Antioxidative enzymes in cultivars of pepper plants with different sensitivity to cadmium

León, Alberto Sanz de; Palma, José M.; Corpas, Francisco J.; Gómez, M. A.; Romero‐Puertas, María C.; Chatterjee, Debasmita; Mateos, Rosa M.; Rı́o, Luis A. del; Sandalio, Luisa M.

doi:10.1016/s0981-9428(02)01444-4

Cited by 164 publications

(87 citation statements)

References 37 publications

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“…Our results agree with those of Sharma and Dubey [59], who observed increases in GR activity for Oryza sativa seedlings subjected to drought stress. Increases in GR activity under stresses of salt, Cd and Cu +2 were also observed in Capsicum annuum and C. orientinum [60,61]. The enhanced activity of GR might assist the regeneration of NADPH to NADP, thereby ensuring the availability of NADP to accept electrons from the photosynthetic electron transport chain [62].…”

Section: Discussionmentioning

confidence: 99%

Comparison of ROS formation and antioxidant enzymes in Cleome gynandra (C4) and Cleome spinosa (C3) under drought stress

et al. 2012

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a b s t r a c tDifferences between antioxidant responses to drought in C 3 and C 4 plants are rather scanty. Even, we are not aware of any research on comparative ROS formation and antioxidant enzymes in C 3 and C 4 species differing in carboxylation pathway of same genus which would be useful to prevent other differences in plant metabolism. With this aim, relative shoot growth rate, relative water content and osmotic potential, hydrogen peroxide (H 2 O 2 ) content and NADPH oxidase (NOX) activity, antioxidant defence system (superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR) enzymes and their isoenzymes), CAT1 mRNA level, and lipid peroxidation in seedlings of Cleome spinosa (C 3 ) and Cleome gynandra (C 4 ) species of Cleome genus exposed to drought stress for 5 and 10 day (d) were comparatively investigated. Constitutive levels of antioxidant enzymes (except SOD) were consistently higher in C. spinosa than in C. gynandra under control conditions. CAT1 gene expression in C. spinosa was correlated with CAT activity but CAT1 gene expression in C. gynandra at 10 d did not show this correlation. Drought stress caused an increase in POX, CAT, APX and GR in both species. However, SOD activity was slightly decreased in C. gynandra while it was remained unchanged or increased on 5 and 10 d of stress in C. spinosa, respectively. Parallel to results of malon dialdehyde (MDA), H 2 O 2 content was also remarkably increased in C. spinosa as compared to C. gynandra under drought stress. These results suggest that in C. spinosa, antioxidant defence system was insufficient to suppress the increasing ROS production under stress condition. On the other hand, in C. gynandra, although its induction was lower as compared to C. spinosa, antioxidant system was able to cope with ROS formation under drought stress.

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Section: Discussionmentioning

confidence: 99%

Comparison of ROS formation and antioxidant enzymes in Cleome gynandra (C4) and Cleome spinosa (C3) under drought stress

et al. 2012

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“…The finding of decreases in calcium, iron, manganese, and zinc in cadmium-treated P. 3 canescens is consistent with previous studies Metwally et al, 2005;Solti et al, 2008;Rodríguez-Serrano et al, 2009) and probably due to competition with cadmium ions for transporters. Reduction in CO 2 assimilation is probably due to defects in the photosynthetic apparatus (McCarthy et al, 2001;Solti et al, 2008;Ivanova et al, 2011), inactivation of photosynthesis-related enzymes (Leon et al, 2002;He et al, 2011), decreases in photosynthetic pigments or iron (Solti et al, 2008;Besson-Bard et al, 2009;Wu et al, 2012), and differential expression of photosynthesis-related genes and proteins (Kieffer et al, 2009a(Kieffer et al, , 2009bDurand et al, 2010). Reduced CO 2 assimilation often leads to changes in the concentrations of sugars and sugar alcohols in cadmium-treated plants (Devi et al, 2007;Kieffer et al, 2009a;He et al, 2011).…”

Section: Changed Nutrient and Carbohydrate Concentrations And Shiftedmentioning

confidence: 99%

A Transcriptomic Network Underlies Microstructural and Physiological Responses to Cadmium in Populus × canescens

et al. 2013

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Bark tissue of Populus 3 canescens can hyperaccumulate cadmium, but microstructural, transcriptomic, and physiological response mechanisms are poorly understood. Histochemical assays, transmission electron microscopic observations, energydispersive x-ray microanalysis, and transcriptomic and physiological analyses have been performed to enhance our understanding of cadmium accumulation and detoxification in P. 3 canescens. Cadmium was allocated to the phloem of the bark, and subcellular cadmium compartmentalization occurred mainly in vacuoles of phloem cells. Transcripts involved in microstructural alteration, changes in nutrition and primary metabolism, and stimulation of stress responses showed significantly differential expression in the bark of P. 3 canescens exposed to cadmium. About 48% of the differentially regulated transcripts formed a coregulation network in which 43 hub genes played a central role both in cross talk among distinct biological processes and in coordinating the transcriptomic regulation in the bark of P. 3 canescens in response to cadmium. The cadmium transcriptome in the bark of P. 3 canescens was mirrored by physiological readouts. Cadmium accumulation led to decreased total nitrogen, phosphorus, and calcium and increased sulfur in the bark. Cadmium inhibited photosynthesis, resulting in decreased carbohydrate levels. Cadmium induced oxidative stress and antioxidants, including free proline, soluble phenolics, ascorbate, and thiol compounds. These results suggest that orchestrated microstructural, transcriptomic, and physiological regulation may sustain cadmium hyperaccumulation in P. 3 canescens bark and provide new insights into engineering woody plants for phytoremediation.

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“…Millar at al., (2003) identified a family of seven related proteins in the cytosol, chloroplasts, mitochondria and endoplasmatic reticulum in Arabidopsis. Activity of GPX decreased in roots and did not change significantly in the leaves of Pisum sativum exposed on Cd-stress (Dixit et al, 2001), whereas activity of this enzyme increased in cultivars of Capsicum annuum under Cd-stress (Leon et al, 2002).…”

Section: Enzymatic Antioxidantsmentioning

confidence: 81%

ROS as Signaling Molecules and Enzymes of Plant Response to Unfavorable Environmental Conditions

Boguszewska¹,

Zagdańska²

2012

Oxidative Stress - Molecular Mechanisms and Biological Effects

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Antioxidative enzymes in cultivars of pepper plants with different sensitivity to cadmium

Cited by 164 publications

References 37 publications

Comparison of ROS formation and antioxidant enzymes in Cleome gynandra (C4) and Cleome spinosa (C3) under drought stress

Comparison of ROS formation and antioxidant enzymes in Cleome gynandra (C4) and Cleome spinosa (C3) under drought stress

A Transcriptomic Network Underlies Microstructural and Physiological Responses to Cadmium in Populus × canescens

ROS as Signaling Molecules and Enzymes of Plant Response to Unfavorable Environmental Conditions

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