Antioxidant properties of methanolic extracts of the leaves of seven Egyptian Cassia species

El‐Hashash, M. A.; Abd-Elgawad, Mahfouz M. M.; El-Sayed, Mortada; Sabry, Waffa Ahmed; Abdel‐Hameed, El‐Sayed S.; Abdel-Lateef, Ezzat El-Sayed

doi:10.2478/v10007-010-0030-y

Cited by 28 publications

(24 citation statements)

References 14 publications

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“…At 0.2 – 0.5 mg/ml, aqueous extract exhibited the highest reducing power that ranges from (2.69 ± 0.13 – 2.88 ± 0.05), followed by ethanol (2.07 ± 0.02 – 2.83 ± 0.06), acetone (1.91 ± 0.28 – 2.36 ± 0.25) and hexane (0.51 ± 0.18 – 1.06 ± 0.018) when compared with BHT (2.97 ± 0.04 – 3.05 ± 0.09) and ascobic acid (3.03 ± 0.08 – 0.04 ± 0.09) respectively. The observed reducing ability of the plant extracts might be due to the presence of hydrophilic polyphenolic compounds [11,37] These results are in full agreement with the previous studies which reported that the reducing power of plant extracts correlated with the phenolic content [36-38]. The reducing capability increase in the following order: Ascorbic acid < BHT < aqueous < ethanol < acetone < hexane.…”

Section: Resultssupporting

confidence: 89%

Antioxidant and phytochemical properties of Carpobrotus edulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province

Omoruyi

Bradley

Afolayan

2012

BMC Complement Altern Med

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BackgroundCarpobrotus edulis (Mesembryanthemaceae), also known as igcukuma in Xhosa language is a medicinal plant used by the traditional healers to treat common infections in HIV/AIDS patients. Based on this information, we researched on the plant phytoconstituents, as well as its inhibitory effect using aqueous and three different organic solvent extracts in order to justify its therapeutic usage.MethodsAntioxidant activity of the extracts were investigated spectrophotometrically against 1,1- diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) diammonium salt, hydrogen peroxide (H2O2), nitric oxide (NO), and ferric reducing power, Total phenols, flavonoids, flavonols, proanthocyanidins, tannins, alkaloids and saponins were also determined using the standard methods.ResultsQuantitative phytochemical analysis of the four solvent extracts revealed a high percentage of phenolics (55.7 ± 0.404%) in the acetone extract, with appreciable amount of proanthocyanidins (86.9 ± 0.005%) and alkaloids (4.5 ± 0.057%) in the aqueous extract, while tannin (48.9 ± 0.28%) and saponin (4.5 ± 0.262%) were major constituents of the ethanol extract. Flavonoids (0.12 ± 0.05%) and flavonols (0.12 ± 0.05%) were found at higher level in the hexane extract in comparison with the other extracts. The leaf extracts demonstrated strong hydrogen peroxide scavenging activity, with the exception of water and ethanol extracts. IC50 values of the aqueous and ethanolic extract against DPPH, ABTS, and NO were 0.018 and 0.016; 0.020 and 0.022; 0.05 and 0.023 mg/ml, respectively. The reducing power of the extract was found to be concentration dependent.ConclusionThe inhibitory effect of the extracts on free radicals may justify the traditional use of this plant in the management of common diseases in HIV/AIDs patients in Eastern Cape Province. Overall, both aqueous and ethanol were found to be the best solvents for antioxidant activity in C. edulis leaves.

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Section: Resultssupporting

confidence: 89%

Antioxidant and phytochemical properties of Carpobrotus edulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province

Omoruyi

Bradley

Afolayan

2012

BMC Complement Altern Med

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“…Extract yield of Gleditsia triacanthos L pods prepared by maceration using increasing polarity solvents showed that the polar extracts (crude methanolic extract, n-butanol and aqueous fractions), were found to contain the highest yields. This result was in full agreements with other studies 7,19,21,22 . The yield is a relative parameter and depends on the plant species studied, the part used in the extraction, the drying and storage conditions, the content of each species in secondary metabolites and the nature of the solvent used in the plant extraction as well as the extraction method itself.…”

Section: Total Antioxidant Capacitysupporting

confidence: 93%

Assessment of Antioxidant Potential and phytochemical screening of Phenolic Compounds of Gleditsia triacanthos L Pods

Saidi¹,

Hassiba

Abbassia³

2018

ijmsci

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Objective: The present study aims at the phytochemical screening, the quantification of phenolic compounds of Gleditsia triacanthos L pods (Family Leguminosae) and the assessment of their antioxidant potential by in vitro assays. Subjects and Methods: The pods were extracted with 70% methanol and further partitioned with petroleum ether, chloroform, ethyl acetate, and n-butanol. The residual aqueous fraction has been also recovered. Colorimetric methods using Folin-Ciocalteu reagent, aluminum chloride and Folin-Denis were carried out to estimate total polyphenols, flavonoids and condensed tannins content of extracts. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and total antioxidant capacity (TAC) were used to determine in vitro antioxidant activity. Results: In vitro phytochemical screening for all the extracts was tested and shown positive result for flavonoids, tannins, cardiac glycosides, sterols and terpenes, saponins and alkaloids. However, all the extracts were free of anthraquinones. The strongest activity against radical scavenging of DPPH was found in the ethyl acetate fraction (IC 50 = 16,288 ± 0,299 µg/ml) which contains highest amounts of flavonoids (25.160 ± 0.016 mg CE/g), whereas the chloroform fraction showed an important total antioxidant capacity (750,584 ± 129,793 mg AAE/g) with the highest amount of total polyphenols (131.667 ± 2.055 mg GAE/g). When compared to the other fractions, the aqueous fraction presented the lowest antioxidant activity for the two methods. Conclusion: These data suggest that the pods of Gleditsia triacanthos L can be a good natural source of antioxidants that can be beneficial for food and human health.

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“…Preliminary phytochemical screening of the crude methanolic extract of C. longa rhizomes was carried out to estimate the amount of flavonoids, phenols, tannins, alkaloids, saponins, terpenoids and steroids according to the standard procedures (14,15).…”

Section: Phytochemical Screeningmentioning

confidence: 99%

Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)

et al. 2016

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The present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a hepatoma cell line. The methanolic extract was subjected to GC-MS analysis to identify the volatile constituents and the other part of the same extract was subjected to liquid column chromatographic separation to isolate curcumin. The inhibition of cell growth in the hepatoma cell line and the cytopathological changes were studied. GC-MS analysis showed the presence of fifty compounds in the methanolic extract of C. longa. The major compounds were ar-turmerone (20.50 %), β-sesquiphellandrene (5.20 %) and curcumenol (5.11 %). Curcumin was identified using IR, 1H and 13C NMR. The inhibition of cell growth by curcumin (IC50 = 41.69 ± 2.87 μg mL-1) was much more effective than that of methanolic extract (IC50 = 196.12 ± 5.25 μg mL-1). Degenerative and apoptotic changes were more evident in curcumin- treated hepatoma cells than in those treated with the methanol extract. Antitumor potential of the methanolic extract may be attributed to the presence of sesquiterpenes and phenolic constituents including curcumin (0.051 %, 511.39 μg g-1 dried methanol extract) in C. longa rhizomes.

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Antioxidant properties of methanolic extracts of the leaves of seven Egyptian Cassia species

Cited by 28 publications

References 14 publications

Antioxidant and phytochemical properties of Carpobrotus edulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province

Antioxidant and phytochemical properties of Carpobrotus edulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province

Assessment of Antioxidant Potential and phytochemical screening of Phenolic Compounds of Gleditsia triacanthos L Pods

Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)

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