Antioxidative activity and synergistic effect of Thymus saturejoides Coss. essential oils with cefixime against selected food-borne bacteria

Kasrati, Ayoub; Jamali, Chaima Alaoui; Fadli, Meriam; Bekkouche, Khalid; Hassani, Lahcen; Wohlmuth, Hans; Leach, D. L.; Abbad, Abdelaziz

doi:10.1016/j.indcrop.2014.07.024

Cited by 69 publications

(34 citation statements)

References 41 publications

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“…Three standard antimicrobial agents, namely fluconazol, vancomycin and ciprofloxacin, in combination with each EO were used to evaluate their synergistic effect by the checkerboard assay method described by Nafis et al [29]. This synergy has been studied by determining the new MIC of antibiotics in the presence of EOs at a lower concentration (MIC/4), based on preliminary tests [47]. Briefly, an aliquot (50 µL) of each EO was added separately to microwells containing 50 µL of different antimicrobial concentrations, and inoculated with 100 µL of cell suspension.…”

Section: Evaluation Of Synergistic Effect Of the Eos With Conventionamentioning

confidence: 99%

A Comparative Study of the in Vitro Antimicrobial and Synergistic Effect of Essential Oils from Laurus nobilis L. and Prunus armeniaca L. from Morocco with Antimicrobial Drugs: New Approach for Health Promoting Products

et al. 2020

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Laurus nobilis L. (laurel, Lauraceae) and Prunus armeniaca L. (apricot, Rosaceae) are important industrial crops and display significant biological properties, including antimicrobial activity. In this work, essential oils (EOs) prepared from the leaves of both species from Morocco were evaluated for the first time for possible synergistic in vitro antibacterial and antifungal effects with some conventional antimicrobial drugs, namely fluconazole, ciprofloxacin and vancomycin. Samples were further evaluated for chemical composition by gas chromatography–mass spectrometry (GC-MS). The main volatile compounds detected in L. nobilis were eucalyptol (40.85%), α-terpinyl acetate (12.64%) and methyl eugenol (8.72%), while P. armeniaca was dominated essentially by (Z)-phytol (27.18%), pentacosane (15.11%), nonacosane (8.76%) and benzaldehyde (7.25%). Regarding antimicrobial activity, both EOs inhibited significantly all the microorganisms tested. The EO from L. nobilis had the highest activity, with minimal inhibitory concentrations (MICs) ranging from 1.39 to 22.2 mg/mL for bacteria and between 2.77 and 5.55 mg/mL for yeasts. Conversely, the combination of the studied EOs with ciprofloxacin, vancomycin and fluconazol resulted in a noteworthy decrease in their individual MICs. In fact, of the 32 interactions tested, 23 (71.87%) demonstrated total synergism and 9 (28.12%) a partial synergistic interaction. The EO from L. nobilis exhibited the highest synergistic effect with all the antibiotics used, with fractional inhibitory concentration (FIC) index values in the range of 0.266 to 0.75 for bacteria, and between 0.258 and 0.266 for yeast. The synergistic interaction between the studied EOs and standard antibiotics may constitute promising anti-infective agents useful for treating diseases induced by antibiotic-resistant pathogens.

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Section: Evaluation Of Synergistic Effect Of the Eos With Conventionamentioning

confidence: 99%

A Comparative Study of the in Vitro Antimicrobial and Synergistic Effect of Essential Oils from Laurus nobilis L. and Prunus armeniaca L. from Morocco with Antimicrobial Drugs: New Approach for Health Promoting Products

et al. 2020

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“…Although the oils had close sums of these two compounds, there were some discrepancies between their antioxidant activities. Generally, the antioxidant activity of EOs is the product of additive, synergistic and/or antagonistic effects, as they are complex mixtures of several classes of compounds [47][48][49]. Antioxidant activity varied depending on the method used for its evaluation and the time of extraction of the oil.…”

Section: Antioxidant Activitymentioning

confidence: 99%

Influence of Extraction Duration on the Chemical Composition and Biological Activities of Essential Oil of Thymus pallescens de Noé

et al. 2015

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Thymus pallescens de Noé essential oils were isolated from the plant's dried leaves, by hydrodistillation, with different distillation times (30 min, 1, 2 and 3 h). The essential oils compositions and their antioxidant, antimicrobial and insecticidal activities were examined. The chemical compositions of the oils were determined by GC and GC-MS. The antioxidant capacity was tested by four complementary methods: thiobarbituric acid reactive substances, ferric reducing power and scavenging of radicals DPPH • (2,2-diphenyl-1-picrylhydrazyl) and ABTS •+ [2,2 -azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)]. The antimicrobial activity was tested against three bacteria, two gram-positive (Staphylococcus aureus and Bacillus subtilis) and one gram-negative (Escherichia coli) and one yeast (Candida albicans). The insecticidal activity against confused flour beetle Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae) was evaluated using direct contact application and fumigant method. The predominant components in the oils were carvacrol (63.3-68.2 %), γ -terpinene (6.9-9.1 %), p-cymene (6.2-10.3 %) and thymol (6.5-7.5 %). The antioxidant activities were dependent on the time of hydrodistillation and the method used. The essential oils extracted for 30 min and 1 h were the most efficient against all the bacteria used. Toxicity tests against T. con-B Mohamed Hazzit

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“…is a dwarf shrub, up to 10 cm high, endemic in inner Anatolia, especially distributed in Sivas, growing in wild in open steppe on gypsous or poor fertile calcareous soils [12]. In terms of Pharmacological and phytochemical studies, many Thymus species have been reported for antioxidant, antimicrobial and other biological activities [13][14][15]. In addition, in the search for phytochemicals, plant parts are usually screened for phytochemicals that may be present.…”

Section: Introductionmentioning

confidence: 99%

Investigating of Phytochemicals, Antioxidant, Antimicrobial and Proliferative Properties of Different Extracts of Thymus spathulifolius Hausskn. and Velen. Endemic Medicinal Plant from Sivas, Turkey

Eruygur

Ataş

Çevir

et al. 2017

International Journal of Secondary Metabolite

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Thymus species has been used for antioxidant, antiseptic, antitussive, carminative, anti-inflammatory and antimicrobial activities as well as tonic and herbal teas. The present study was conducted to evaluate antioxidant, antimicrobial and proliferative properties of ethanol crude extract and fractions of Thymus spathulifolius (Hausskn. and Velen.) herbes. The antioxidant properties of ethanol extracts and fractions of Thymus spathulifolius were investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power, ferrous chelating activity, total flavonoid and total phenolic content analysis. Antimicrobial activity of the plant extracts were tested using the microdilution method, while proliferative activity were evaluated by MTT assay. Results showed that IC50 of T. spathulifolius extracts that scavenged 50% of the DPPH radical was found to be ranged from 62.39 to 1000 µg/mL. Hexane extract possessed moderate antimicrobial activity towards grampositive bacteria of S. aureus and fungi of C. albicans. S. aureus was the most sensitive bacteria than other tested microorganisms. The hexane and water extracts exhibited a good proliferative activity with ED50 of 3.28 µg/mL and 2.77 µg/mL, respectively. The antioxidant and antimicrobial activities, together with the ability of proliferation, provide some support for the T.spathulifolius's traditional use.

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Antioxidative activity and synergistic effect of Thymus saturejoides Coss. essential oils with cefixime against selected food-borne bacteria

Cited by 69 publications

References 41 publications

A Comparative Study of the in Vitro Antimicrobial and Synergistic Effect of Essential Oils from Laurus nobilis L. and Prunus armeniaca L. from Morocco with Antimicrobial Drugs: New Approach for Health Promoting Products

A Comparative Study of the in Vitro Antimicrobial and Synergistic Effect of Essential Oils from Laurus nobilis L. and Prunus armeniaca L. from Morocco with Antimicrobial Drugs: New Approach for Health Promoting Products

Influence of Extraction Duration on the Chemical Composition and Biological Activities of Essential Oil of Thymus pallescens de Noé

Investigating of Phytochemicals, Antioxidant, Antimicrobial and Proliferative Properties of Different Extracts of Thymus spathulifolius Hausskn. and Velen. Endemic Medicinal Plant from Sivas, Turkey

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