Antioxidative Activity of Cornus officianalis Extracts Obtained by Four Different Extraction Techniques

Abstract

Oxidative stress leads to damage in all components of the cell, including proteins, lipids, and DNA due to imbalance between reactive oxygen species production and cellular detoxification. Phytochemicals are well-known to contain antioxidants, and their physiological role has been intensively studied. The fruit of Cornus officianalis has been used in oriental medicine and has been reported to have many functions. In this study, four different extraction techniques were applied to extract functional components from the fruit of Cornus officianalis, and the content of loganin, which is an antioxidant having DPPH radical and hydrogen peroxide scavenging activity and reducing power, was analyzed in each extract. Extraction techniques employed in this study were heat extraction by water, 70% ethanol extraction, enzyme treatment, and combination of enzyme treatment and heat extraction by water. All extracts contained 11.8-18.0 mg/g loganin and showed antioxidation function assayed by measuring DPPH radical and hydrogen peroxide scavenging activity and reducing power. Among them, heat extraction was the most effective technique, showing a maximum amount of loganin (18.0 mg/g) and antioxidative activity at 100 mg/ml concentration. Each extract showed very low cytotoxicity up to at 500 mg/ml but 10-20% cytotoxicity at 1,000 mg/ml by in vitro MTT assay.

Pharmacognostic outlooks on medical herbs of Sasang typology

“…35 Triterpenoid group of SY type-specific herbs are used for inflammations and arthritis for their anti-inflammatory, diuretic, and analgesic properties. In terpenoids, diaphoretic and anti-inflammatory, 39 antirheumatic, analgesic, and diuretic, 46 , 47 hypolipidemic and diuretic, 48 diuretic and stomachic, 39 , 49 expectorant, 50 , 51 diuretic and antimicrobial, 33 , 35 antipyretic, anti-inflammatory, and antimicrobial, 32 , 46 purgative, 52 diuretic, 50 diuretic, antitussive, and expectorant, 53 anti-inflammatory and antipyretic, 50 hypoglycemic, sedative, and immunostimulant, 50 , 54 and antioxidant and immunostimulant 55 activities were reported.…”

The Anti-Oxidative Effect of Suhwagije－decoction on the Liver and Spleen Cells of Aged Rats

“…3) NO in spleen 수화기제탕 약침액의 주요 구성 약물인 知母 26) , 黃柏 27) , 山茱萸 [28][29][30][31] , 覆盆子 32,33) , 柴胡 34) , 苦蔘 35,36) , 澤瀉 [37][38][39][40]…”