifications. Briefly, all samples and reagents were dissolved in 10 mmol phosphate L −1 /150 mmol NaCl L −1 buffer (PBS) at pH 7.4.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, the inhibition of lipid peroxidation during digestion is an important role of radical‐scavenging dietary antioxidants. It is now recognized that diets rich in antioxidants derived from fruits and vegetables are associated with lower risks of cardiovascular diseases and cancers (Ishimoto and others ). Thus, interest in naturally occurring antioxidants has considerably increased for use in food, cosmetic, and pharmaceutical products (Djeridane and others ).…”

Section: Introductionmentioning

confidence: 99%

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Antioxidative Properties and Ability of Phenolic Compounds of Myrtus communis Leaves to Counteract In Vitro LDL and Phospholipid Aqueous Dispersion Oxidation

Dairi

Madani

Aoun³

et al. 2014

Journal of Food Science

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antioxidative Properties and Ability of Phenolic Compounds of Myrtus communis Leaves to Counteract In Vitro LDL and Phospholipid Aqueous Dispersion Oxidation

Dairi

Madani

Aoun³

et al. 2014

Journal of Food Science

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“…However, using the ORAC assay, all urolithins tested exhibited potent antioxidant properties compared with those of ascorbic acid, urolithin A being the most potent among them [29]. In a similar study, in which the antioxidant activity of a large number of polyphenols and their metabolites produced in vivo was compared by the ORAC method, the antioxidant activity of urolithin A was one of the most powerful, only beaten by some proanthocyanidin oligomers, catechin, epicatechin and 3,4-dihydroxyphenyl acetic acid [52]. Using a cell-based method, in which the transport through the cell membrane was considered, Bialonska et al [39] determined that urolithin C showed the highest antioxidant power (IC 50 = 0.16 μ M) whereas urolithin A showed an IC 50 of 13.6 μ M, still in the range of the plasma concentrations achievable in vivo but in a lesser degree than its precursor ellagic acid (1.1 μ M) or vitamin C (1.9 μ M) [39].…”

Section: Biological Activity Of Urolithinsmentioning

confidence: 99%

Biological Significance of Urolithins, the Gut Microbial Ellagic Acid-Derived Metabolites: The Evidence So Far

Espı́n

Larrosa

Garcı́a-Conesa

et al. 2013

Evidence-Based Complementary and Alternative Medicine

450

430

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The health benefits attributed to pomegranate have been associated with its high content in polyphenols, particularly ellagitannins. This is also the case for other ellagitannin-containing fruits and nuts including strawberry, raspberry, blackberry, walnuts, and muscadine grapes. The bioavailability of ellagitannins and ellagic acid is however very low. These molecules suffer extensive metabolism by the gut microbiota to produce urolithins that are much better absorbed. Urolithins circulate in plasma as glucuronide and sulfate conjugates at concentrations in the range of 0.2–20 μM. It is therefore conceivable that the health effects of ellagitannin-containing products can be associated with these gut-produced urolithins, and thus the evaluation of the biological effects of these metabolites is essential. Recent research, mostly based on in vitro testing, has shown preliminary evidence of the anti-inflammatory, anticarcinogenic, antiglycative, antioxidant, and antimicrobial effects of urolithins, supporting their potential contribution to the health effects attributed to pomegranate and ellagitannin-rich foods. The number of in vivo studies is still limited, but they show preventive effects of urolithins on gut and systemic inflammation that encourage further research. Both in vivo and mechanistic studies are necessary to clarify the health effects of these metabolites. Attention should be paid when designing these mechanistic studies in order to use the physiologically relevant metabolites (urolithins in gut models and their conjugated derivatives in systemic models) at concentrations that can be reached in vivo.

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“…Similarly to other polyphenols in the human diet, chlorogenic acids have important protective antioxidant properties. Indeed, coffee is the major daily dietary source of antioxidants in many countries 19,20 . It has been shown to perform this protective function in cortical neurons subjected to H 2 O 2 -induced apoptosis.…”

Section: Chlorogenic Acids (Cga)mentioning

confidence: 99%

Caffeine and Chlorogenic Acids in Coffee and Effects on Selected Neurodegenerative Diseases

Santos¹,

Hunter²,

Wright³

et al. 2013

J Pharm Sci Innov

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Much controversy and misunderstanding has surrounded the disputed health impact of coffee consumption. Recent studies now help clarify the pharmacological and neurological health promoting properties of coffee daily intake. Benefits are shown attributable to coffee's rich phytochemistry that includes (besides caffeine), phenolic compounds, such as chlorogenic acids, diterpenes, trigonelline, minerals, amino acids and other volatile compounds. It is also a powerful disease fighting antioxidant, the primary source of antioxidants in several countries. The purpose of this article is to review results from current epidemiological and mechanistic studies on the central nervous system attributed to coffee. A primary focus to this review is the activity of caffeine and chlorogenic acids and their implications to CNS health. The discussion will highlight possible molecular mechanisms responsible for the respective pharmacological benefits.

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Antioxidative Properties of Functional Polyphenols and Their Metabolites Assessed by an ORAC Assay

Cited by 64 publications

References 39 publications

Antioxidative Properties and Ability of Phenolic Compounds of Myrtus communis Leaves to Counteract In Vitro LDL and Phospholipid Aqueous Dispersion Oxidation

Antioxidative Properties and Ability of Phenolic Compounds of Myrtus communis Leaves to Counteract In Vitro LDL and Phospholipid Aqueous Dispersion Oxidation

Biological Significance of Urolithins, the Gut Microbial Ellagic Acid-Derived Metabolites: The Evidence So Far

Caffeine and Chlorogenic Acids in Coffee and Effects on Selected Neurodegenerative Diseases

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