Antioxidant Properties of Marrubium peregrinum L. (Lamiaceae) Essential Oil

Abstract

The antioxidant activity of Marrubium peregrinum essential oil, collected from three different locations [Backo Gradiste - Rimski Sanac (No.1), Novi Knezevac (No.2) and Senta (No.3)] was evaluated as free radical scavenging capacity (RSC), together with inhibition on xanthine-oxidase and effects on lipid peroxidation (LP). RSC was assessed measuring the scavenging activity of the essential oils on 2,2-diphenyl-1-picrylhydrazyl (DPPH•), super oxide anion (O2•−), nitric-oxide (NO•) and hydroxyl (OH•) radicals. The activities of xanthine-oxidase (XOD) was determined by the nitrite method. Effects on LP were evaluated by following the activities of essential oils in the Fe2+/ascorbate induction system. Experimental results indicate that the essential oil of M. peregrinum from Senta (No.3) exhibited the strongest inhibitory effect, as the IC50 value was achieved with the lowest concentration. The same result was obtained in investigation of influence of essential oil on XOD and LP. The chemical profile of essential oil was evaluated by the means of gas chromatography-mass spectrometry (GC-MS). According to the analysis, the most powerful scavenging compounds were sesquiterpene hydrocarbons (β-caryophyllene, bicyclogermacrene and germacrene-D) and oxygenated sesquiterpenes (spathulenol and caryophyllene oxide).

GC-MS Analysis, Antimicrobial, Scavenging Ability and Cytotoxic Activity of Leaves ofSyzygium calophyllifoliumWalp

“…Sesquiterpenoid (33.54 %) compounds dominated in the ethyl acetate extract. Plant extracts that contain sesquiterpene hydrocarbons are reported to have high antimicrobial activity 27 , scavenging 28 and exhibits anti cancer activity as well 29 .…”

Chemical, pharmacological and nutritional quality assessment of black pepper (Piper nigrumL.) seed cultivars

“…The variation of the antioxidant activity of the black pepper oils in both cultivars might have accounted for their chemical constituents. The monoterpene compounds limonene of lemon essential oil and β‐caryophyllene of Marrubium pergrinum essential oil are reported by their strong antioxidant activity (Kaurinovic et al, ; Yang et al, ). β‐caryophyllene was also reported to have strong scavenging activities against hydroxyl radical and superoxide anion (Calleja et al, ).…”

Liposomes as a Tool to Study Lipid Peroxidation

“…Solution of all concentrations, except the most diluted (0.213 μg/mL), have exhibited a stronger protective effect (from 37.02 to 71.32% of inhibition of LP) than BHT (26.15%). The other two essential oils (from Rimski sanac and Novi Knezevac), at higher concentration (from 1.065 to 2.130 μg/mL), have also exhibited more intense protective effect than BHT [71].…”

“…In particular, the largest inhibitory activity was established by the MeOH extracts of M. peregrinum collected from Senta locality. Also, the best results were obtained using solutions of the highest concentrations [72]. Because of all this there was carried out successive extractions of M. peregrinum from all three localities, and for further work 10% extracts are prepared.…”