Antioxidant responses after microcystin exposure in gills of an estuarine crab species pre-treated with vitamin E

Pinho, Grasiela Lopes Leães; Rosa, C. Moura da; Maciel, Fábio Everton; Bianchini, Adalto; Yúnes, João; Proença, Luís Antônio de Oliveira; Monserrat, José M.

doi:10.1016/j.ecoenv.2004.12.014

Cited by 24 publications

(24 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several studies have documented the protective effects of some antioxidants against the toxic action of MCs from cy‐anobacterial cells when administered to rodents before the toxins [33,34], but research with aquatic organisms is still very scarce [17,18]. The present study showed, to our knowledge for the first time, the effects of NAC dietary supplementation on the oxidative stress status in different organs of tilapia (O. niloticus) exposed to MC‐LR from Microcystis cyanobacterial cells.…”

Section: Discussionmentioning

confidence: 52%

Effects of dietary N‐acetylcysteine on the oxidative stress induced in tilapia (Oreochromis Niloticus) exposed to a microcystin‐producing cyanobacterial water bloom

Puerto

Prieto

Pichardo

et al. 2009

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Fish can be exposed to toxic cyanobacterial cells in natural waters and fish farms and suffer from oxidative damage. The present study investigates the effects of N-acetylcysteine (NAC), a glutathione (GSH) precursor, on the oxidative stress induced by Microcystis cyanobacterial cells containing microcystins (MCs) in tilapia fish (Oreochromis niloticus). Variation in lipid peroxidation (LPO) levels, carbonyl group content, reduced glutathione to oxidized glutathione ratio (GSH:GSSG), and catalase (Enzyme Commission [EC] 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1), glutathione reductase (GR; EC 1.8.1.7), glutathione peroxidase (GPx; EC 1.11.1.9), and glutathione S-transferase (EC 2.5.1.18) activities in liver and kidney of tilapia exposed to a single oral dose of 120 microg MC-LR (with leucine [L] and arginine [R])/fish and killed in 24 h were investigated in the absence and presence of 20.0, 44.0, and 96.8 mg NAC/fish/d. Results showed a protective role of NAC, depending on the dose and the biomarker considered. The increase in LPO (1.9- and 1.4-fold in liver and kidney, respectively) and the decreased protein content and GSH: GSSG in the liver induced by MCs were recovered mainly by the lower doses of NAC employed. Antioxidant enzyme activities increased (range, 1.4- to 1.7-fold) by MCs also were ameliorated by NAC, although the highest level used induced significant alteration of some enzymatic activities, such as SOD, GPx, and GR. Thus, NAC can be considered to be a useful chemoprotectant that reduces hepatic and renal oxidative stress in the prophylaxis and treatment of MC-related intoxications in fish when careful attention is given to its application dose because of its own pro-oxidant activity, as shown in the present study at 96.8 mg NAC/fish/d.

show abstract

Section: Discussionmentioning

confidence: 52%

Effects of dietary N‐acetylcysteine on the oxidative stress induced in tilapia (Oreochromis Niloticus) exposed to a microcystin‐producing cyanobacterial water bloom

Puerto

Prieto

Pichardo

et al. 2009

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…For example, it decreases lipid peroxidation, suppresses ALT leakage, and inhibits GST activity induced by MC-LR (Gehringer et al, 2003). However, it is not effective in inhibiting MC-LR-induced apoptosis, necrosis, and inflammation as assessed by histology (Gehringer et al, 2003; Pinho et al, 2005; Prieto et al, 2009) suggesting that dietary supplements with only antioxidant activity offer limited protection against MC-LR-induced liver injury. In comparison, SFN is a versatile agent showing in this study that SFN offers not only antioxidant activity but also anti-inflammatory and anti-apoptotic functions.…”

Section: Discussionmentioning

confidence: 99%

Sulforaphane prevents microcystin-LR-induced oxidative damage and apoptosis in BALB/c mice

Sun

Liu

et al. 2011

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

Microcystins (MCs), the products of blooming algae Microcystis, are waterborne environmental toxins that have been implicated in the development of liver cancer, necrosis, and even fatal intrahepatic bleeding. Alternative protective approaches in addition to complete removal of MCs in drinking water are urgently needed. In our previous work, we found that sulforaphane (SFN) protects against microcystin-LR (MC-LR)-induced cytotoxicity by activating the NF-E2-related factor 2 (Nrf2)-mediated defensive response in human hepatoma (HepG2) and NIH 3T3 cells. The purpose of this study was to investigate and confirm efficacy the SFN-induced multi-mechanistic defense system against MC-induced hepatotoxicity in an animal model. We report that SFN protected against MC-LR-induced liver damage and animal death at a nontoxic and physiologically relevant dose in BALB/c mice. The protection by SFN included activities of anti-cytochrome P450 induction, anti-oxidation, anti-inflammation, and anti-apoptosis. Our results suggest that SFN may protect mice against MC-induced hepatotoxicity. This raises the possibility of a similar protective effect in human populations, particularly in developing countries where freshwaters are polluted by blooming algae.

show abstract

“…In this review, Table 1 [31,32,33,35,36,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65] shows the various chemoprotectants found in the scientific literature that have been studied with this application, with an indication of the experimental model used (mainly in vivo models), doses or concentration of MCs or the cyanobacterial extract containing MCs which induced damage or intoxication, and the doses of chemoprotectant assayed with the most relevant effects observed against MCs. The majority of the studies have been carried out in mammals (mice), although recently more investigations have been performed on aquatic organisms.…”

Section: Microcystinsmentioning

confidence: 99%

“…Moreover, the effects were evaluated by oral exposure (immersion or diet), more relevant for the aquatic environment. Pinho et al [60] were the first authors to evaluate the effect of vitamin E in antioxidant responses in crabs intoxicated with MC-LR. This study showed that vitamin E pretreatment of intoxicated crabs attenuated the increase in the activity of GST, compared to animals exposed to the toxin only.…”

Section: Microcystinsmentioning

confidence: 99%

Potential Use of Chemoprotectants against the Toxic Effects of Cyanotoxins: A Review

Guzmán-Guillén

Puerto

Gutiérrez-Praena

et al. 2017

Toxins

View full text Add to dashboard Cite

Cyanobacterial toxins, particularly microcystins (MCs) and cylindrospermopsin (CYN), are responsible for toxic effects in humans and wildlife. In order to counteract or prevent their toxicity, various strategies have been followed, such as the potential application of chemoprotectants. A review of the main substances evaluated for this aim, as well as the doses and their influence on cyanotoxin-induced toxicity, has been performed. A search of the literature shows that research on MCs is much more abundant than research on CYN. Among chemoprotectants, antioxidant compounds are the most extensively studied, probably because it is well known that oxidative stress is one of the toxic mechanisms common to both toxins. In this group, vitamin E seems to have the strongest protectant effect for both cyanotoxins. Transport inhibitors have also been studied in the case of MCs, as CYN cellular uptake is not yet fully elucidated. Further research is needed because systematic studies are lacking. Moreover, more realistic exposure scenarios, including cyanotoxin mixtures and the concomitant use of chemoprotectants, should be considered.

show abstract

Antioxidant responses after microcystin exposure in gills of an estuarine crab species pre-treated with vitamin E

Cited by 24 publications

References 15 publications

Effects of dietary N‐acetylcysteine on the oxidative stress induced in tilapia (Oreochromis Niloticus) exposed to a microcystin‐producing cyanobacterial water bloom

Effects of dietary N‐acetylcysteine on the oxidative stress induced in tilapia (Oreochromis Niloticus) exposed to a microcystin‐producing cyanobacterial water bloom

Sulforaphane prevents microcystin-LR-induced oxidative damage and apoptosis in BALB/c mice

Potential Use of Chemoprotectants against the Toxic Effects of Cyanotoxins: A Review

Contact Info

Product

Resources

About