Antioxidative activities of hydrolysates from seabass skin prepared using protease from hepatopancreas of Pacific white shrimp

Senphan, Theeraphol; Benjakul, Soottawat

doi:10.1016/j.jff.2013.10.001

Cited by 82 publications

(64 citation statements)

References 31 publications

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“…Kittiphattanabawon et al (2012) also found the increased antioxidative activity of gelatin hydrolysate from blacktip shark skin prepared using papaya latex after being ingested in the simulated model system. The gelatin hydrolysate from seabass skin prepared using protease from hepatopancreas of Pacific white shrimp showed the increase of antioxidative activity with further hydrolysis in intestinal simulated system (Senphan and Benjakul 2014). On the other hand, FRAP of both NS-8GE and SS-8GE decreased after ingestion in the GIMs over digestion time of 0-4 h. The extensive hydrolysis during digestion might provide small peptides with low ability on donation of electron to free radicals.…”

Section: Resultsmentioning

confidence: 96%

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Antioxidative gelatin hydrolysate from unicorn leatherjacket skin as affected by prior autolysis

Karnjanapratum

Benjakul

2015

Int Aquat Res

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Gelatin hydrolysates from autolysed non-swollen and swollen unicorn leatherjacket skin prepared using partially purified glycyl endopeptidase (GE) from papaya latex were examined for their antioxidative activities. Autolysed swollen skin was more hydrolysed by GE as indicated by higher a-amino group content, compared with autolysed non-swollen skin. ABTS radical scavenging activity and ferric reducing antioxidant power (FRAP) of hydrolysates were increased with increasing levels of GE used. Antioxidative gelatin hydrolysate from autolysed skin, both non-swollen and swollen skins, using 8 % GE termed 'NS-8GE' and 'SS-8GE' exhibited different modes of action. When both hydrolysates were tested in lecithin liposome system, the efficiency in retardation of lipid oxidation was in a dose-dependent manner. Antioxidative activity of hydrolysates at 5.0 g/L was comparable to that of 0.1 g/L Trolox, in which the oxidation was almost completely inhibited. NS-8GE and SS-8GE also showed their antioxidative activities in gastrointestinal tract model system (GIMs). ABTS radical scavenging activity of both hydrolysates increased, but FRAP decreased in a duodenal condition. Based on size exclusion chromatography, major antioxidative peptides in NS-8GE and SS-8GE had molecular weight of 1,170 and 750 Da, respectively. Therefore, GE could enhance antioxidative activity of autolysed skin. Additionally, swelling process directly determined the modes of actions of resulting gelatin hydrolysates. NS-8GE had high ABTS radical scavenging activity, whereas SS-8GE showed high FRAP.

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Section: Resultsmentioning

confidence: 96%

“…NS-8GE and SS-8GE showed both radical scavenging activity and reducing antioxidant power. Gelatin hydrolysates from seabass and bigeye snapper skins displayed antioxidative activity in lecithin liposome systems (Senphan andBenjakul 2014, Phanturat et al 2010).…”

Section: Resultsmentioning

confidence: 99%

Antioxidative gelatin hydrolysate from unicorn leatherjacket skin as affected by prior autolysis

Karnjanapratum

Benjakul

2015

Int Aquat Res

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“…At higher concentrations, P1-1U had the highest DPPH radical scavenging activity, whilst P2-2U showed the lowest activity. DPPH radical scavenging activity was previously reported in protein hydrolysates prepared from the skin of seabass (Senphan and Benjakul 2014), catfish (Alemán et al 2011), Alaska pollack (Jia et al 2010) and bigeye snapper (Phanturat et al 2010). Increasing quantities of active peptides react with free radicals to convert them to stable products (Batista et al 2010).…”

Section: Dpph Radical Scavenging Activitymentioning

confidence: 90%

“…Gelatin hydrolysis was done either during gelatin extraction or after gelatin extraction according to the method of Senphan and Benjakul (2014) with a slight modification. Alcalase was used to hydrolyse gelatin and protease activity was determined as per the method of An et al (1994).…”

Section: Preparation Of Gelatin Hydrolysatementioning

confidence: 99%

“…During processing or dressing of seabass, skin is generated and considered as a byproduct. Recently, seabass skin has been used to produce collagen and gelatin, as well as hydrolysates with demonstrated bioactivities (Senphan and Benjakul 2014;Sinthusamran et al 2013). It is well known that protein hydrolysates and peptides can act as free radical scavengers, as well as transition metal chelators and exert antioxidant activities against enzymatic (lipoxygenase-mediated) and non-enzymatic peroxidation of lipids and fats (Sarmadi and Ismail 2010).…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant activities and selected characteristics of gelatin hydrolysates from seabass (Lates calcarifer) skin as affected by production processes

et al. 2015

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Antioxidant activities and selected characteristics of gelatin hydrolysates from seabass skin as affected by production processes were investigated. Hydrolysates were prepared using different processes, including hydrolysis during and after gelatin extraction. Samples hydrolysed during gelatin extraction showed a higher degree of hydrolysis (DH) and yield compared with those hydrolysed after gelatin extraction (p<0.05). All hydrolysates had a creamy yellowish colour. A lower abundance of volatile compounds was found in the hydrolysates produced during gelatin extraction, in comparison with those obtained after gelatin extraction. Hydrolysates prepared during gelatin extraction had higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidative power (FRAP) and ferrous ion chelating activity (p<0.05). Following a simulated in vitro gastrointestinal digestion, the DPPH radical scavenging activity and FRAP of the hydrolysates was retained, whilst ferrous ion chelating activity increased. The most appropriate conditions for the generation of antioxidant hydrolysates from seabass skin were identified.

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Byproducts from Fish Harvesting and Processing

Benjakul¹,

Sae‐leaw²,

Simpson³

2019

Byproducts From Agriculture and Fisheries

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Antioxidative activities of hydrolysates from seabass skin prepared using protease from hepatopancreas of Pacific white shrimp

Cited by 82 publications

References 31 publications

Antioxidative gelatin hydrolysate from unicorn leatherjacket skin as affected by prior autolysis

Antioxidative gelatin hydrolysate from unicorn leatherjacket skin as affected by prior autolysis

Antioxidant activities and selected characteristics of gelatin hydrolysates from seabass (Lates calcarifer) skin as affected by production processes

Byproducts from Fish Harvesting and Processing

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