Antioxidant properties of dimethyl sulfoxide and its viability as a solvent in the evaluation of neuroprotective antioxidants

Sanmartín-Suárez, Carolina; Soto‐Otero, Ramón; Sánchez-Sellero, Inés; Méndez-Álvarez, Estefanı́a

doi:10.1016/j.vascn.2010.10.004

Cited by 104 publications

(86 citation statements)

References 39 publications

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“…While relatively non-toxic, DMSO can alter processes including cellular differentiation, transcription and methylation (Iwatani et al, 2006;Preisler and Giladi, 1975). Of particular relevance to the present study, DMSO can act as an antioxidant by scavenging hydroxyl radicals, but can also act as a pro-oxidant through interactions with protein thiol groups (Sanmartín-Suárez et al, 2011). UV-A-induced oxidative DNA damage largely occurs through production of singlet oxygen, which is not scavenged by DMSO (Wei et al, 1997).…”

Section: Research Articlementioning

confidence: 86%

Activation of the cnidarian oxidative stress response by ultraviolet light, polycyclic aromatic hydrocarbons and crude oil

Tarrant

Reitzel

Kwok

et al. 2014

Journal of Experimental Biology

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Organisms are continuously exposed to reactive chemicals capable of causing oxidative stress and cellular damage. Antioxidant enzymes, such as superoxide dismutases (SODs) and catalases, are present in both prokaryotes and eukaryotes and provide an important means of neutralizing such oxidants. Studies in cnidarians have previously documented the occurrence of antioxidant enzymes (transcript expression, protein expression and/or enzymatic activity), but most of these studies have not been conducted in species with sequenced genomes or included phylogenetic analyses, making it difficult to compare results across species due to uncertainties in the relationships between genes. Through searches of the genome of the sea anemone Nematostella vectensis Stephenson, one catalase gene and six SOD family members were identified, including three copper/zinc-containing SODs (CuZnSODs), two manganesecontaining SODs (MnSODs) and one copper chaperone of SOD (CCS). In 24 h acute toxicity tests, juvenile N. vectensis showed enhanced sensitivity to combinations of ultraviolet radiation (UV) and polycyclic aromatic hydrocarbons (PAHs, specifically pyrene, benzo[a]pyrene and fluoranthene) relative to either stressor alone. Adult N. vectensis exhibited little or no mortality following UV, benzo[a]pyrene or crude oil exposure but exhibited changes in gene expression. Antioxidant enzyme transcripts were both upregulated and downregulated following UV and/or chemical exposure. Expression patterns were most strongly affected by UV exposure but varied between experiments, suggesting that responses vary according to the intensity and duration of exposure. These experiments provide a basis for comparison with other cnidarian taxa and for further studies of the oxidative stress response in N. vectensis.

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Section: Research Articlementioning

confidence: 86%

Activation of the cnidarian oxidative stress response by ultraviolet light, polycyclic aromatic hydrocarbons and crude oil

Tarrant

Reitzel

Kwok

et al. 2014

Journal of Experimental Biology

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“…In our study, flurbiprofen did not show significant effect on TOS at any concentrations for two treatment periods (12 and 24 h). We considered that these constant TOS values were related with free radical scavenger characteristic of DMSO (Repine et al, 1981;Rosenblum, 1983;Sanmartin-Suarez et al, 2011). Total antioxidant values were statistically decreased in some treatments (58.50 and 117 mg/kg treatment groups, 12 h) and these reductions caused oxidative stress at these groups.…”

Section: Discussionmentioning

confidence: 99%

Investigation of flurbiprofen genotoxicity and cytotoxicity in rat bone marrow cells

Timocin

İla

2014

Drug and Chemical Toxicology

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This study was performed to investigate cytogenetic effects of NSAID flurbiprofen which was used as active ingredient in some analgesic, antipyretic and anti-inflammatory drugs. Genotoxic effect of flurbiprofen was investigated using in vivo chromosome aberration (CA) test and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) test. Also, oxidative stress potential of flurbiprofen was determined by measuring total oxidant and antioxidant level which occurred with flurbiprofen treatment in rat peripheral blood. For these purposes, rats were treated with three concentrations of flurbiprofen (29.25, 58.50 and 117 mg/kg, body weight) in single dose at two different treatment periods (12 and 24 h). According to the results, flurbiprofen did not affect chromosome aberrations in rat bone marrow cells with CA test. In RAPD-PCR test, polymorphic bands were unaffected. Also, test substance did not change total oxidant and antioxidant status (except for 58.50 and 117 mg/kg, 12 h) and therefore it did not lead to significant increase on oxidative stress (again except 58.50 and 117 mg/kg, 12 h). However, flurbiprofen reduced to mitotic indexes and these reductions were dose-dependent for 12 h treatment. In summary, flurbiprofen did not show significant genotoxic effect. But it caused cytotoxicity in rat bone marrow cells.

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“…There are a number of antioxidants with respective spectrum for neutralizing reactive oxygen species. DMSO, an important polar aprotic solvent, has antioxidant properties such as reducing lipid peroxidation (34). Catalase is a common enzyme found in nearly all living organisms that are exposed to oxygen, where it catalyzes the decomposition of hydrogen peroxide to water and oxygen (35).…”

Section: Pharmacological Attenuation Of Cytomix-induced Cytotoxicity mentioning

confidence: 99%

Analysis of cytotoxicity induced by proinflammatory cytokines in the human alveolar epithelial cell line A549

et al. 2012

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Antioxidant properties of dimethyl sulfoxide and its viability as a solvent in the evaluation of neuroprotective antioxidants

Cited by 104 publications

References 39 publications

Activation of the cnidarian oxidative stress response by ultraviolet light, polycyclic aromatic hydrocarbons and crude oil

Activation of the cnidarian oxidative stress response by ultraviolet light, polycyclic aromatic hydrocarbons and crude oil

Investigation of flurbiprofen genotoxicity and cytotoxicity in rat bone marrow cells

Analysis of cytotoxicity induced by proinflammatory cytokines in the human alveolar epithelial cell line A549

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