Antioxidant status in patients with psoriasis

Abstract

Psoriasis is a chronic inflammatory skin disease with an unknown aetiology that has been associated with abnormal plasma lipid metabolism and oxidative stress. There are controversial results in the previous studies investigating oxidant/antioxidant systems in psoriasis.The aim of this work was to evaluate the plasma levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), total bilirubin (T. Bili), direct bilirubin (D. Bili), uric acid (UA), apolipoproteins (ApoA1 and ApoB), Lp(a) and activities of paraxonase 1 (PON1) in 100 patients with psoriasis and 100 controls, and to look for a correlation between these parameters in psoriasis.PON1, bilirubin and UA were measured spectrophotometrically, MDA by the high-performance liquid chromatography method, apolipoproteins and Lp(a) by immunoprecipitation assays, and lipid and other biochemical parameters were determined by routine laboratory methods.In patients with psoriasis, there was a significant decrease in PON1, SOD and CAT activities (P < 0.05) and an increase in MDA levels (P < 0.01). Also, the levels of bilirubin (total and direct) and UA were decreased in patients with psoriasis but were not significant (P > 0.05).These results suggest that psoriasis was in a state of oxidative stress and that the protective effects of high-density lipoprotein against atherosclerosis may be dependent on PON1 activity. Moreover, there is a negative correlation between antioxidant with Lp(a), apoB and MDA levels, suggesting that subjects with higher levels of Lp(a) and apoB and lower levels of antioxidant are more exposed to oxidative damage. These findings may explain in part the reported increase in cardiovascular mortality in psoriasis.

Clinical Value of Antioxidant Enzymes and Oxidative Biomarkers in Psoriasis

“…Eski çalışmalarda çelişkili sonuçlar olmasına rağmen, oksidatif stresin neden olduğu hastalıklardan birinin de psöriasis olduğunu bildiren çalışmalar mevcuttur (4,(6)(7)(8)(9) . Oksidatif stres psöriasis patogenezi için temel teorilerden biridir.…”

“…Çalışmamızda, oksidatif biyobelirteçlerin kontrol grubuna göre yüksek olması psöriasisteki oksidatif stresi desteklemektedir. Patogenezde oksidatif stres ile ilgili çelişkili sonuçların yeni tanı almış hasta, çalışma metodu, alınan örnek veya ilaç kullanan hastalar nedeni ile olabileceği belirtilmiştir (9) . Bu yüzden çalışmamızdaki hasta grubu son üç ayda herhangi bir tedavi almamış psöriasisli hastalardan seçildi.…”

“…Yapılan çalışmalarda, hastalık şiddeti ile oksidatif stres belirteçleri arasında pozitif, antioksidan belirteç-ler arasında negatif korelasyon bulunmuştur (4,9) . Ça-lışmamızda, hem antioksidatif enzim aktiviteleri hem de oksidasyon biyobelirteç düzeyleri orta-şiddetli psöriasis hastalarında daha yüksek saptandı.…”

“…Oksidatif stres psöriasis için patofizyolojik bir neden olmasına karşılık, bu alanda birçok biyobelirteç kullanılmış ve çelişkili sonuçlar bulunmuştur (4,(6)(7)(8)(9)(10)(11) . Bazı çalışmalarda artan belirteçler, bazı çalışmalarda ya azalmış ya da değişmemiştir.…”

Evaluation of changes in expression pattern of oxidative stress genes under the influence of adalimumab

“…SOD catalyzes the dissociation of superoxide anion radicals to hydrogen peroxide and oxygen, whereas CAT is responsible for the distribution of hydrogen peroxide toxic to the body to water and oxygen (Visir & Makurina, ). Their reduced activity in patients with psoriasis compared to healthy persons also leads to the conclusion of the participation of oxidative stress in the etiology of psoriasis (Houshang et al, ). An example of a commonly used anticytokine drug in the treatment of moderate to severe psoriasis is adalimumab—a TNF‐α inhibitor.…”