Antioxidant enzymes responses to cadmium in radish tissues

Vitória, Ângela Pierre; Lea, Peter J.; Azevedo, Ricardo Antunes

doi:10.1016/s0031-9422(01)00130-3

Cited by 362 publications

(188 citation statements)

References 54 publications

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“…2c, d). The isoenzymic patterns for both leaves and roots are similar to those obtained by Vitoria et al (2001) in a study of cadmium stress in R. sativus tissues. SOD activity also showed an increase in isoenzymes of SOD under excess concentration of Cu (Fig.…”

Section: Discussionsupporting

confidence: 78%

Copper-stress induced alterations in protein profile and antioxidant enzymes activities in the in vitro grown Withania somnifera L.

Rout

Ram

Das

et al. 2013

Physiol Mol Biol Plants

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Withania somnifera L. seedlings were grown in half-strength MS (Murashige and Skoog) basal medium for 4 weeks and then transferred to full-strength MS liquid medium for 3 weeks. The sustainable plants were subcultured in the same medium but with different concentrations (0, 25, 50, 100 and 200 μM) of Cu for 7 and 14 days. The growth parameters (root length, shoot length, leaf length and total number of leaves per plant) showed a declining trend in the treated plants in a concentration dependant manner. Roots and leaves were analyzed for protein profiling and antioxidant enzymes [catalase (CAT, EC 1.11

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Section: Discussionsupporting

confidence: 78%

Copper-stress induced alterations in protein profile and antioxidant enzymes activities in the in vitro grown Withania somnifera L.

Rout

Ram

Das

et al. 2013

Physiol Mol Biol Plants

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“…Similarly, Cd inhibits root growth more promptly than shoot growth (Vitoria et al, 2001). Hence, Cd addition reduced the shoot fresh and dry weight also have been studied previously in different plant species such as wheat (Mane et al, 2010) and barley (Zaltauskaite and Sliumpaite, 2013).…”

Section: Introductionmentioning

confidence: 77%

Alleviating Effect of Exogenous Application of Ascorbic Acid on Growth and Mineral Nutrients in Cadmium Stressed Barley (Hordeum vulgare) Seedlings

Ullah¹,

Javed²,

Wahid³

et al. 2015

IJAB

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An experiment was carried out in sand-filled pots under normal temperature (28±2ºC) to assess the role of exogenously applied ascorbic acid in alleviating the effect of cadmium (Cd) stress on four barley (Hordeum vulgare L.) genotypes (Jau-83, Jau-87, Paidar 91 and Haider 93). After germination, seedlings were exposed to different Cd concentrations (0, 100, 300, 500 and 700 µM CdCl2) along with AsA (200 mg L -1 ) and grown for 15 days. The results suggested that exposure to increased Cd levels caused a significant reduction in growth and mineral nutrients contents of barley seedlings. However, there was a noticeable difference in the effect of Cd on mineral concentrations among genotypes and the difference mainly coincided with differential accumulation of Cd in the shoot and root tissues. When AsA was applied to Cd-stressed plants, it decreased Cd accumulation in shoots and roots and also showed partial reversal of Cd stress effects. It was also observed that at the same Cd concentrations Cd tolerance index of Jau-83 was the highest among the four barley genotypes, indicating that Jau-83 had lower Cd contents in roots may be more tolerant to Cd stress. The application of AsA was effective in reducing the toxicity of increased Cd by reducing the root or shoots Cd contents, as well as by improving the seedling growth attributes and the mineral nutrients in barley.

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“…The unit activity of each SOD isoenzyme was calculated by running a SOD standard from bovine liver (Sigma Chemical Co.). The different types of SOD were differentiated by incubating gels in inhibitors of SOD before staining, such as 2 mM KCN to inhibit Cu/Zn-SOD activity and 3 mM H 2 O 2 to inhibit Cu/Zn-SOD and Fe-SOD activities as described by Vitória et al [37] (Mn-SOD activity is resistant to both inhibitor treatments).…”

Section: Identification Of Isoenzymesmentioning

confidence: 99%

Comparison of ROS formation and antioxidant enzymes in Cleome gynandra (C4) and Cleome spinosa (C3) under drought stress

et al. 2012

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a b s t r a c tDifferences between antioxidant responses to drought in C 3 and C 4 plants are rather scanty. Even, we are not aware of any research on comparative ROS formation and antioxidant enzymes in C 3 and C 4 species differing in carboxylation pathway of same genus which would be useful to prevent other differences in plant metabolism. With this aim, relative shoot growth rate, relative water content and osmotic potential, hydrogen peroxide (H 2 O 2 ) content and NADPH oxidase (NOX) activity, antioxidant defence system (superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR) enzymes and their isoenzymes), CAT1 mRNA level, and lipid peroxidation in seedlings of Cleome spinosa (C 3 ) and Cleome gynandra (C 4 ) species of Cleome genus exposed to drought stress for 5 and 10 day (d) were comparatively investigated. Constitutive levels of antioxidant enzymes (except SOD) were consistently higher in C. spinosa than in C. gynandra under control conditions. CAT1 gene expression in C. spinosa was correlated with CAT activity but CAT1 gene expression in C. gynandra at 10 d did not show this correlation. Drought stress caused an increase in POX, CAT, APX and GR in both species. However, SOD activity was slightly decreased in C. gynandra while it was remained unchanged or increased on 5 and 10 d of stress in C. spinosa, respectively. Parallel to results of malon dialdehyde (MDA), H 2 O 2 content was also remarkably increased in C. spinosa as compared to C. gynandra under drought stress. These results suggest that in C. spinosa, antioxidant defence system was insufficient to suppress the increasing ROS production under stress condition. On the other hand, in C. gynandra, although its induction was lower as compared to C. spinosa, antioxidant system was able to cope with ROS formation under drought stress.

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Antioxidant enzymes responses to cadmium in radish tissues

Cited by 362 publications

References 54 publications

Copper-stress induced alterations in protein profile and antioxidant enzymes activities in the in vitro grown Withania somnifera L.

Copper-stress induced alterations in protein profile and antioxidant enzymes activities in the in vitro grown Withania somnifera L.

Alleviating Effect of Exogenous Application of Ascorbic Acid on Growth and Mineral Nutrients in Cadmium Stressed Barley (Hordeum vulgare) Seedlings

Comparison of ROS formation and antioxidant enzymes in Cleome gynandra (C4) and Cleome spinosa (C3) under drought stress

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