Antioxidant responses and metal accumulation in tissues of Nile tilapia<i> Oreochromis niloticus </i>under Zn, Cd and Zn + Cd exposures

Fırat, Özgür; Çoğun, Hikmet Yeter; Aslanyavrusu, Sabahattin; Kargın, Ferit

doi:10.1002/jat.1406

Cited by 61 publications

(23 citation statements)

References 52 publications

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“…1). The results of this study are in agreement with our previous study (Fırat et al 2009) which reported an elevation of GSH levels in the gill and liver of Oreochromis niloticus in response to single and combined Zn and Cd exposure for 7 and 28 days, especially at the higher concentrations. GSH is considered a first line of cellular defence against metals by chelating and detoxifying them, scavenging oxyradicals and participating in detoxification reactions catalyzed by glutathione peroxidases (Sies 1999).…”

Section: Gsh Levelsupporting

confidence: 95%

Response of Cyprinus carpio to copper exposure: alterations in reduced glutathione, catalase and proteins electrophoretic patterns

Fırat

Kargın

2010

Fish Physiol Biochem

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This study was conducted to characterize the alterations in reduced glutathione (GSH) level, catalase (CAT) activity and proteins electrophoretic patterns in response to sublethal copper (Cu) exposure in Cyprinus carpio and to determine whether these responses are related to Cu accumulation in gills, chosen as target tissue. Fish were exposed to 0.1 and 1.0 mg/l Cu for 10 and 20 days. There were increasing level of Cu in the gill with increasing concentrations of metal in the exposure medium, and with increasing duration of exposure. GSH level and CAT activity increased in fish exposed to 1.0 mg/l Cu for both exposure periods, while no change was detected at the lower Cu concentration. Electrophoretic patterns of gill proteins by sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) consist of 25-, 26-, 30-, 44- and 48-kDA medium molecular weight proteins (MMP) for five bands and 64-, 72-, 90- and 101-kDA high molecular weight proteins (HMP) for four bands in both control and treatment groups. The levels of 25-, 26- and 30-kDA MMP and 72- and 90-kDA HMP increased in response to Cu exposure. The present study demonstrated that Cu caused stress in fish gills and an acclimation with induction of GSH, CAT, MMP and HMP, which were important in the protection against metal damage, was observed.

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Section: Gsh Levelsupporting

confidence: 95%

Response of Cyprinus carpio to copper exposure: alterations in reduced glutathione, catalase and proteins electrophoretic patterns

Fırat

Kargın

2010

Fish Physiol Biochem

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“…Accumulation of Cd 2? in fish could stimulate the production of ROS, which then reacts with susceptible biological macromolecules, resulting in changes to LPO and antioxidant defenses (Firat et al 2009). In the present study, ROS levels of gills did not significantly change over various exposure times, but were elevated Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have reported that heavy metals induce ROS in fish, and others have examined antioxidant gene expressions to decrease oxidative damage (Firat et al 2009;Cao et al 2010). Such defense mechanisms have been proposed as Fig.…”

Section: Discussionmentioning

confidence: 99%

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Anti-oxidative functions of mt2 and smtB mRNA expression in the gills and brain of zebrafish (Danio rerio) upon cadmium exposure

Shu

2016

Fish Physiol Biochem

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There were not any past studies about metallothionein isoforms (smtB and mt2) having anti-oxidative functions on zebrafish after Cd exposure. On the other hand, the anti-oxidative enzymatic factors such as superoxide dismutase (sod), glutathione peroxidase (gpx1a), and catalase (cat) are used as references to investigate whether the smtB and mt2 have anti-oxidative responses on the gills and brain of zebrafish after 1-6 h of 0 and 1.78 μM Cd exposure. The anti-oxidative system such as sod, cat, and gpx1a mRNA expressions demonstrated a cascade response upon Cd-induced oxidative stress in the present study. Interestingly, the smtB mRNA expression levels increased by 3.2- to 6.1-fold, and mt2 raised by 4.1- to 11.3-fold in gills at 1 and 3 h after exposure to Cd, respectively. On the other hand, the smtB mRNA levels increased by 10.6- to 58.6-fold, but mt2 mRNA levels increased by 2.3- to 11.1-fold in brain at 1 and 3 h after exposure to Cd, respectively. In addition, both tissues showed increased apoptosis levels at 3 h, and recovery after 6 h of Cd exposure. From the results, we suggest that both mt2 and smtB play a role in anti-oxidation responses within 6 h after exposure to Cd. In conclusion, the smtB mRNA levels have a higher response than mt2 in the brain, but both mRNA expressions appear to have a similar pattern in the gill. We suggest that smtB plays an important role to defend oxidative stress in the brain of adult zebrafish upon acute Cd exposure.

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“…Normal homoeotasis status requires a balance between prooxidant and antioxidant systems. Exceeding the defensive capability of antioxidant systems can induce adverse eff ects (van der Schalie et al, 2004;Firat et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Physiological condition status and muscle‐based biomarkers in rainbow trout (Oncorhynchus mykiss), after long‐term exposure to carbamazepine

Li¹,

Žlábek²,

Velíšek³

et al. 2009

J of Applied Toxicology

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Recently, residual pharmaceuticals are generally recognized as relevant sources of aquatic environmental pollutants. However, the toxicological effects of these contaminants have not been adequately researched. In this study, the chronic toxic effects of carbamazepine (CBZ), an anticonvulsant pharmaceutical commonly present in surface and ground water, on physiological condition status and muscle-based biomarkers of rainbow trout were investigated. Fish were exposed at sublethal concentrations of CBZ (1.0 microg l(-1), 0.2 mg l(-1) and 2.0 mg l(-1)) for 42 days. Compared with the control, there was a significant lower (P < 0.05) condition factor in fish exposed at the highest concentration of CBZ (2.0 mg l(-1)), but the hepatosomatic indices in all groups were not significant changes. At lower CBZ concentration (1.0 microg l(-1), 0.2 mg l(-1)), the antioxidant enzyme activities were induced slightly, except catalase, while at the highest concentration (2.0 mg l(-1)) oxidative stress was apparent as reflected by the significant higher lipid peroxidation and protein carbonyls in the fish muscle, associated with a significant inhibition of antioxidant enzymes activities. Moreover, energy metabolic parameters (RNA-DNA ratio, Na(+)-K(+)-ATPase) were significantly inhibited in muscle of the fish exposed at the highest concentration (2.0 mg l(-1)), compared with the control. In short, CBZ-induced physiological and biochemical responses in fish were reflected in parameters measured in this study, which suggest that these biomarkers could be used as potential indicators for monitoring residual pharmaceuticals present in aquatic environment.

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Antioxidant responses and metal accumulation in tissues of Nile tilapia Oreochromis niloticus under Zn, Cd and Zn + Cd exposures

Cited by 61 publications

References 52 publications

Response of Cyprinus carpio to copper exposure: alterations in reduced glutathione, catalase and proteins electrophoretic patterns

Response of Cyprinus carpio to copper exposure: alterations in reduced glutathione, catalase and proteins electrophoretic patterns

Anti-oxidative functions of mt2 and smtB mRNA expression in the gills and brain of zebrafish (Danio rerio) upon cadmium exposure

Physiological condition status and muscle‐based biomarkers in rainbow trout (Oncorhynchus mykiss), after long‐term exposure to carbamazepine

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