Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)

Abdel-Lateef, Ezzat El-Sayed; Mahmoud, Faten S.; Hammam, Olfat; El‐Ahwany, Eman; El-Wakil, Eman Ahmed; Kandil, Sherihan; Taleb, Hoda Abu; El-Sayed, Mortada; Hassenein, Hanaa Ismail

doi:10.1515/acph-2016-0028

Cited by 71 publications

(37 citation statements)

References 24 publications

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“…This suggests possible embryotoxic effect of methanol extract of C. longa at higher concentrations. curcumin has been experimentally reported as the most active and abundant compound present in Curcuma longa [45]. Dose-dependent toxicity effects of curcumin expose to zebrafish was previously observed and the results showed mortality in embryos at third day of incubation at a concentration of 7.5μM of curcumin and producing deformities in zebrafish larvae [46], which is similar to what is reported in this study.…”

Section: Figure 5: Effects Of Curcuma Longa Extract On Zebrafish Larvsupporting

confidence: 90%

Phytochemical evaluation, Embryotoxicity and Teratogenic effects of Curcuma longa extract in Zebrafish (Danio rerio)

Alafiatayo

Song

Ahmad

et al. 2019

Preprint

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Curcuma longa L. is a rhizome plant often used as traditional medicinal preparations in Southeast Asia. The dried powder is commonly known as cure-all herbal medicine with a wider spectrum of pharmaceutical activities. In spite of the widely reported therapeutic applications of C. longa, research on its safety and teratogenic effects on zebrafish embryos and larvae is still limited. Hence, this research was aimed to assess the toxicity of C. longa extract on zebrafish. Using a reflux flask, methanol extract of C. longa was extracted and the identification and quantification of total flavonoids were carried out with HPLC. Twelve fertilized embryos were selected to test the embryotoxicity and teratogenicity at different concentration points. The embryos were exposed to the extract in the E3M medium while the control was only exposed to E3M and different developmental endpoints were recorded with the therapeutic index calculated using the ratio of LC50/EC50. C. longa extract was detected to be highly rich in flavonoids with catechin, epicatechin and naringenin as the 3 most abundant with concentrations of 3,531.34, 688.70 and 523.83μg/mL respectively. The toxicity effects were discovered to be dose-dependent at dosage above 62.50μg/mL, while at 125.0μg/mL, mortality of embryos was observed and physical body deformities of larvae was recorded among the hatched embryos at higher concentrations. Teratogenic effect of the extract was severe at higher concentrations producing physical body deformities such as kink tail, bend trunk, enlarged yolk sac edema. Finally, the Therapeutic Index (TI) values calculated were approximately same for different concentration points tested. Overall, the result revealed that plants having therapeutic potential could also pose threats when consumed at higher doses especially on the embryos. Therefore, detailed toxicity analysis should be carried out on medicinal plants to ascertain their safety on the embryos and its development. Abbreviations:DMSO, dimethyl sulfoxide; HPLC, high performance liquid chromatography; OECD, organisation for economic cooperation and development; FET, fish embryo acute toxicity test; hpf, hour of post fertilization.

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Section: Figure 5: Effects Of Curcuma Longa Extract On Zebrafish Larvsupporting

confidence: 90%

Phytochemical evaluation, Embryotoxicity and Teratogenic effects of Curcuma longa extract in Zebrafish (Danio rerio)

Alafiatayo

Song

Ahmad

et al. 2019

Preprint

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“…It has previously been shown that curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadien-3,5-dione], a diphenolic constituent found in the rhizome of the herb Curcuma longa (commonly known as turmeric), possesses multiple pharmaceutical properties, accounting for various medicinal applications, including anticarcinogenic [15,16], anti-human immunodeficiency virus (HIV)-1 [17], antibiofilm formation [18,19], anti-inflammatory [20], anti-Alzheimer's [21], anti-angiogenic [22], antimicrobial [23], antiviral [24], antimutagenic [25], antioxidant [26], and antidiabetic activities [27]. Curcumin has attracted potential therapeutic interest because it has no side effects and acts on a number of biological targets [28,29].…”

Section: Introductionmentioning

confidence: 99%

Curcumin Analogues with Aldose Reductase Inhibitory Activity: Synthesis, Biological Evaluation, and Molecular Docking

2019

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Curcumin, a constituent of Curcuma longa, has shown numerous biological and pharmacological activities, including antidiabetic effects. Here, a novel series of curcumin analogues were synthesized and evaluated for in vitro inhibition of aldose reductase (AR), the first and rate-limiting enzyme of the polyol pathway, which plays a key role in the onset and progression of diabetic complications. Biological activity studies showed that all the curcuminoids exhibited moderate to good AR inhibitory (ARI) activities compared with that of the quercetin standard. Importantly, compounds 8d, 8h, 9c, 9e, and 10g demonstrated promising ARI activities, with the 50% inhibitory concentration (IC50) values of 5.73, 5.95, 5.11, 5.78, and 5.10 µM, respectively. Four other compounds exhibited IC50 values in the range of 6.04–6.18 µM. Methyl and methoxy derivatives showed a remarkable ARI potential compared with that of other substitutions on the aromatic ring. Molecular docking experiments demonstrated that the most active curcuminoid (10g) was able to favorably bind in the active site of the AR enzyme. The potent ARI activities exhibited by the curcuminoids were attributed to their substitution patterns on the aromatic moiety, which may provide novel leads in the development of therapeutics for the treatment of diabetic complications.

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“…Similar to our results, Zhang et al [22,24] also suggested that CL treatment increased the antioxidant defense system activity in experimentally induced diabetic rabbits. However, Amalraj et al [20,21,25] concluded that CL caused a decrease in lipid peroxidation and an increase in antioxidant defense system activity in the CCl 4 -treated rats. Our findings are in accordance with previous reports of other related studies [17,18,23] that have suggested the protective role of CL powder, possibly due to the antioxidative effect of polyphenols and flavonoids present in the roots, that act as strong superoxide radicals and singlet oxygen quenchers.…”

Section: Discussionmentioning

confidence: 97%

Ultrastructural Changes in Hepatocytes and Chemopreventive Effects of Short-Term Administration of Curcuma longa L. against Oxidative Stress-Induced Toxicity: Improvement Mechanisms of Liver Detoxification

Hasan

2020

Evidence-Based Complementary and Alternative Medicine

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The rhizomes of Curcuma longa L. (CL) have been widely used in herbal medicines worldwide. It has been shown to possess prophylactic effects against oxidative stress. However, there is a paucity of information regarding the protective role of CL against oxidative stress in the absence of toxic agents. The aim of the study was to elucidate the antioxidative stress pharmacodynamics of CL. Eighteen 12-week-old Sprague-Dawley rats weighing about 300 ± 25 gm were divided equally into six groups. Four of the groups were supplemented with CL at 100 mg/kg b.w./day orally (P.O.) and labeled as 1st, 3rd, 5th, and 6th day groups. The PCx (positive control) group was given distilled water orally, and the NCx (negative control) group rats were provided with food and water ad libitum. Blood samples were collected, and rats were sacrificed on days 1, 3, 5, and 6 (2 h) posttreatment. The blood was used for oxidative stress enzyme analysis (SOD, GSH-Px, and MDA) and liver (ALT) and kidney (creatinine) function assay, and the liver was dissected for histology. The results revealed that CL exhibited an antioxidative stress effect in the liver and kidneys as indicated by the low levels of ALT and creatinine. In response to antioxidant enzymes, especially that of the 3rd-day treatment group, an increase in SOD and GSH-Px indirectly caused an alleviation of oxidative stress, leading to a much lower level of MDA. It was concluded that treatment with CL at 100 mg/kg b.w./per day for three consecutive days demonstrated the highest efficacy in abating oxidative stress in rats.

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Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)

Cited by 71 publications

References 24 publications

Phytochemical evaluation, Embryotoxicity and Teratogenic effects of Curcuma longa extract in Zebrafish (Danio rerio)

Phytochemical evaluation, Embryotoxicity and Teratogenic effects of Curcuma longa extract in Zebrafish (Danio rerio)

Curcumin Analogues with Aldose Reductase Inhibitory Activity: Synthesis, Biological Evaluation, and Molecular Docking

Ultrastructural Changes in Hepatocytes and Chemopreventive Effects of Short-Term Administration of Curcuma longa L. against Oxidative Stress-Induced Toxicity: Improvement Mechanisms of Liver Detoxification

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