Antioxidant response of a novel Streptomyces sp. M3004 isolated from legume rhizosphere to H2O2 and paraquat

IŞIK, Kübra; Kayalı, Hülya Ayar; Şahi̇n, Nevzat; Gündoğdu, Engin; Tarhan, Leman

doi:10.1016/j.procbio.2006.08.013

Cited by 14 publications

(3 citation statements)

References 32 publications

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“…During dechlorination, the early step of the degradation of chloroacetanilide herbicides, ROS can be produced (Xu et al ., ; Fuentes et al ., ). Other classes of herbicides, such as bipyridyliums and synthetic auxins, could induce oxidative stress due to blockade of electron flow through the electron transport chain and directly or indirectly affect the structure and function of membranes (Işık et al ., ; Lü et al ., ). Therefore, we conclude that oxidative stress induced by atrazine may be due to imbalance of redox potential in bacterial cells, which leads to bacterial metabolic disorder.…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress response in two representative bacteria exposed to atrazine

Zhang

Meng

Wang

et al. 2012

FEMS Microbiol Lett

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Bacteria are present extensively in the environment. Investigation of their antioxidant properties will be useful for further study on atrazine stress tolerance of bacteria and the defense mechanism of antioxidant enzymes against atrazine or other triazine herbicides. Superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and total antioxidant capacity (T-AOC) from one Gram-negative representative strain Escherichia coli K12 and one Gram-positive representative strain Bacillus subtilis B19, respectively, were tested for response to atrazine stress. The results indicated that SOD, CAT, GST and T-AOC were induced upon exposure to atrazine. The growth of two bacteria was better in the absence than in the presence of atrazine, indicating that atrazine can decrease bacterial growth. The changes of enzyme activities indicate the presence of oxidative stress. Oxidative stress induced by atrazine may be due to imbalance of redox potential in bacterial cells, which leads to bacterial metabolic disorder.

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Section: Discussionmentioning

confidence: 99%

Oxidative stress response in two representative bacteria exposed to atrazine

Zhang

Meng

Wang

et al. 2012

FEMS Microbiol Lett

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“…M3004 and E . coli K-12 [49,50]. The redox-cycling compound paraquat generates O 2 - that is dismuted into H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

Long-chain flavodoxin FldX1 improves Paraburkholderia xenovorans LB400 tolerance to oxidative stress caused by paraquat and H2O2

et al. 2019

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Flavodoxins are small electron transfer proteins containing flavin mononucleotide (FMN) as a prosthetic group, which play an important role during oxidative stress or iron limitation. The aims of this study were the identification and characterization of flavodoxins in the model aromatic-degrader Paraburkholderia xenovorans LB400 and the analyses of their protective effects during oxidative stress induced by paraquat and H 2 O 2 . Two genes (BxeA0278 and BxeB0391) encoding flavodoxins (hereafter referred to as fldX for fl avo d oxin from P . x enovorans ), were identified at the LB400 major and minor chromosome. Genomic context of the flavodoxin-encoding genes showed genes encoding membrane proteins, transporters, and proteins involved in redox processes and biosynthesis of macromolecules. A secondary structure prediction of both LB400 flavodoxins showed the characteristic flavodoxin structure of five ß-sheets intercalated with five α-helices. FldX1 contains a loop intercalated in the fifth β-strand, which indicates that it belongs to the long-chain flavodoxins, whereas FldX2 is a short-chain flavodoxin. A phylogenetic analysis of 73 flavodoxins from 43 bacterial genera revealed eight clusters (I-VIII), while FldX1 and FldX2 grouped separately within a long-chain and a short-chain flavodoxin clades. FldX1 and FldX2 were overexpressed in P . xenovorans . Interestingly, the strain overexpressing the long-chain flavodoxin FldX1 (p2-fldX1) showed a faster growth in glucose than the control strain. The recombinant strain overexpressing the long-chain flavodoxin FldX1 (p2- fldx1 ) exposed to paraquat (20 mM) possessed lower susceptibility to growth inhibition on plates and higher survival in liquid medium than the control strain. The strains overexpressing the flavodoxins FldX1 and FldX2 showed higher survival during exposure to 1 mM paraquat (>95%) than the control strain (68%). Compared to the control strain, strains overexpressing FldX1 and FldX2 showed lower lipid peroxidation (>20%) after exposure to 1 mM paraquat and a lower protein carbonylation (~30%) after exposure to 1 mM H 2 O 2 was observed. During exposure to paraquat, strain p2- fldx1 downregulated the katG4 , hpf , trxB1 and ohr genes (> 2-fold), whereas strain p2- fldx2 upregulated the oxyR and ahpC1 genes (> 2-fold). In conclusion, the flavodoxins FldX1 and FldX2 of P . xenovorans LB400 conferred protection to cells exposed to the oxidizing agents paraquat and H 2 O ...

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“…MDA is an indicator of lipid peroxidation, therefore a positive correlation between the MDA and H 2 O 2 levels could indicate that the latter is a causative agent of oxidative stress [ 41 , 42 , 43 ]. Nevertheless, the two B .…”

Section: Discussionmentioning

confidence: 99%

Bacillus megaterium strains derived from water and soil exhibit differential responses to the herbicide mesotrione

Dobrzanski¹,

Gravina²,

Steckling³

et al. 2018

PLoS ONE

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The intense use of herbicides for weed control in agriculture causes selection pressure on soil microbiota and water ecosystems, possibly resulting in changes to microbial processes, such as biogeochemical cycles. These xenobiotics may increase the production of reactive oxygen species and consequently affect the survival of microorganisms, which need to develop strategies to adapt to these conditions and maintain their ecological functionality. This study analyzed the adaptive responses of bacterial isolates belonging to the same species, originating from two different environments (water and soil), and subjected to selection pressure by herbicides. The effects of herbicide Callisto and its active ingredient, mesotrione, induced different adaptation strategies on the cellular, enzymatic, and structural systems of two Bacillus megaterium isolates obtained from these environments. The lipid saturation patterns observed may have affected membrane permeability in response to this herbicide. Moreover, this may have led to different levels of responses involving superoxide dismutase and catalase activities, and enzyme polymorphisms. Due to these response systems, the strain isolated from water exhibited higher growth rates than did the soil strain, in evaluations made in oligotrophic culture media, which would be more like that found in semi-pristine aquatic environments. The influence of the intracellular oxidizing environments, which changed the mode of degradation of mesotrione in our experimental model and produced different metabolites, can also be observed in soil and water at sites related to agriculture. Since the different metabolites may present different levels of toxicity, we suggest that this fact should be considered in studies on the fate of agrochemicals in different environments.

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Antioxidant response of a novel Streptomyces sp. M3004 isolated from legume rhizosphere to H2O2 and paraquat

Cited by 14 publications

References 32 publications

Oxidative stress response in two representative bacteria exposed to atrazine

Oxidative stress response in two representative bacteria exposed to atrazine

Long-chain flavodoxin FldX1 improves Paraburkholderia xenovorans LB400 tolerance to oxidative stress caused by paraquat and H2O2

Bacillus megaterium strains derived from water and soil exhibit differential responses to the herbicide mesotrione

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