Activities of N-acetyl-β-d-glucosaminidase and glutathione peroxidase in bovine colostrum and milk

Abstract

Parturition and post partum period are susceptible for antioxidative/oxidative imbalance as well as inflammatory processes related to either uterus or mammary gland. Fifteen Holstein-Friesian cows were used to examine the relationship between the antioxidant enzyme glutathione peroxidase (GSH-Px) and inflammatory enzyme N-acetyl-glucosaminidase (NAGase) in colostrum and milk during 12 days after parturition. Samples from each udder quarter were collected immediately after parturition, after 24 h and 48 h as well as after 6 and 12 days post partum. The activity of GSH-Px was measured spectrophotometrically and NAGase spectrofluorimetrically. NAGase activity generally remained constant during the examined period confirming no inflammation and healthy udder. Moreover, no significant differences in the activity among quarters were detected. The activity of GSH-Px rose significantly (P < 0.05) in the examined period suggesting the dynamic balance of antioxidative defence. No differences among quarters confirmed that in healthy quarters metabolic processes are on a similar level but positive correlations between the antioxidative and inflammatory enzyme in quarters and with regard to time may suggest a possible relationship during inflammation.

Comparative analysis of buffalo and cow milk for quality characteristics and β-N-acetyl-glucosaminidase activity in non-infected animals

“…Established on the results of the present-day study, a standard value of 0•1-1•04 pmoles 4-MU/min/µL for normal milk NAGase activity in cows with ≥30 DIM (196 samples) could be suggested. The previous conclusion that there was no significant variation in NAGase activity of all four quarters after parturition (Kankofer et al, 2010) was supported by the interpretations of the present study.…”

“…The milk comprises of several enzymes. Milk along with serum and other body fluids contain a lysosomal enzyme, β-N-acetyl-glucosaminidase (NAGase; EC 3.2.1.30) (Kankofer et al, 2010;Leitner et al, 2001). In case of clinical mastitis, NAGase activity is usually increased or just reach up to its topmost value (Fitz-Gerald et al, 1981;Leitner et al, 2003).…”

Evaluation of the relationship between milk glutathione peroxidase activity, milk composition and various parameters of subclinical mastitis under seasonal variations

“…GSH-Px is one of the principal enzymatic defence systems in controlling the free radicals induced by neutrophils (HAMED et al, 2008). However, GSH-Px deficiency leads to an increase in oxidative stress intensity (KANKOFER et al, 2010).…”

“…The data obtained from the cited literature provide conflicting results (HAMED et al, 2008;MATEI et al, 2011;YANG et al, 2011). Some studies have investigated the activity of GSH-Px in bovine colostrum, milk and blood over a short period after calving, and in specific conditions such as clinical or subclinical mastitis (KANKOFER et al, 2010;ANDREI et al, 2011;ERISIR et al, 2011;KONVICNA et al, 2015). GSH-Px activity at different stages of lactation has also been determined (PILARCZYK et al, 2012).…”

“…Glutathione peroxidase (GSH-Px) is a selenocysteine-containing protein that plays an important role in the cellular defence against oxidative stress (KANKOFER et al, 2010). However, GSH-Px, as an indicator of oxidative stress, catalyzes the reduction of various hydrogen and lipid peroxides (CELI, 2011).…”