Antioxidant enzyme responses to chilling stress in differentially sensitive inbred maize lines

Hodges, D. Mark; Andrews, Christopher J.; Johnson, Douglas A.; Hamilton, R. I.

doi:10.1093/jxb/48.5.1105

Cited by 158 publications

(102 citation statements)

References 39 publications

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“…In one study (Lee et al, 2007) simultaneous overexpression of Cu/Zn and APX in tall fescue resulted in an increase tolerance to a wide range of abiotic stresses. In another study, comparison of chilling sensitive to a more tolerant maize (Zea mays) line suggested that the reduced activities of APX, catalase, and monodehydroascobate reductase may contribute to lower chilling tolerance at the early stages of development in maize (Hodges et al, 1997). Thus the level of activity of these enzymes before cold stress appeared important for cold tolerance.…”

Section: Antioxidative and Detoxification Proteinsmentioning

confidence: 99%

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Koehler

Wilson²,

Goodpaster

et al. 2012

Plant Physiology

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To gain insight into the molecular basis contributing to overwintering hardiness, a comprehensive proteomic analysis comparing crowns of octoploid strawberry (Fragaria 3 ananassa) cultivars that differ in freezing tolerance was conducted. Four cultivars were examined for freeze tolerance and the most cold-tolerant cultivar ('Jonsok') and least-tolerant cultivar ('Frida') were compared with a goal to reveal how freezing tolerance is achieved in this distinctive overwintering structure and to identify potential cold-tolerance-associated biomarkers. Supported by univariate and multivariate analysis, a total of 63 spots from twodimensional electrophoresis analysis and 135 proteins from label-free quantitative proteomics were identified as significantly differentially expressed in crown tissue from the two strawberry cultivars exposed to 0-, 2-, and 42-d cold treatment. Proteins identified as cold-tolerance-associated included molecular chaperones, antioxidants/detoxifying enzymes, metabolic enzymes, pathogenesis-related proteins, and flavonoid pathway proteins. A number of proteins were newly identified as associated with cold tolerance. Distinctive mechanisms for cold tolerance were characterized for two cultivars. In particular, the 'Frida' cold response emphasized proteins specific to flavonoid biosynthesis, while the more freezing-tolerant 'Jonsok' had a more comprehensive suite of known stress-responsive proteins including those involved in antioxidation, detoxification, and disease resistance. The molecular basis for 'Jonsok'-enhanced cold tolerance can be explained by the constitutive level of a number of proteins that provide a physiological stress-tolerant poise. Strawberry (Fragaria 3 ananassa) cultivation predominates in regions with mild winters. In colder climates, overwintering hardiness is an essential trait for strawberry cultivation. Freezing injury of strawberry plants is one of the greatest factors in reducing crop yield and quality in temperate regions. Winter damage in Norway, for example, on average causes losses of 20%. Thus, production of cultivars with improved freezing hardiness is one of Norway's major objectives for their strawberry breeding programs. Improvement of cold hardiness is desirable for securing economic sustainability of the existing crops, and for expanding the growing regions of temperate fruit crops. Because strawberry is a representative species for the Rosaceae crops (e

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Section: Antioxidative and Detoxification Proteinsmentioning

confidence: 99%

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Koehler

Wilson²,

Goodpaster

et al. 2012

Plant Physiology

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“…Os extratos enzimáticos brutos, usados para as determinações das atividades da superóxido dismutase (SOD), ascorbato peroxidase (APX) e catalase (CAT) foram obtidos a partir da metodologia descrita por Hodges et al (1997), com pequenas adaptações. Amostras de 16 eixos embrionários e 16 endospermas micropilares foram separadamente trituradas, seguido da adição de 2,0 mL do seguinte meio de homogeneização: tampão fosfato 50 mM, pH 7,8 e polivinilpolipirrolidona (PVPP) 1% (p/v).…”

Section: Ensaios Enzimáticosunclassified

Atividade enzimática durante a germinação de sementes de Melanoxylon brauna schott sob diferentes temperaturas

Flores

Borges

Guimarães

et al. 2014

CERNE

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ATIVIDADE ENZIMÁTICA DURANTE A GERMINAÇÃO DE SEMENTES DEMelanoxylon brauna Schott SOB DIFERENTES TEMPERATURAS RESUMO: Objetivou-se, com este trabalho, investigar o efeito da temperatura sobre o metabolismo oxidativo, por meio da atividade das enzimas superóxido dismutase (SOD), ascorbato peroxidase (APX) e catalase (CAT) no eixo embrionário e no endosperma micropilar de sementes de Melanoxylon brauna Schott, durante a germinação. As sementes foram semeadas em rolos de papel germitest umedecidos com água destilada e submetidas às temperaturas constantes de 15, 25, 30 e 40°C, durante 10 dias. As determinações das atividades das enzimas SOD, APX e CAT foram feitas nas sementes colocadas para germinar sob as mesmas condições descritas acima e a cada 12 horas foram retiradas amostras até a protrusão radicular de 50% das sementes ou 168 horas para a temperatura de 15°C. Não foram detectadas atividades das enzimas SOD, APX e CAT no endosperma micropilar nas diferentes temperaturas. No eixo embrionário, as atividades das enzimas SOD e CAT apresentaram maiores alterações ao longo da germinação em 15°C e 40°C, demonstrando que temperaturas estressantes provocam danos oxidativos. A atividade da enzima APX, no eixo embrionário, não foi afetada pela temperatura ao longo da germinação e sua atividade foi extremamente baixa. ENZYMATIC ACTIVITY DURING GERMINATION OF Melanoxylon brauna Schott AT DIFFERENT TEMPERATURESABSTRACT: The objective of this study was to investigate the effect of temperature on the oxidative metabolism through the activity of the enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) in the embryonic axis and micropylar endosperm of Melanoxylon brauna Schott seeds during germination. The seeds were sown in paper rolls germitest moistened with distilled water and subjected to constant temperatures of 15, 25, 30 and 40°C for 10 days. Measurements of the activities of enzymes SOD, APX and CAT were made in the seeds germinated under the same conditions described above, and every 12 hours until the samples were taken root protrusion of 50% of the seeds or 168 hours for the temperature 15°C. There were no activities of the enzymes SOD, APX and CAT in the micropylar endosperm at different temperatures. In the embryonic axis, the activities of the enzymes SOD and CAT showed greater changes over the germination of 15°C and 40°C, demonstrating that temperature stress causes oxidative damage. The APX enzyme activity in the embryonic axis was not affected by temperature during germination and its activity was extremely low.

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“…Catalase (CAT; EC 1.11.1.6) activity was determined according to Hodges et al (1997) method based on hydrogen peroxide decomposition by catalase which is expressed as absorbance decreasing of a reacting sample solution. The reaction mixture contained: 50 mM phosphate buffer (pH 7.3), 30 mM hydrogen peroxide and 0.15 mL of supernatant.…”

Section: Enzymes Activitiesmentioning

confidence: 99%

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

et al. 2012

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Abstract:The seeds of Zea mays L. cv. KWS were exposed to low-temperature plasma (LTP) by using Diffuse Coplanar Surface Barrier Discharge (DCSBD) for 60 and 120 seconds respectively. Growth parameters, anatomy of roots and activity of some enzymes (CAT, G-POX, SOD and DHO) isolated from roots grown from the seeds treated by LTP were evaluated. Our results indicate that LTP treatment of maize seeds affects post-germination growth of seedlings and this effect depends on the duration of LTP treatment. LTP treatment in duration of 60 seconds significantly increased the length, fresh and dry weight of the roots. However, the increase in time of LTP treatment to 120 seconds had inhibitive effect on the studied growth parameters. The activities of all studied antioxidant enzymes significantly increased with the age of maize seedlings in control conditions. On the other hand the application of LTP resulted in small, mostly non significant changes in the activity of antioxidant enzymes. Significant decrease in CAT activity was observed both in 3 and 6-day old maize roots and G-POX activity in 3-day old maize roots grown from seeds exposed to LTP for 60 seconds. A small, significant increase was detected only in SOD activity in 3-day old maize roots grown from seeds treated with LTP for 120 seconds and in 6-day old maize roots treated with LTP for 60 seconds. Significantly higher DHO activity was determined in embryos isolated from seeds treated with LTP for 60 seconds. On the contrary, in roots the DHO activity decreased with the time of LTP treatment. LTP treatment of seeds did not affect the anatomy of maize roots and caused only minor changes in the isoenzyme composition of G-POX and SOD.

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Antioxidant enzyme responses to chilling stress in differentially sensitive inbred maize lines

Cited by 158 publications

References 39 publications

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Atividade enzimática durante a germinação de sementes de Melanoxylon brauna schott sob diferentes temperaturas

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

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