Antioxidative, anticancer and genotoxic properties of α-pinene on N2a neuroblastoma cells

Aydın, Elanur; Türkez, Hasan; Geyikoğlu, Fatime

doi:10.2478/s11756-013-0230-2

Cited by 81 publications

(47 citation statements)

References 55 publications

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“…In addition to oxidative stress, previous studies reported that different mechanisms have been linked to plant products' cytotoxicity, including: (i) proteasome inhibition; (ii) topoisomerase inhibition; (iii) inhibition of fatty acid synthesis; (iv) accumulation of p53; (v) induction of cell cycle arrest; (vi) inhibition of phosphatidyl-inositol 3-kinase; or (vii) enhanced expression of c-fos and c-myc (Brusselmans et al, 2005). Aydin et al (2013) indicated that α-pinene is neither genotoxic nor mutagenic on healthy neurons or N2a NB cells and demonstrates that pure α-pinene possesses weak antioxidant and cytotoxic activity in cultured primary rat neurons. In addition, pure α-pinene has weak antioxidant properties and little anticancer potential on rat N2a NB cell line and suggests that α-pinene is of a limited therapeutic use as an anticancer agent.…”

Section: In Vitro Antiproliferative and Cytotoxic Activitymentioning

confidence: 99%

“…The observed antioxidant potential should be attributed to the phenolic oil constituents, while the oil chemoprotective efficacy against oxidative stress-mediated disorders is mainly due to its free radical scavenging properties. Recent studies have demonstrated that α-pinene (2,6,6-tri-methyl-bicyclo [3.1.1] hept-2-ene), the main component in Egyptian juniper, is present naturally in the essential oils of many aromatic plants and has antioxidant properties according to DPPH radical, hydroxyl radical, superoxide anion, malonaldehyde and β-carotene bleaching methods (Aydin et al, 2013). α-pinene was reported tohave a broad spectrum of biological activities, i.e.…”

Section: Antioxidative Activitymentioning

confidence: 99%

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Essential oils from Egyptian aromatic plants as antioxidant and novel anticancer agents in human cancer cell lines

Ramadan¹,

Ali²,

Ghanem³

et al. 2015

Grasas y Aceites

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SUMMARY:Inhibitors of tumor growth using extracts from aromatic plants are rapidly emerging as important new drug candidates for cancer therapy. The cytotoxicity and in vitro anticancer evaluation of the essential oils from thyme, juniper and clove has been assessed against five different human cancer cell lines (liver HepG2, breast MCF-7, prostate PC3, colon HCT116 and lung A549). A GC/MS analysis revealed that α-pinene, thymol and eugenol are the major components of Egyptian juniper, thyme and clove oils with concentrations of 31.19%, 79.15% and 82.71%, respectively. Strong antioxidant profiles of all the oils are revealed in vitro by DPPH and β-carotene bleaching assays. The results showed that clove oil was similarly potent to the reference drug, doxorubicin in prostate, colon and lung cell lines. Thyme oil was more effective than the doxorubicin in breast and lung cell lines while juniper oil was more effective than the doxorubicin in all the tested cancer cell lines except prostate cancer. In conclusion, the essential oils from Egyptian aromatic plants can be used as good candidates for novel therapeutic strategies for cancer as they possess significant anticancer activity. KEYWORDS:Anticancer; Antioxidant; Clove Juniper; Thyme RESUMEN: Aceites esenciales de plantas aromáticas egipcias como novedosos agentes anticancerígenos y antioxidantes en líneas celulares de cáncer humano. Los inhibidores de crecimiento de tumores usando extractos de plantas aromáticas están emergiendo con rapidez como nuevos e importantes medicamentos para el tratamiento del cáncer. La citotoxicidad y la acción anticancerígena in vitro de aceites esenciales de tomillo, enebro y clavo han sido evaluadas en cinco líneas celulares de cáncer humano (hígado HepG2, mama MCF-7, próstata PC3, colon HCT116 y pulmón A549). Los análisis de GC/MS mostraron que α-pineno, timol y eugenol son los principales componentes de los aceites egipcios de enebro, tomillo y clavo, con concentraciones de 31,19%, 79,15% y 82,71%, respectivamente. Se demuestra, mediante ensayos in vitro de blanqueo de DPPH y β-caroteno, el enérgico perfil antioxidante de todos los aceites. Los resultados mostraron que el aceite de clavo fue similar de potente al fármaco de referencia, doxorrubicina en las líneas celulares de próstata, colon y pulmón. El aceite de tomillo fue más efectivo que la doxorrubicina en las líneas celulares de mama y de pulmón, mientras que el aceite de enebro fue más eficaz que la doxorrubicina en todas las líneas celulares de cáncer ensayados, excepto en la de cáncer de próstata. En conclusión, los aceites esenciales de plantas aromáticas egipcias se pueden utilizar como buenos candidatos para nuevas estrategias terapéuticas para el cáncer al poseer una significativa actividad anticancerígena

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Section: In Vitro Antiproliferative and Cytotoxic Activitymentioning

confidence: 99%

Section: Antioxidative Activitymentioning

confidence: 99%

Essential oils from Egyptian aromatic plants as antioxidant and novel anticancer agents in human cancer cell lines

Ramadan¹,

Ali²,

Ghanem³

et al. 2015

Grasas y Aceites

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show abstract

“…At a low exposure level, α-pinene is an effective bronchodilator and can be used for asthma treatment. Experimental studies also indicate that it exhibits antioxidant and antimicrobial activities [6,7]. It exhibits anticancer activities via distinct molecular mechanisms, i.e., the regulation of cancer cell cycle regulation, antioxidation, the reduction of inflammation, and the induction of cell apoptosis [8,9,10].…”

Section: Introductionmentioning

confidence: 99%

α-Pinene Inhibits Human Prostate Cancer Growth in a Mouse Xenograft Model

et al. 2017

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Background: α-Pinene is one of the most widely found terpenoids in nature. Substantial evidence shows that α-pinene has cancer prevention properties. In this study, the PC-3 cell line was used to establish subcutaneous xenograft tumors in nude mice. Methods: Cytotoxicity was measured with the MTT assay, and apoptosis and cell cycle analyses were conducted using flow cytometry in vitro. The PC-3 cell line was used to establish subcutaneous xenograft tumors in nude mice. Results: We found that treatment with α-pinene significantly inhibited human prostate cancer cell growth and induced apoptosis and cell cycle arrest in the cell line-based model. Furthermore, tumor progression was inhibited more in mice treated with α-pinene than in control mice. We detected less Ki67 and proliferation cell nuclear antigen in paraffin sections from xenograft tumor specimens taken from α-pinene-treated mice than in those from the control group. Meanwhile, α-pinene treatment induced apoptosis in xenograft tumors as determined by the TUNEL assay. Conclusions: These data strongly suggest that α-pinene inhibits prostate cancer growth in a xenograft model and may be an effective therapeutic agent for prostate cancer treatment.

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“…The results of this study are in accordance with previous study, which has reported that α-pinene as the major components in Cypress essential oil demonstrated strong cytotoxicity towards rat brain cancer cell line (N2a neuroblastoma cells). Significant decreases were observed in N2a cells at 100, 200 and 400 μg mL -1 (Aydin et al, 2013). Also, Matsuo et al (2011) revealed that α-pinene was able to induce apoptosis evidenced by early disruption of the mitochondrial potential, production of reactive oxygen species, and increase in caspase-3 activity in B16F10 murine melanoma cell.…”

Section: In Vivo Anticancer Activitymentioning

confidence: 91%

Chemical Composition, Antioxidant, Anticancer Properties and Toxicity Evaluation of Leaf Essential Oil of <i>Cupressus sempervirens</i>

Fayed

2015

Not Bot Hort Agrobot Cluj

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The essential oil isolated by hydro-distillation from Cupressus sempervirens (Cypress) leaves was analysed by GC-MS and tested for antioxidant and in vitro as well as in vivo anticancer activities. In addition, the toxicity effect of the essential oil was studied using normal Swiss mice. Eighteen components of Cypress essential oil were identified and the main essential oil components were α-pinene (29.21%), δ3-carene (18.92%), α-cedrol (12.25%), α-terpinolene (7.66%) and limonene (5.50%). Cupressus sempervirens essential oil was able to reduce the stable, purple-colored radical DPPH into yellow-colored DPPH reaching 50% of reduction with IC50 value = 290.09 μg mL -1. The in vitro anticancer activity of the essential oil was studied against two human promyelocytic leukemia cell lines (HL-60 and NB4) and experimental animals model cancer cell line (EACC). Cypress essential oil exerted the highest cytotoxic activity with a LC50 of 333.79 µg mL -1 against NB4 followed by HL-60 and EACC cell lines (LC50 of 365.41, and 372.43 µg mL -1 , respectively). Regarding in vivo anticancer study, pre-initiation treatment with the essential oil was more effective than initiation and post-initiation treatments respectively on the tumor (EACC) transplanted female mice (increase lifespan (%), decrease total EACC number and increase dead cells). In toxicity study, serum urea, transaminases and lactate dehydrogenase were increased. The results obtained from this study showed that the Cypress essential oil possesses antioxidant and anticancer properties, taking into consideration its mild toxicity.

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Antioxidative, anticancer and genotoxic properties of α-pinene on N2a neuroblastoma cells

Cited by 81 publications

References 55 publications

Essential oils from Egyptian aromatic plants as antioxidant and novel anticancer agents in human cancer cell lines

Essential oils from Egyptian aromatic plants as antioxidant and novel anticancer agents in human cancer cell lines

α-Pinene Inhibits Human Prostate Cancer Growth in a Mouse Xenograft Model

Chemical Composition, Antioxidant, Anticancer Properties and Toxicity Evaluation of Leaf Essential Oil of <i>Cupressus sempervirens</i>

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