Antioxidants and iron chelators inhibit oxygen radical generation in fungal cultures of plant pathogenic fungi

Sebestyen, Dana; Pérez-González, Gabriel; Goodell, Barry

doi:10.1016/j.funbio.2022.04.002

Cited by 4 publications

(13 citation statements)

References 55 publications

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“…trabeum (Figure ), suggesting that the decrease in the mass loss of the wood samples treated with either DTPA or IDS was due to the restriction of the degradation pathways by the chelators. This finding is inconsistent with a previous report, demonstrating that chelators significantly inhibit mycelial growth of Phaeoacremonium minimum at a loading dose of 1461 mg/L . In comparison, the 200 mg/L concentration of chelators used in our study was much lower than that used previously, suggesting that chelators inhibit fungal growth only at a high concentration, which was not used in this work.…”

Section: Results and Discussioncontrasting

confidence: 99%

“…Previous studies have shown that the antioxidant compounds, butylated hydroxytoluene (BHT) and t-butylhydroquinone, are capable of enhancing the efficiency of organic biocides in wood preservation. 13,14 This enhanced effect, as speculated previously, 15,16 is due to the ability in scavenging free radicals generated by the nonenzymatic system during brown-rot fungal decay. However, organic biocides are only enhanced after high loadings of antioxidants, 13,14 suggesting that a more efficient approach to enhance organic biocides is needed.…”

Section: ■ Introductionsupporting

confidence: 54%

“…Notably, the concentration of DTPA and IDS (∼0.02%) used for wood impregnation was much lower than that (BHT, EDTA, or other antioxidants, ∼2 wt % or higher) used previously. 13,14,16 The primary reason for this difference is that scavenging free radicals is less cost-effective in alleviating fungal oxidative stress compared to deactivating iron. This data suggests that it is feasible to enhance the performance of organic biocides in protecting wood against brown-rot fungi using the chelators with high iron affinity.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…A recent study proposed that chelators with high iron affinity cannot always restrict nonenzymatic attack because some chelators can reduce iron. 16 For example, 2,3 dihydroxybenzoic acid (2,3-DHBA) is an iron chelator with a strong ability to promote iron reduction. 29 This finding suggests that chelators should be screened to construct a compound system consisting of organic biocides and chelators to preserve wood.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…23,24 The generation of free radicals is sustained, making it less cost-effective to restrict CMF chemistry by scavenging free radicals, as widely reported previously. 15,16 As iron sequestration and reduction are essential to sustainably produce free radicals, 21,25 limiting iron availability and reactivity is a more efficient way to restrict nonenzymatic CMF chemistry. Therefore, we hypothesized that chelators with high iron affinity would improve the stability and efficiency of organic biocides in protecting wood against brown-rot fungi.…”

Section: ■ Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Novel and Green System for Protecting Wood against Gloeophyllum trabeum by Combining Biodegradable Isothiazolinone with Nontoxic Chelators

Cheng

et al. 2022

ACS Sustainable Chem. Eng.

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Impregnation with biocides is an efficient approach to prolong the service life of wood materials, but most biocides pose a toxic threat to human health and the environment. This study presents a novel and green system for protecting Masson pine and pond cypress against the brown-rot fungus Gloeophyllum trabeum through a combination of 4,5-dichloro-2-n-octyl-3isothiazolinone (DCOI) and chelators with high iron affinity. As results, both diethyltriaminepentaacetic acid (DTPA) and tetrasodium iminodisuccinate (IDS) reduced the mass losses of Masson pine and pond cypress, but neither chelator inhibited the growth of G. trabeum at a low concentration. The compound system was more efficient in reducing the mass loss of Masson pine compared to DCOI alone. Moreover, the DCOI degradation rate decreased significantly by IDS, suggesting that chelators with high iron affinity can protect organic biocides from degradation. All data supported that the chelators were able to enhance the organic biocide in improving wood decay resistance, and the involved mechanism was mainly due to the restriction on iron-based Fenton chemistry deployed by brown-rot fungi. This study provides valuable insight into the development of environmentally benign techniques to preserve wood.

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Section: Results and Discussioncontrasting

confidence: 99%

Section: ■ Introductionsupporting

confidence: 54%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Novel and Green System for Protecting Wood against Gloeophyllum trabeum by Combining Biodegradable Isothiazolinone with Nontoxic Chelators

Cheng

et al. 2022

ACS Sustainable Chem. Eng.

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Comparison of the Efficacy of Trichoderma and Bacillus Strains and Commercial Biocontrol Products against Grapevine Botryosphaeria Dieback Pathogens

et al. 2023

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Grapevine trunk diseases (GTDs) cause significant yield losses worldwide and limit the lifespan of vineyards. In the last few years, using biological control agents (BCAs) for pruning wound protection has become a promising management strategy for the control of these pathologies. This study aimed to compare the antifungal activities of a grapevine-native Trichoderma harzianum isolate and a high-potential Bacillus velezensis strain against two pathogenic Botryosphaeriaceae species in artificially inoculated, potted, grafted plants under controlled greenhouse conditions, taking three commercial biocontrol products (based on T. atroviride I-1237, T. harzianum T-22, and Bacillus subtilis BS03 strains) as a reference. To reproduce certain field conditions more realistically, inoculation of the protective agents and the pathogens was conducted simultaneously immediately after pruning instead of allowing the BCAs to colonize the wounds before pathogen inoculation. Significant differences in necrosis lengths were detected for both Neofusicoccum parvum- and Diplodia seriata-infected plants, and a remarkable protective effect of Bacillus velezensis BUZ-14 was observed in all cases. Trichoderma-based treatments showed different efficacies against the two pathogenic fungi. While the three tested BCAs resulted in significant reductions in vascular necrosis caused by N. parvum, they did not significantly reduce D. seriata infection compared to the untreated inoculated control. The B. subtilis strain was not effective. The reported results provide support for the potential Bacillus velezensis may have for pruning wound protection against Botryosphaeriaceae fungi, encouraging its evaluation under natural field conditions.

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First Description of Non-Enzymatic Radical-Generating Mechanisms Adopted by Fomitiporia mediterranea: An Unexplored Pathway of the White Rot Agent of the Esca Complex of Diseases

Moretti

Goddard

Puca

et al. 2023

JoF

Self Cite

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Fomitiporia mediterranea (Fmed) is the primary Basidiomycota species causing white rot in European vineyards affected by the Esca complex of diseases (ECD). In the last few years, an increasing number of studies have highlighted the importance of reconsidering the role of Fmed in ECD etiology, justifying an increase in research interest related to Fmed’s biomolecular pathogenetic mechanisms. In the context of the current re-evaluation of the binary distinction (brown vs. white rot) between biomolecular decay pathways induced by Basidiomycota species, our research aims to investigate the potential for non-enzymatic mechanisms adopted by Fmed, which is typically described as a white rot fungus. Our results demonstrate how, in liquid culture reproducing nutrient restriction conditions often found in wood, Fmed can produce low molecular weight compounds, the hallmark of the non-enzymatic “chelator-mediated Fenton” (CMF) reaction, originally described for brown rot fungi. CMF reactions can redox cycle with ferric iron, generating hydrogen peroxide and ferrous iron, necessary reactants leading to hydroxyl radical (•OH) production. These observations led to the conclusion that a non-enzymatic radical-generating CMF-like mechanism may be utilized by Fmed, potentially together with an enzymatic pool, to contribute to degrading wood constituents; moreover, indicating significant variability between strains.

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Antioxidants and iron chelators inhibit oxygen radical generation in fungal cultures of plant pathogenic fungi

Cited by 4 publications

References 55 publications

Novel and Green System for Protecting Wood against Gloeophyllum trabeum by Combining Biodegradable Isothiazolinone with Nontoxic Chelators

Novel and Green System for Protecting Wood against Gloeophyllum trabeum by Combining Biodegradable Isothiazolinone with Nontoxic Chelators

Comparison of the Efficacy of Trichoderma and Bacillus Strains and Commercial Biocontrol Products against Grapevine Botryosphaeria Dieback Pathogens

First Description of Non-Enzymatic Radical-Generating Mechanisms Adopted by Fomitiporia mediterranea: An Unexplored Pathway of the White Rot Agent of the Esca Complex of Diseases

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