Antioxidants in Food: The Significance of Characterisation, Identification, Chemical and Biological Assays in Determining the Role of Antioxidants in Food

Parsons, Barry J.

doi:10.3390/foods6080068

Cited by 13 publications

(7 citation statements)

References 8 publications

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“…Parry et al (2005) evaluated the amount of total phenolic content for cold pressed berry seed oils and the values were found to be 1.73-2.0 mg GAE/g sample (34). To further confirm the antioxidant properties of cold pressed seed oils, ABTS + radical scavenging activity, which is one of the most commonly used methods, was evaluated (35). Different antioxidant activity methods were developed due to the complexity of the oxidation processes, such as DPPH, ABTS radical scavenging activity, oxygen radical absorbance capacity (ORAC), cupric ion reducing antioxidant capacity (CUPRAC) (36,37) etc.…”

Section: Resultsmentioning

confidence: 99%

The Comparison of the Quality Properties of Some Commercial Cold Pressed Seed Oils

Özyurt

2019

Journal of the Turkish Chemical Society Section A: Chemistry

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In this study, the aim is to explain some quality properties of five different kinds of seed oils produced by the cold pressed extraction method. Coffee bean, pomegranate seed, cherry seed, apricot seed, and fig kernel cold pressed oils that are produced in the domestic market of Turkey were evaluated in terms of their some quality characteristics such as peroxide values, free fatty acidity, and UV-specific extinction values, total phenolic compounds and antioxidant activity. The highest levels of peroxide values and free fatty acid values were determined in coffee bean oil with abundances of 26.34 mEqO2/kg oil and 2.07 mg KOH/g oil, respectively. Coffee bean oil also had relatively high UV-specific extinction values (K232 values 4.04 and K270 values 3.99). In the results of the total phenolic compound analysis performed to recognize the effects on health, this value was highest in coffee bean oil, with 1380 mg GAE/L oil. The results of the highest antioxidant activity were examined in the fig kernel oil. These values were compared with the studies in the literature and evaluated according to the legal boundaries.

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Section: Resultsmentioning

confidence: 99%

The Comparison of the Quality Properties of Some Commercial Cold Pressed Seed Oils

Özyurt

2019

Journal of the Turkish Chemical Society Section A: Chemistry

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“…Bulgarian and Italian gerger studied for the total phenolic content, and the antioxidant potential of ethanol extract suggested a higher antioxidant capacity in Bulgarian gerger than the Italian variety [24]. The method of extraction of food antioxidants affects the nature of compounds isolated, and the quality of the total antioxidant activity [42]. Compounds reported in the soxhlet aided methanol extract of gerger leaves were absent in the gerger leaf decoction reported in this study.…”

Section: Journal Of Food and Nutrition Researchmentioning

confidence: 49%

Establishing Gerger (<i>Eruca sativa</i>) Leaves as Functional Food by GC-MS and <i>In-vitro</i> Anti-lipid Peroxidation Assays

Bratty¹,

Thangavel²,

Shar³

et al. 2020

JFNR

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Conventional food that safeguards against chronic illnesses is known as a functional food. Establishing functional foods starts with phytoconstituent analysis and in-vitro characterization of health benefits. Eruca sativa, popularly known as gerger or jarjeer in Saudi Arabia, is an annual edible shrub cultivated worldwide. Gerger leaves are consumed raw in salads and have additional health benefits. This study investigated the phytochemical profile of aqueous decoction of gerger leaves of the Saudi origin by GC-MS assay. We also performed in-vitro anti-lipid peroxidation and total antioxidant capacity assays using gerger decoction. Twenty-seven chemical compounds belonging to seven classes constituted the gerger decoction: organic siloxanes (39.75%), organic silyl esters (18.28%), phenolics (17.87%), aromatic and aliphatic esters (10.48%), terpenoids (7.09%), heterocycles (3.83%), and sulfur compounds (2.70%). This study reported the presence of compounds mentioned above for the first time in gerger leaves. The decoction method was efficient in the extraction of heat-stable terpenoids like astaxanthin (2.23%), cilonasterol (1.48%), ingol-12-acetate (0.4%), and phytol (2.98%). The in-vitro anti-lipid peroxidation study demonstrated the ability of gerger decoction to inhibit hepatic lipid peroxidation in a significantly dose-dependent (150 to 400 μg/ml) manner compared to quercetin. A dose of 400 μg/ml of gerger decoction resulted in 68.46 ± 0.01% inhibition of oxidation of hepatic lipids. The total antioxidant capacity of gerger leaves reported as the IC 50 of the decoction was 217.90 ± 2.2 μg/ml and also statistically significant. The in-vitro models suggested the antioxidant mechanism of gerger was by hydrogen atom transfer and reduction of metal ions. The study substantiated that gerger is a functional food besides established the phytochemical profile contributing to the antioxidant activity. Given that the gerger decoction has a high silicon content and antioxidants, attempt to determine the bioavailability and to identify the molecular targets are essential to overcome bone disorders and oxidative stress.

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“…Based in these findings, Myrtaceae fruit peels may show potential antioxidant capacity and may be considered a valuable source of natural antioxidants, preventing the harmful effect of free radicals. Free radicals and reactive oxygen species (ROS) have been considered to contribute to the progress of aging and illness by causing oxidative stress [ 45 , 46 ]. Aimed at improving health, the consumption of these natural sources of substances can be vitally important to the human body.…”

Section: Resultsmentioning

confidence: 99%

“…Few studies report the influence of Myrtaceae fruits on the antiproliferative effects on cancer cells. It has been reported that several flavonoid compounds from M. cauliflora presented antiproliferative activities against HT-29 (IC 50 = 65 μM) and HCT116 (IC 50 = 30 μM) colon cell lines [ 45 ]. Also, there are some findings concerning S. cumini extracts that reported the inhibition on the colon cancer line (HCT116).…”

Section: Resultsmentioning

confidence: 99%

Antiproliferative Activity on Human Colon Adenocarcinoma Cells and In Vitro Antioxidant Effect of Anthocyanin-Rich Extracts from Peels of Species of the Myrtaceae Family

et al. 2021

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There is a significant indication of the beneficial health effects of fruit rich diets. Fruits of native plant species have noticeably different phytochemicals and bioactive effects. The aim of this work was to characterize and compare the constituents of jabuticaba (Myrciaria jaboticaba, MJ), jamun-berry (Syzygium cumini, SC), and malay-apple (Syzygium malaccense, SM) extracts and their influence on antioxidant activity in vitro and antiproliferative effects on human colon adenocarcinoma cells. According to the results, dried peel powders (DP) have a high anthocyanin content, phenolic compounds, and antioxidant activity when compared to freeze dried extracts (FD). M. jaboticaba dried peel powder extract had a higher total anthocyanin and phenolic compounds content (802.90 ± 1.93 and 2152.92 ± 43.95 mg/100 g, respectively). A reduction in cell viability of HT-29 cells after treatment with M. jaboticaba extracts (DP-MJ and FD-MJ) was observed via MTT assay. Flow cytometry showed that the treatment with the anthocyanin-rich extracts from MJ, SC, and SM had an inhibitory impact on cell development due to G2/M arrest and caused a rise in apoptotic cells in relation to the control group. The findings of this study highlight the potential of peel powders from Myrtaceae fruits as an important source of natural antioxidants and a protective effect against colon adenocarcinoma.

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Antioxidants in Food: The Significance of Characterisation, Identification, Chemical and Biological Assays in Determining the Role of Antioxidants in Food

Abstract: There is a vast research literature on the antioxidant activity in food. [...]

Cited by 13 publications

References 8 publications

The Comparison of the Quality Properties of Some Commercial Cold Pressed Seed Oils

The Comparison of the Quality Properties of Some Commercial Cold Pressed Seed Oils

Establishing Gerger (<i>Eruca sativa</i>) Leaves as Functional Food by GC-MS and <i>In-vitro</i> Anti-lipid Peroxidation Assays

Antiproliferative Activity on Human Colon Adenocarcinoma Cells and In Vitro Antioxidant Effect of Anthocyanin-Rich Extracts from Peels of Species of the Myrtaceae Family

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