Antioxidant responses to azinphos methyl and carbaryl during the embryonic development of the toad Rhinella (Bufo) arenarum Hensel

Ferrari, Alejandro; Lascano, Cecilia Inés; Anguiano, Olga L.; D’Angelo, Alessandra; Venturino, Andrés

doi:10.1016/j.aquatox.2009.03.003

Cited by 45 publications

(37 citation statements)

References 60 publications

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“…Afterward, no additional signs of acute toxicity or mortality were observed. On the contrary, other toxicants such as pesticides are able to cause malformations along with oxidative stress in R. arenarum embryos [32]. This is also supported by the fact that the exposure from day 4 of development on (heartbeat stage) showed a significantly higher resistance to As compared with exposure from fertilization.…”

Section: Discussionmentioning

confidence: 79%

“…The significant decrease in total reactive antioxidant potential in R. arenarum embryos suggests a decrease in the ability of the antioxidant system to overcome the oxidative stress elicited by the toxicant. Ferrari et al [32] described the induction of GST activity attributable to pesticide exposure at the end of R. arenarum embryonic development (complete operculum stage). Conversely, an antioxidant response is evidenced by the induction of GST activity and the increase in GSH content.…”

Section: Discussionmentioning

confidence: 99%

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Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum

Mardirosian

Lascano

Ferrari³

et al. 2015

Enviro Toxic and Chemistry

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Arsenic (As), a natural element of ecological relevance, is found in natural water sources throughout Argentina in concentrations between 0.01 mg/L and 15 mg/L. The autochthonous toad Rhinella arenarum was selected to study the acute toxicity of As and the biochemical responses elicited by the exposure to As in water during its embryonic development. The median lethal concentration (LC50) value averaged 24.3 mg/L As and remained constant along the embryonic development. However, As toxicity drastically decreased when embryos were exposed from heartbeat-stage on day 4 of development, suggesting the onset of detoxification mechanisms. Given the environmental concentrations of As in Argentina, there is a probability of exceeding lethal levels at 1% of sites. Arsenic at sublethal concentrations caused a significant decrease in the total antioxidant potential but generated an increase in endogenous glutathione (GSH) content and glutathione S-transferase (GST) activity. This protective response might prevent a deeper decline in the antioxidant system and further oxidative damage. Alternatively, it might be linked to As conjugation with GSH for its excretion. The authors conclude that toad embryos are more sensitive to As during early developmental stages and that relatively high concentrations of this toxic element are required to elicit mortality, but oxidative stress may be an adverse effect at sublethal concentrations.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum

Mardirosian

Lascano

Ferrari³

et al. 2015

Enviro Toxic and Chemistry

Self Cite

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“…The intense use of oxygen by active skeletal muscle greatly enhances its generation of ROS via the mitochondria and the vascular endothelium (Ji 1999;Lawler & Song 2002;Jones 2006). The increase in mortality and malformation of aquatic organisms caused by increased generation of ROS (exogenously or endogenously) during development have been well documented (Dennery 2007;Langdon et al 2008;Cole et al 2009;Ferrari, Lascano, Anguiano, Pechen de D' Angelo & Venturino 2009). The increase in mortality and malformation of aquatic organisms caused by increased generation of ROS (exogenously or endogenously) during development have been well documented (Dennery 2007;Langdon et al 2008;Cole et al 2009;Ferrari, Lascano, Anguiano, Pechen de D' Angelo & Venturino 2009).…”

Section: Introductionmentioning

confidence: 99%

Effects of embryonic exposure to α-lipoic acid or ascorbic acid on hatching rate and development of zebrafish (Danio rerio)

Francis

Delgoda

Young

2011

Aquaculture Research

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This study investigates the e¡ects of embryonic exposure to two di¡erent antioxidants on growth and development in ¢sh. Zebra¢sh (Danio rerio) embryos (100 per group) were exposed to lipoic acid (LA, 61 2 mM) or ascorbic acid (AA, 100^200 mM) and the hatching rate, standard lengths (SL) at hatching, development and growth post-hatching monitored. The SLs at hatching were increased (Po0.05) in both antioxidant-exposed groups relative to the controls, with no e¡ect on yolk reserves. This enhanced development persisted up to 15 days post hatching. At hatching, cell proliferation rates (Po0.0005) and basic ¢broblast growth factor (Po0.001), were greater in the antioxidant-exposed ¢sh than in the controls (0 mM antioxidant); no oxidative DNA damage was detected (P40.05). Activity of the endogenous antioxidant enzyme superoxide dismutase was greater (Po0.001) in LA-treated ¢sh than in the controls. The results suggest that embryonic treatment of zeb-ra¢sh with LA or AA during embryogenesis enhanced cell proliferation, leading to increased somatic growth in the larval stages, persisting into the juvenile stage. The ¢ndings support the treatment of embryonic ¢sh with antioxidants for enhanced results in aquaculture.

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“…Endpoints commonly measured include growth14, behavioural abnormalities15, time to metamorphosis1416, deformities17, endocrine disruption81819, induction or suppression of enzymes and endogenous compounds related to oxidative metabolism2021 suppression of immune function2223 and genotoxicity2425. While tadpoles at an early developmental stage are generally regarded as the stage most sensitive to environmental pollution152627, amphibian embryos also show changes in developmental rates and subsequent deformities at metamorphosis as a result of earlier exposure to particular contaminants272829, and thus it is important to consider this stage in any monitoring study.…”

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confidence: 99%

Subtle effects of environmental stress observed in the early life stages of the Common frog, Rana temporaria

Strong

Martin

Jones

et al. 2017

Sci Rep

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Worldwide amphibian populations are declining due to habitat loss, disease and pollution. Vulnerability to environmental contaminants such as pesticides will be dependent on the species, the sensitivity of the ontogenic life stage and hence the timing of exposure and the exposure pathway. Herein we investigated the biochemical tissue ‘fingerprint’ in spawn and early-stage tadpoles of the Common frog, Rana temporaria, using attenuated total reflection-Fourier-transform infrared (ATR-FTIR) spectroscopy with the objective of observing differences in the biochemical constituents of the respective amphibian tissues due to varying water quality in urban and agricultural ponds. Our results demonstrate that levels of stress (marked by biochemical constituents such as glycogen that are involved in compensatory metabolic mechanisms) can be observed in tadpoles present in the pond most impacted by pollution (nutrients and pesticides), but large annual variability masked any inter-site differences in the frog spawn. ATR-FTIR spectroscopy is capable of detecting differences in tadpoles that are present in selected ponds with different levels of environmental perturbation and thus serves as a rapid and cost effective tool in assessing stress-related effects of pollution in a vulnerable class of organism.

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Antioxidant responses to azinphos methyl and carbaryl during the embryonic development of the toad Rhinella (Bufo) arenarum Hensel

Cited by 45 publications

References 60 publications

Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum

Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum

Effects of embryonic exposure to α-lipoic acid or ascorbic acid on hatching rate and development of zebrafish (Danio rerio)

Subtle effects of environmental stress observed in the early life stages of the Common frog, Rana temporaria

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