Antioxidant properties of Lactuca sativa leaf extract involved in the protection of biomolecules

Harsha, S.N.; Mithila, M. V.

doi:10.1016/j.bionut.2013.06.003

Cited by 17 publications

(10 citation statements)

References 38 publications

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“…The DNA damage and oxidative stress conditions are the results of free radical production initiated by imbalance state of antioxidants and oxidants. In our results, transformed plants showed enhanced DNA protection response due to the effect of rol C gene as compared with previous report (Harsha et al 2013 ). The protective effect of extracts on DNA may be attributed to the presence of phenolics and flavonoids.…”

Section: Discussionsupporting

confidence: 75%

Transformation of Lactuca sativa L. with rol C gene results in increased antioxidant potential and enhanced analgesic, anti-inflammatory and antidepressant activities in vivo

et al. 2016

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Lettuce is an important edible crop which possesses various medicinal properties. In this study Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene. Transgene integration and expression was confirmed through PCR and semiquantitative RT-PCR. The transformed extracts were evaluated for their in vitro antioxidant and in vivo analgesic, anti-inflammatory and antidepressant activities in rats. The transformed plants showed 53–98 % increase in total phenolic and 45–58 % increase in total flavonoid contents compared with untransformed plants. Results of total reducing power and total antioxidant capacity exhibited 90–118 and 61–75 % increase in transformed plants, respectively. In contrast to control, DPPH, lipid peroxidation and DNA protection assay showed up to 37, 20 and 50 % enhancement in transformed plants, respectively. The extracts showed similar but significant enhancement behavior in hot plate analgesic and carrageenan-induced hind paw edema test. The transformed extracts showed 72.1 and 78.5 % increase for analgesic and anti-inflammatory activities, respectively. The transformants of rol C gene exhibited prominent antidepressant activity with 64–73 % increase compared with untransformed plants. In conclusion, the present work suggests that transformation with rol C gene can be used to generate lettuce with enhanced medicinally important properties, such as antioxidant, analgesic, anti-inflammatory and antidepressant potential.

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Section: Discussionsupporting

confidence: 75%

Transformation of Lactuca sativa L. with rol C gene results in increased antioxidant potential and enhanced analgesic, anti-inflammatory and antidepressant activities in vivo

et al. 2016

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“…Antioxidant properties of Lactuca sativa leaf extract involved in the protection of biomolecules was as also reported 29 .…”

Section: Anti-oxidant Propertysupporting

confidence: 66%

Lettuce: a promising leafy vegetable with functional properties

Harsha¹,

Mallesha²,

Sharma³

2017

Def. Life. Sc. Jl.

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Lactuca sativa (lettuce) belongs to Asteraceae family important leafy vegetable known for its medicinal properties. The lettuce is a food that should not lack in our meals, we should keep in mind that its low fat and carbohydrate contents and its high contents of water, it provides caloric power of only 13 Kcal /100 gm are very appropriate for weight loss diets. It is rich in minerals, especially potassium that is very necessary to maintain appropriate levels of liquids in the body, together with calcium and phosphorus makes it especially for the correct well being of the bones. It contains selenium, an antioxidant that has a medicinal property in prevention of certain type of cancers, colon, prostrate and lungs. It contains many necessary amino acids for the formation of the proteins, alanine, and necessary for the construction of muscular and nervous tissues, glycine for the correct operation of immunological system. The most of therapeutic properties of this plant is due to sesquiterpene lactones-lactucin, lactucopicrin the most bioactive compound of Lactuca sativa. This review mainly focuses on the pharmacological action of the Lettuce and active constituents for the wide spectrum biological properties, such as anti-oxidant, anti-inflammatory, anti-microbial, analgesic, neuroprotective and sedative properties, etc. Since Lettuce has medicinal therapeutic potential its usage in dietary supplementation can be exploited. Lettuce has also acquired a folk reputation. More clinical studies are needed to further asses the efficiency and safety in therapeutic application.

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“…Separate foliar and root metal plant uptake experiments were performed using three different vegetable species: lettuce, one of the leafy vegetables most frequently self‐grown for human consumption (Harsha et al, 2014); radish, a root vegetable (Goyeneche et al, 2013); and parsley, an herb used to give fragrance to different food products (Doğru and Erat, 2012). …”

Section: Methodsmentioning

confidence: 99%

Lead and Cadmium Phytoavailability and Human Bioaccessibility for Vegetables Exposed to Soil or Atmospheric Pollution by Process Ultrafine Particles

et al. 2014

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When plants are exposed to airborne particles, they can accumulate metals in their edible portions through root or foliar transfer. There is a lack of knowledge on the influence of plant exposure conditions on human bioaccessibility of metals, which is of particular concern with the increase in urban gardening activities. Lettuce, radish, and parsley were exposed to metal-rich ultrafine particles from a recycling factory via field atmospheric fallouts or polluted soil. Total lead (Pb) and cadmium (Cd) concentrations in of the edible plant parts and their human bioaccessibility were measured, and Pb translocation through the plants was studied using Pb isotopic analysis. The Pb and Cd bioaccessibility measured for consumed parts of the different polluted plants was significantly higher for root exposure (70% for Pb and 89% for Cd in lettuce) in comparison to foliar exposure (40% for Pb and 69% for Cd in lettuce). The difference in metal bioaccessibility could be linked to the metal compartmentalization and speciation changes in relation to exposure conditions. Metal nature strongly influences the measured bioaccessibility: Cd presents higher bioaccessibility in comparison to Pb. In the case of foliar exposure, a significant translocation of Pb from leaves toward the roots was observed. To conclude, the type of pollutant and the method of exposure significantly influences the phytoavailability and human bioaccessibility of metals, especially in relation to the contrasting phenomena involved in the rhizosphere and phyllosphere. The conditions of plant exposure must therefore be taken into account for environmental and health risk assessment.

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Antioxidant properties of Lactuca sativa leaf extract involved in the protection of biomolecules

Cited by 17 publications

References 38 publications

Transformation of Lactuca sativa L. with rol C gene results in increased antioxidant potential and enhanced analgesic, anti-inflammatory and antidepressant activities in vivo

Transformation of Lactuca sativa L. with rol C gene results in increased antioxidant potential and enhanced analgesic, anti-inflammatory and antidepressant activities in vivo

Lettuce: a promising leafy vegetable with functional properties

Lead and Cadmium Phytoavailability and Human Bioaccessibility for Vegetables Exposed to Soil or Atmospheric Pollution by Process Ultrafine Particles

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