Antioxidant vitamins C, E and coenzyme Q10 vs Dexamethasone: comparisons of their effects in pulmonary contusion model

Abstract

BackgroundThe goal of our study is to evaluate the effects of antioxidant vitamins (vitamin C and E), Coenzyme Q10 (CoQ10) and dexamethasone (Dxm) in experimental rat models with pulmonary contusion (PC).MethodsRats were randomly divided into six groups. Except for the control, all subgroups had a moderate pulmonary contusion. Animals in the group I and group II received intraperitoneal saline, group III received 10mg.kg-1 CoQ10 group IV received 100mg.kg-1 vitamin C, group V received 150mg.kg-1 vitamin E, and group VI received 10mg.kg-1 Dxm. Blood gas analysis, serum nitric oxide (NO) and malondialdehyde (MDA) levels as well as superoxide dismutase (SOD) activity assays, bronchoalveolar lavage (BAL) fluid and histopathological examination were performed.ResultsAdministration of CoQ10 resulted in a significant increase in PaO2 values compared with the group I (p = 0.004). Levels of plasma MDA in group II were significantly higher than those in the group I (p = 0.01). Early administration of vitamin C, CoQ10, and Dxm significantly decreased the levels of MDA (p = 0.01). Lung contusion due to blunt trauma significantly decreased SOD activities in rat lung tissue compared with group I (p = 0.01). SOD levels were significantly elevated in animals treated with CoQ10, Vitamin E, or Dxm compared with group II (p = 0.01).ConclusionsIn our study, CoQ10, vitamin C, vitamin E and Dxm had a protective effect on the biochemical and histopathological outcome of PC after experimental blunt thorax trauma.

Desferrioxamine Reduces Oxidative Stress in the Lung Contusion

“…Various and numerous free oxygen radicals and proteolytic and lipolytic enzymes cause cellular breakdown [7, 8, 26]. The lung contusion models in rats demonstrated that MDA levels increase in lung tissue as a final product of cellular membrane lipids' peroxidation [23, 27]. In our study, we found that the MDA levels were significantly higher in the contusion group than other groups.…”

“…In our study, we found that the MDA levels were significantly higher in the contusion group than other groups. Similarly, previous studies conducted with experimental lung contusion models have shown the existence of oxidative stress in the pathogenesis of contusions [23, 27]. …”

“…In our investigation of the literature, we found no publications relating to iNOS' role in lung contusions. On the other hand, Gokce et al [27] found an increase in NO levels in the blood of rats following contusions. In addition, it was reported that peroxynitrite increases in the lungs following contusions, which triggers inflammation and increases tissue hypoxia by causing vasoconstriction [44].…”

The potential protective role of coenzyme q10 on the cyclophosphamide-induced lung toxicity in adult male albino rats: a histological and ultrastructural study

“…33 Gokce et al reported that coenzyme Q10 is the only antioxidant that is fat soluble, synthesized endogenously and is present in tissues in the active form. 34 Moreover, Turunen et al stated that coenzyme Q10 is a mediator of lipid peroxidation and an essential cofactor in the electron transport chain and a component of the lipid membranes. 16 These effects might explain the notable reduction of the apoptotic cells expressed by marked decrease of caspase 3 expression within the alveolar and inter-alveolar tissues in this study in the lungs of rats of group IV (treated with Gokce et al stated that the strong antioxidant coenzyme Q10 plays a role in membrane stabilization as it is a component of the mitochondrial electron transport chain.…”

“…Also, it inhibits lipid peroxidation by preventing a peroxidation chain reaction and/or picking up ROS. 34 Lim et al conceived that coenzyme Q10 might attenuate the release of tumor necrosis factor alpha (TNFα) through its ability to limit the membrane lipid peroxidation with its subsequent pro-inflammatory signaling. Also, being a vital component of the respiratory chain, it plays a key role in the synthesis of cellular ATP.…”