Antioxidant Potential of Diosmin and Diosmetin against Oxidative Stress in Endothelial Cells

Abstract

Phlebotropic flavonoids, including diosmin and its aglycone diosmetin, are natural polyphenols widely used in the prevention and treatment of chronic venous insufficiency (CVI). As oxidative stress plays an important role in the development of pathophysiology of the cardiovascular system, the study aimed to investigate the protective effects of diosmin and diosmetin on hydrogen peroxide (H2O2)-induced oxidative stress in endothelial cells. The cells were pretreated with different concentrations of the flavonoid prior to the H2O2 exposure. The cell viability, the level of intracellular reactive oxygen species (ROS), the activity of cellular antioxidant enzymes—including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase GPx—and the malondialdehyde (MDA) level were assessed. It was found that the H2O2-induced oxidative stress was ameliorated by diosmin/diosmetin in a concentration-dependent manner. The flavonoids restored the activity of cellular antioxidant enzymes and lowered the MDA level upregulated by the H2O2 exposure. These results indicate that diosmin and diosmetin may prevent oxidative stress in endothelial cells; therefore, they may protect against the development and progression of oxidative-stress-related disorders.

LC-ESI-MS/MS-Based Comparative Metabolomic Study, Antioxidant and Antidiabetic Activities of Three Lobelia Species: Molecular Modeling and ADMET Study

“…nicotianifolia alcoholic extract. The superior antioxidant behavior observed in the extracts could be attributed to the presence of diosmin and diosmetin . The ethanol extract of L.…”

“…26−28 The evident presence of flavonoids in both L. chinensis and L. sessilifolia has played an essential role in their antioxidant activity. 29 Zimare et al reported that L. nicotianifolia leaf extracts possess good antibacterial, antioxidant, and anticancer properties. 19 Diosmin, diosmetin, and linarin have been shown to have anti-inflammatory 30,31 and antioxidative 32,33 properties.…”

The Antioxidative Effects of Flavones in Hypertensive Disease

“…The vasodilating and antioxidant effects of flavones could also be explained by their ability to induce antioxidant enzymes such as superoxide dismutase, which breaks down ROS, glutathione peroxidase, which increases GSH levels, and HO-1, which scavenges ROS [82]. Although this has been shown to be ultimately beneficial, overproduction of HO-1 and GSH are also associated with various types of cancer and chemotherapy resistance [83].…”

“…Although this has been shown to be ultimately beneficial, overproduction of HO-1 and GSH are also associated with various types of cancer and chemotherapy resistance [83]. Further research is necessary to determine if flavones play a role in chemotherapy resistance, but the use of diosmetin as a therapeutic agent against oxidative stress has been shown to not affect endothelial cellular metabolism or the stability of endothelial cell membranes at concentrations up to 250 µM [82].…”

Network pharmacology to explore the mechanism of scutellarin in the treatment of brain ischaemia and experimental verification of JAK2/STAT3 signalling pathway

“…Scutellarin (4,5,6-trihydroxyflavone-7-glucuronide) is one of the representative flavone. Numerous studies have shown that many flavonoids have antioxidant activity and can improve patient survival 10 – 12 . Previous studies have shown that compared to edaravone, high-dose scutellarin (100 mg/kg) significantly reduces infarct volume after cerebral ischaemia in rats 13 .…”