Antioxidant Properties and Digestive Enzyme Inhibitory Activity of the Aqueous Extract from Leafy Stems of <i>Cissus polyantha</i>

Talba, Mahamad Abba; Miaffo, David; Kamani, Sylviane Laure Poualeu; Kim, Albert; Wansi, Sylvie Léa

doi:10.1155/2019/7384532

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…Concentration-dependent inhibition was also observed with acarbose on both α -glucosidase enzyme activity and α -amylase enzyme activity. These results are similar to those of Mahamad et al [ 35 ] who showed that the aqueous extract of Cissus polyantha has a strong inhibitory effect on the activity of α -amylase and α -glucosidase.…”

Section: Discussionsupporting

confidence: 92%

Efficacy of Rytigynia senegalensis Blume on Free Radical Scavenging, Inhibition of α-Amylase and α-Glucosidase Activity, and Blood Glucose Level

Maidadi

Ntchapda

Miaffo

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Rytigynia senegalensis (Rubiaceae) is a plant used in African medicine for the treatment of diabetes. The aim of this study was to evaluate the in vitro antioxidant, enzyme inhibitory, and hypoglycemic effects of Rytigynia senegalensis extract (RSE). The contents of phenols, tannins, and flavonoids were determined by phytochemical screening. 2,2-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenylpicrylhydrazyl (DPPH) were determined to evaluate the free radical scavenging capacity of the RSE. The inhibitory activity of α-amylase and α-glucosidase was evaluated in vitro using the α-amylase and α-glucosidase inhibition methods and in vivo using the sucrose and starch tolerance tests. The glucose tolerance test was performed on normal rats using doses of 50, 100, and 200 mg/kg of RSE. RSE contains total phenols (36.35 mg GAE/g of extract), flavonoids (11.91 mg QE/g of extract), and tannins (13.01 mg CE/g of extract). RSE exhibits significant radical scavenging activity on DPPH and ABTS radicals with an IC50 of 17.51 and 21.89 μg/mL, respectively. RSE showed an inhibitory effect on the activity of α-amylase and α-glucosidase with an IC50 of 308.93 and 354.13 μg/mL, respectively. RSE (100 and 200 mg/kg) caused a significant decrease in area under the curve and postprandial glycemia at 60, 90, and 120 min following the administration of starch or sucrose. Regarding the glucose tolerance test, RSE (100 and 200 mg/kg) significantly reduced postprandial hyperglycemia from the 90th min posttreatment. RSE lowered postprandial hyperglycemia and has antioxidant properties. These effects would be due to the presence of bioactive compounds in the RSE.

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Section: Discussionsupporting

confidence: 92%

Efficacy of Rytigynia senegalensis Blume on Free Radical Scavenging, Inhibition of α-Amylase and α-Glucosidase Activity, and Blood Glucose Level

Maidadi

Ntchapda

Miaffo

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Furthermore, plant extracts with phenolic compounds exert different biological functions [10], such as antifungal [11,12], antibacterial [13,14], antiviral [15], and anti-inflammatory activities [16]. Another aspect to be considered is the benefit for human health, because phenolic compounds prevent coronary heart diseases [17], have cytotoxic effects [18], have been used in traditional medicine for the treatment of diabetes [19], possess inhibitory activities of enzymes related to Alzheimer`s disease [20] and anti-obesity activities [21]. Exposure of plants to abiotic stress affects plant secondary metabolism and influences the polyphenolic composition and therefore antioxidant properties [22].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Antifungal Activities and Characterization of Phenolic Compounds Using HPLC- MS in Empetrum rubrum Vahl ex Willd.

Schneider,

González-Reyes,

Vergara

et al. 2023

Preprint

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In searching for compounds with antioxidant and antifungal activity, our study focused on the subshrub species Empetrum rubrum Vahl ex Willd. (Ericaceae), commonly known as Brecillo or Murtilla de Magallanes. We measured the antioxidant activity of its methanolic extract (MEE) obtained from the aerial parts (leaves and stems). The antioxidant activity of MEE was evaluated in vitro by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) cationic radical. Results were expressed in gallic acid and Trolox equivalents for DPPH and ABTS assays, respectively. The antioxidant activities, for the DPPH and ABTS assays, were also calculated as an inhibitory concentration of extract needed to inhibit 50% of the absorbance (IC50 value). The results of IC50 values measured by the DPPH and ABTS methods were 0.4145 mg mL-1 and 0.1081 mg mL-1, respectively. In relation to the antioxidant activity, the total phenolic content (TPC) in MEE was determined by the Folin-Ciocalteu method, and the presence of secondary metabolites groups, including phenolic compounds, was determined by using different standardized test procedures. Phenolic compounds contained in leaves, stems, and fruits of E. rubrum were determined qualitatively by means of high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) analysis. The antifungal activity of MEE obtained from aerial parts of E. rubrum was tested against Rhizoctonia solani. In vitro, mycelial growth of this fungus was reduced from 90% to 100% in the presence of MEE.

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Glucose, lipid and oxidative stress lowering activity of the aqueous extract from leafy stems of Cissus polyantha Gilg & Brandt in dexamethasone-induced hyperglycemia in rats

Mahamad

Miaffo

Kamani

et al. 2020

J Diabetes Metab Disord

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Background Diabetes mellitus is a metabolic disorder characterised by chronic hyperglycemia. The present research work aimed to evaluate the hypoglycaemic, hypolipidemic and antioxidant effects of leafy stems of Cissus polyantha Gilg & Brandt in insulin resistant rats. Methods The oral glucose tolerance test (OGTT) was performed in normal rats. Hyperglycemia was induced for 8 days by a daily subcutaneous injection of dexamethasone (1 mg/kg) one hour after pretreatment of animals with metformin (40 mg/kg) and C. polyantha extract (111, 222 and 444 mg/kg). Body weight, blood glucose, insulin level, lipid profile, insulin biomarkers, cardiovascular indices and oxidative stress biomarkers were evaluated. Results For OGTT, the extract (444 mg/kg) produced a significant drop in blood sugar at the 60th (p < 0.01), 90th (p < 0.01) and 120th min (p < 0.05). Morever, the extract at doses of 222 and 111 mg/kg significantly reduced blood sugar at the 60th (p < 0.01) and 90th min (p < 0.05) respectively. Otherwise, C. polyantha (444 and 222 mg/kg) significantly (p < 0.001) increased body weight and decreased blood sugar on the 4th and 8th days of treatment in insulin resistant rats. The extract also significantly decreased (p < 0.001) serum insulin level, hyperlipidemia, insulin resistance index and cardiovascular indices, and increased gluthathione level, and superoxide dismutase and catalase activity. Conclusion The aqueous extract of Cissus polyantha leafy stems (AECPLS) possess hypoglycemic, hypolipidemic and antioxidant activities that could justify its use in traditional medicine for the prevention and treatment of diabetes mellitus and its complications.

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Antioxidant Properties and Digestive Enzyme Inhibitory Activity of the Aqueous Extract from Leafy Stems of Cissus polyantha

Cited by 6 publications

References 23 publications

Efficacy of Rytigynia senegalensis Blume on Free Radical Scavenging, Inhibition of α-Amylase and α-Glucosidase Activity, and Blood Glucose Level

Efficacy of Rytigynia senegalensis Blume on Free Radical Scavenging, Inhibition of α-Amylase and α-Glucosidase Activity, and Blood Glucose Level

Antioxidant and Antifungal Activities and Characterization of Phenolic Compounds Using HPLC- MS in Empetrum rubrum Vahl ex Willd.

Glucose, lipid and oxidative stress lowering activity of the aqueous extract from leafy stems of Cissus polyantha Gilg & Brandt in dexamethasone-induced hyperglycemia in rats

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