Antioxidants, Oxyrase, and mitochondrial uncoupler 2,4-dinitrophenol improved postthaw survival of rhesus monkey sperm from ejaculates with low cryosurvival

Dong, Qiaoxiang; Tollner, Theodore L.; Rodenburg, Sarah E.; Hill, Dana L.; VandeVoort, Catherine A.

doi:10.1016/j.fertnstert.2010.04.017

Cited by 20 publications

(12 citation statements)

References 18 publications

(20 reference statements)

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“…Opening of mPTP is known to be induced during oxidative stress and ROS-mediated alterations to mPTP regulators and components were suggested to be responsible for this. Indeed, it was found that the mild uncoupling agent 2,4-dinitrophenol, which normally reduces ROS, improved motility in sperm with low cryopreservability [76] while the antioxidant, monothioglycerol was found to reduce mitochondria ROS as well as increase fertility and the percentage of cells with the ability to undergo acrosome reaction [41]. ROS-mediated mitochondrial permeabilization involved oxidative attack on the protein thiol groups on the mitochondrial membrane.…”

Section: Cryopreservation and Ros Productionmentioning

confidence: 99%

“…In one example, Dong et al reported that the beneficial effects of SOD administration were only seen in sperm with low post-thaw survivability [76]. This is also observed for other antioxidants namely coenzyme Q [218], resveratrol [219], zinc sulfate [220], ascorbic acid [150], catalase [76] and 2,4-dinitrophenol [76]. These studies concluded that the effectiveness of antioxidants was dependent on sperm quality.…”

Section: Effectiveness Of Antioxidants In Preventing Cryoinjury: Lessmentioning

confidence: 99%

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The roles of reactive oxygen species and antioxidants in cryopreservation

2019

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Cryopreservation has facilitated advancement of biological research by allowing the storage of cells over prolonged periods of time. While cryopreservation at extremely low temperatures would render cells metabolically inactive, cells suffer insults during the freezing and thawing process. Among such insults, the generation of supra-physiological levels of reactive oxygen species (ROS) could impair cellular functions and survival. Antioxidants are potential additives that were reported to partially or completely reverse freeze-thaw stress-associated impairments. This review aims to discuss the potential sources of cryopreservation-induced ROS and the effectiveness of antioxidant administration when used individually or in combination.

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Section: Cryopreservation and Ros Productionmentioning

confidence: 99%

Section: Effectiveness Of Antioxidants In Preventing Cryoinjury: Lessmentioning

confidence: 99%

The roles of reactive oxygen species and antioxidants in cryopreservation

2019

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“…It is known that high‐ROS levels can damage proteins, lipids, DNA, and membrane system . SOD, as well as catalase, peroxidase, glutathione peroxidase, glutathione reductase, and non‐enzymatic antioxidants together form a defense system to reduce the risk of ROS damage . SODs have recently found applications in the pharmaceutical and cosmetic industries, food, agricultural, and chemical industries .…”

Section: Introductionmentioning

confidence: 99%

Cloning, expression and biochemical characterization of recombinant superoxide dismutase from Antarctic psychrophilic bacterium Pseudoalteromonas sp. ANT506

Wang

Hou

et al. 2015

J. Basic Microbiol.

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In this study, a superoxide dismutase gene (PsSOD) from Pseudoalteromonas sp. ANT506 was cloned and over expressed in Escherichia coli. The PsSOD has an open reading frame of 582 bp with a putative product of 193 amino acid residue and an estimated molecular size of 21.4 kDa. His-tagged PsSOD was subsequently purified 12.6-fold by Ni-affinity chromatography and the yield of 22.9%. The characterization of the purified rPsSOD exhibited maximum activity at 30 °C and pH 8.0. The enzyme exhibited 13.9% activity at 0 °C and had high-thermo lability at higher than 50 °C. rPsSOD exhibited well capability to 2.5 M NaCl (62.4%). These results indicated that rPsSOD exhibited special catalytic properties.

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“…With the addition of glycerol, LDL at lower concentrations (2%-6%) yielded similar post-thaw motility (46%-54%) and AMI (28)(29)(30)(31)(32)(33)(34) as that of 8% LDL (53%66% for motility and 3368 for AMI) or 10% LDL (53%69% for motility and 36610 for AMI).…”

Section: Evaluation Of Ldl In Combination With Glycerolmentioning

confidence: 88%

The role of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in comparison with whole egg yolk for sperm cryopreservation in rhesus monkeys

Dong¹,

Rodenburg²,

Hill³

et al. 2011

Asian J Androl

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Low-density lipoprotein (LDL) extracted from hen egg yolk has recently been considered to be superior to whole egg yolk in sperm cryopreservation of various animal species. Meanwhile, there was a notion that high-density lipoprotein (HDL) in egg yolk may have a negative effect on post-thaw survival. The role of LDL and HDL in sperm cryopreservation of rhesus monkeys has not been explored. The present study evaluates their effect in comparison with egg yolk with or without the addition of permeable cryoprotectant (glycerol) on sperm cryopreservation of rhesus macaques. In addition, various additives intended to change the lipid composition of LDL-sperm membrane complex have also been tested for their effectiveness in preserving post-thaw viability. Our findings indicated that LDL is the main component in egg yolk that is responsible for its protective role for sperm cryopreservation in rhesus monkeys. Regardless of the presence or absence of glycerol, the protective role of LDL is similar to that of egg yolk and we did not observe any superiority in post-thaw survival with LDL when compared to egg yolk. Modifying the lipid composition of LDL-sperm membrane complex with the addition of cholesterol, cholesterol loaded cyclodextrin and phosphatidylcholine also did not yield any improvements in post-thaw survival; while addition of methyl-b-cyclodextrin reduced post-thaw motility. HDL plays a neutral role in sperm cryopreservation of rhesus monkeys. The present study suggests that egg yolk may still hold advantages when compared with LDL as effective components in extenders for sperm cryopreservation in rhesus monkeys.

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Antioxidants, Oxyrase, and mitochondrial uncoupler 2,4-dinitrophenol improved postthaw survival of rhesus monkey sperm from ejaculates with low cryosurvival

Cited by 20 publications

References 18 publications

The roles of reactive oxygen species and antioxidants in cryopreservation

The roles of reactive oxygen species and antioxidants in cryopreservation

Cloning, expression and biochemical characterization of recombinant superoxide dismutase from Antarctic psychrophilic bacterium Pseudoalteromonas sp. ANT506

The role of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in comparison with whole egg yolk for sperm cryopreservation in rhesus monkeys

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