Antioxidant enzymes and the mechanism of the bystander effect induced by ultraviolet C irradiation of A375 human melanoma cells

Ghosh, Rita; Guha, Dipanjan; Bhowmik, Sudipta; Karmakar, Sayantani

doi:10.1016/j.mrgentox.2013.06.022

Cited by 16 publications

(9 citation statements)

References 62 publications

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“…No significant changes in cell viability were observed ( Figure 1B). This is consistent with a previous report that a low dose of UVC exposure for 2 h did not affect the viability of A375 melanoma cells [11]. Then, the UV irradiated cells at doses of 0.4 and 0.8 J/cm 2 for 30 min were considered for the study of cellular oxidants, melanin synthesis, and tyrosinase activity in B6 melanoma cells treated with S. reticulata root extract.…”

supporting

confidence: 90%

The Effects of Salacia reticulata on Anti-Cellular Oxidants and Melanogenesis Inhibition in α-MSH-stimulated and UV Irradiated B16 Melanoma Cells

Suwannalert

Kariya

Suzu

et al. 2014

Natural Product Communications

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The purposes of this study were to investigate the inhibitory effects of Salacia reticulata Tul. root extract on cellular oxidants and melanogenesis in B16 melanoma cells. Cells treated with non-toxic doses of S. reticulata root extract were investigated for their effects on melanogenesis, cellular tyrosinase activity and cellular oxidant scavenging activity. The results indicated that S. reticulata extract inhibited melanin synthesis and tyrosinase activity in α-MSH-induced or UV-irradiated B16 melanoma cells in a dose dependent manner. Additionally, the extract also exhibited anti-cellular oxidants in UV-induced radical melanoma cells. Altogether, these results suggested that S. reticulata root extract has roles in suppression of melanogenesis and oxidant inhibition. S. reticulata root extract may be a potential source for the development of pharmaceutical products for treatment of skin hyperpigmentation disorders.

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supporting

confidence: 90%

The Effects of Salacia reticulata on Anti-Cellular Oxidants and Melanogenesis Inhibition in α-MSH-stimulated and UV Irradiated B16 Melanoma Cells

Suwannalert

Kariya

Suzu

et al. 2014

Natural Product Communications

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“…Melanocytes are located in the basal epidermal layer and in the hair follicles. Generally, UV radiation produces pigment by diametric stimulation of melanocytes [ 7 ]. Melanin pigmentation has been recognized by many factors; the permeation of sunlight is a well recognized source of melanin pigmentation; specifically, UV radiation causes darkening of the skin and/or sunburn.…”

Section: Introductionmentioning

confidence: 99%

Biofunctional Activities ofEquisetum ramosissimumExtract: Protective Effects against Oxidation, Melanoma, and Melanogenesis

Chiu

Shih

et al. 2016

Oxidative Medicine and Cellular Longevity

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Equisetum ramosissimum, a genus of Equisetaceae, is a medicinal plant that can be separated into ethyl acetate (EA), dichloromethane (DM), n-hexane (Hex), methanol (MeOH), and water extracts. EA extract was known to have potent antioxidative properties, reducing power, DPPH scavenging activity, and metal ion chelating activity. This study compared these five extracts in terms of their inhibiting effects on three human malignant melanomas: A375, A375.S2, and A2058. MTT assay presented the notion that both EA and DM extracts inhibited melanoma growth but did not affect the viabilities of normal dermal keratinocytes (HaCaT) or fibroblasts. Western blot analyses showed that both EA and DM extracts induced overexpression of caspase proteins in all three melanomas. To determine their roles in melanogenesis, this study analyzed their in vitro suppressive effects on mushroom tyrosinase. All extracts except for water revealed moderate suppressive effects. None of the extracts affected B16-F10 cells proliferation. EA extract inhibited cellular melanin production whereas DM extract unexpectedly enhanced cellular pigmentation in B16-F10 cells. Data for modulations of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein 1, and tyrosinase-related protein 2 showed that EA extract inhibited protein expression mentioned above whereas DM extract had the opposite effect. Overall, the experiments indicated that the biofunctional activities of EA extract contained in food and cosmetics protect against oxidation, melanoma, and melanin production.

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“…Recently, the defense system of bystander cells responding to harmful signals has received much attention293031. Our present work disclosed that the bystander hepatocyte cells could trigger innate ER stress against the damage responses from irradiated hepatoma cells.…”

Section: Discussionmentioning

confidence: 61%

Protective effect of mild endoplasmic reticulum stress on radiation-induced bystander effects in hepatocyte cells

Xie

Zhang

et al. 2016

Sci Rep

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Radiation-induced bystander effect (RIBE) has important implications for secondary cancer risk assessment during cancer radiotherapy, but the defense and self-protective mechanisms of bystander normal cells are still largely unclear. The present study found that micronuclei (MN) formation could be induced in the non-irradiated HL-7702 hepatocyte cells after being treated with the conditioned medium from irradiated hepatoma HepG2 cells under either normoxia or hypoxia, where the ratio of the yield of bystander MN induction to the yield of radiation-induced MN formation under hypoxia was much higher than that of normoxia. Nonetheless, thapsigargin induced endoplasmic reticulum (ER) stress and dramatically suppressed this bystander response manifested as the decrease of MN and apoptosis inductions. Meanwhile, the interference of BiP gene, a major ER chaperone, amplified the detrimental RIBE. More precisely, thapsigargin provoked ER sensor of PERK to initiate an instantaneous and moderate ER stress thus defensed the hazard form RIBE, while BiP depletion lead to persistently destroyed homeostasis of ER and exacerbated cell injury. These findings provide new insights that the mild ER stress through BiP-PERK-p-eIF2α signaling pathway has a profound role in protecting cellular damage from RIBE and hence may decrease the potential secondary cancer risk after cancer radiotherapy.

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Antioxidant enzymes and the mechanism of the bystander effect induced by ultraviolet C irradiation of A375 human melanoma cells

Cited by 16 publications

References 62 publications

The Effects of Salacia reticulata on Anti-Cellular Oxidants and Melanogenesis Inhibition in α-MSH-stimulated and UV Irradiated B16 Melanoma Cells

The Effects of Salacia reticulata on Anti-Cellular Oxidants and Melanogenesis Inhibition in α-MSH-stimulated and UV Irradiated B16 Melanoma Cells

Biofunctional Activities ofEquisetum ramosissimumExtract: Protective Effects against Oxidation, Melanoma, and Melanogenesis

Protective effect of mild endoplasmic reticulum stress on radiation-induced bystander effects in hepatocyte cells

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