Antioxidant peptides from protein hydrolysate of skipjack tuna milt: Purification, identification, and cytoprotection on H2O2 damaged human umbilical vein endothelial cells

Novel Antioxidant Collagen Peptides of Siberian Sturgeon (Acipenserbaerii) Cartilages: The Preparation, Characterization, and Cytoprotection of H2O2-Damaged Human Umbilical Vein Endothelial Cells (HUVECs)

“…Figure 5 A shows that the DPPH· scavenging rates of SCP8, SCP10, and SCP11 were 77.03 ± 2.08%, 80.09 ± 2.15%, and 71.1 ± 2.14%, respectively, which were significantly higher than those of ten other isolated collagen APs but still lower than that (95.37 ± 3.25%) of ascorbic acid. In addition, the half clearance concentrations (EC 50 values) of SCP8, SCP10, and SCP11 were 1.27, 1.05, and 1.38 mg/mL, respectively, which were significantly less than those of APs from skipjack tuna milt (GRVPRV: 4.13 mg/mL; AQRPR 1.80 mg/mL) [ 59 ], loach (PSYV: 17.0 mg/mL) [ 60 ], Antarctic krill (NVPDM: 4.88 mg/mL; NGPDPRPSQQ: 7.05 mg/mL; TFPIYDPQ: 2.15 mg/mL) [ 61 ], and hairtail muscle (QNDER: 4.95 mg/mL) [ 62 ].…”

“…Figure 5 B showed that the HO· scavenging rates of SCP8, SCP10, and SCP11 were 81.94 ± 1.05%, 84.11 ± 1.82%, and 76.78 ± 1.92%, respectively, which were significantly higher than those of ten other isolated collagen APs but still lower than that (94.84 ± 2.79%) of ascorbic acid. The EC 50 values of SCP8, SCP10, and SCP11 on HO· were 1.16, 0.97, and 1.63 mg/mL, respectively, which were significantly less than those of APs from skipjack tuna milts (GRVPRV: 5.78 mg/mL; AQRPR 2.80 mg/mL) [ 59 ] and roes (SGE: 2.76 mg/mL; QAEP: 2.10 mg/mL) [ 48 ], miiuy croaker muscle (NFWWP: 2.39 mg/mL; YFLWP: 2.47 mg/mL) [ 63 ], Antarctic krill (NVPDM: 1.84 mg/mL; NWDDMRIVAV: 2.61 mg/mL) [ 61 ], Misgurnus anguillicaudatus (PSYV: 2.64 mg/mL) [ 60 ], and grass carp skin (VGGRP: 2.06 mg/mL; PYSFK: 2.28 mg/mL) [ 64 ]. The present results suggested that SCP8, SCP10, and SCP11 could effectively scavenge excess HO· to inhibit the oxidative stress in cells and biological tissues.…”

Preparation, Characterization, and Cytoprotective Effects on HUVECs of Fourteen Novel Angiotensin-I-Converting Enzyme Inhibitory Peptides From Protein Hydrolysate of Tuna Processing By-Products

“…Among them, skipjack tuna ( Katsuwonus pelamis ) is the most common and abundant species with 3.2 million tons of catches ( 1 ). During the processing of canned tuna, about 50% of skipjack tuna materials are generated as by-products ( 19 – 21 ). On the contrary, several functional molecules, such as collagen/gelatin, unsaturated fatty acid, protease, and polysaccharides, are generated from skipjack tuna by-products and exhibited huge application prospects and various biological activities ( 22 , 23 ).…”

“…On the contrary, several functional molecules, such as collagen/gelatin, unsaturated fatty acid, protease, and polysaccharides, are generated from skipjack tuna by-products and exhibited huge application prospects and various biological activities ( 22 , 23 ). Moreover, some bioactive peptides have been prepared from protein hydrolysates of tuna by-products, such as bone/frame ( 24 ), scale ( 25 ), roe ( 4 ), milt ( 21 ), and head and viscera ( 26 , 27 ). In addition, Chi et al found that protein hydrolysates of tuna dark muscle presented significant radical scavenging and lipid peroxidation inhibition ability ( 28 ).…”

Preparation, Identification, Molecular Docking Study and Protective Function on HUVECs of Novel ACE Inhibitory Peptides from Protein Hydrolysate of Skipjack Tuna Muscle

“…Skipjack tuna ( Katsuwonus pelamis ) is a commercially important marine product worldwide and is considered a high-protein, low-fat, and low-calorie food [ 19 , 20 ]. Functional molecules such as collagens/gelatin, unsaturated fatty acid, protease, and polysaccharides have been generated from skipjack tuna and its by-products and exhibit potential application prospects and various biological activities, e.g., preventing arteriosclerosis, anti-inflammatory, and anticancer, as well as lowering blood cholesterol levels [ 21 , 22 ]. Presently, various bioactive peptides have been found in tuna by-products, such as bone/frame [ 23 , 24 ], scale [ 25 ], roe [ 20 , 26 ], and head and viscera [ 27 , 28 ].…”