Antioxidants in Colostrum and Milk of Sows and Cows

Abstract

On account of the oxidative stress conditions that may appear during parturition, colostrum should provide with not only nutritional and immunological components but also antioxidative protection of newborn. There is evidence that apart from well-known antioxidative enzymes like glutathione peroxidase, superoxide dismutase, catalase or low molecular antioxidants, proteins like lactoperoxidase (LPO), lactoferrin (LF) and ceruloplasmin (CP) may exert antioxidative properties in colostrum. The aim of present study was to determine and to evaluate LPO, LF and CP activities in colostrum and milk of sows and cows. Samples were collected from 16 healthy cows five times: immediately after parturition, 12, 24 and 48 h, and 7 days postpartum as well as from 14 healthy sows five times: immediately after parturition, 6, 12, 24 and 36 h later. Examined parameters were determined spectrophotometrically at 412, 560 and 540 nm respectively. LPO activity was higher in sows as in cows and increased significantly within examined time. LF ability to inhibit superoxide radical generation was higher in sows as in cows and increased significantly within examined time. CP oxidase activity was higher in cows as in sows and decreased significantly during experimental period. In conclusion, antioxidative defence system in colostrum shows dynamic changes that allow for providing with necessary protection from oxidative stress conditions, which may appear after parturition.

Activities of N-acetyl-β-d-glucosaminidase and glutathione peroxidase in bovine colostrum and milk

“…Antioxidative activity of ceruloplasmin decreased, antioxidative activity of lactoferrin increased while the activity of lactoperoxidase fluctuated with a tendency to increase during the examined period (Albera and Kankofer, 2009). It can confirm the present results suggesting that the antioxidative system reacts in accordance with the current situation.…”

“…Milk samples were collected 6 and 12 days post partum. The time of sample collection was selected on the basis of literature where no data on several time points for sample collection was found and of our own previous studies and the possibility to register dynamic changes in the activity of examined parameters after parturition (Kankofer and Lipko, 2008;Albera and Kankofer, 2009).…”

“…Changes in the contents of its major nutritive components during lactation are well known in humans (Allen et al, 1991) as well as in domestic animals such as cows (Rook and Campling, 1965;Hanuš et al, 2010). But colostrum contains also many biofunctional constituents, including growth factors, antipathogenic compounds and immune enhancing components, as well as antioxidants and their action during lactation still requires elucidation (Lindmark-Mansson and Akesson, 2000;Albera and Kankofer, 2009). The biological role of colostrum has therefore been suggested to provide not only nutrients but also anti-infection factors against pathogenic bacteria and viruses for the newborn whose immune system is not fully developed yet as well as antioxidative defence against reactive oxygen species (ROS).…”

Antioxidant defence of colostrum and milk in consecutive lactations in sows

“…Data obtained from the cow colostrum and the milk showed dynamic changes in total antioxidant capacity (TAC) within 7 days postpartum [9]. Albera and Kankofer [10] compared the activity of lactoperoxidase, lactoferrin, and ceruloplasmin between cows and sows.…”

Fatty Acid Composition, Fat Globule Size and Reactive Oxygen Species-Scavenging Activity of Mare Milk: A Longitudinal Study

“…Some studies are based on the evaluation of antioxidant potential of HM [16] and CM [17], but little is known about the MM potential and the effect of period of lactation on it.…”