Antioxidant-enriched autologous biogel promoted diabetic wound healing by remodeling inherent posttraumatic inflammatory patterning and restoring compromised microenvironment homeostasis

Abstract

Successful wound healing depends on the reconstruction of proper tissue homeostasis, particularly in the posttraumatic inflammatory tissue microenvironment. Diabetes jeopardizes tissues’ immune homeostasis in cutaneous wounds, causing persistent chronic inflammation and cytokine dysfunction. Previously, we developed an autologous regeneration factor (ARF) technology to extract the cytokine composite from autologous tissue to restore immune homeostasis and promote wound healing. However, treatment efficacy was significantly compromised in diabetic conditions. Therefore, we proposed that a combination of melatonin and ARF, which is beneficial for proper immune homeostasis reconstruction, could be an effective treatment for diabetic wounds. Our research showed that the utilization of melatonin-mediated ARF biogel (AM gel) promoted diabetic wound regeneration at a more rapid healing rate. RNA-seq analysis showed that AM gel treatment could restore more favorable immune tissue homeostasis with unique inflammatory patterning as a result of the diminished intensity of acute and chronic inflammation. Currently, AM gel could be a novel and promising therapeutic strategy for diabetic wounds in clinical practice through favorable immune homeostatic reconstructions in the tissue microenvironment and proper posttraumatic inflammation patterning.

Multifunctional Drugs-Loaded Carbomol Hydrogel Promotes Diabetic Wound Healing via Antimicrobial and Immunoregulation

“…The immune microenvironment plays a crucial role in the process of diabetic wound healing [ 49 , 50 ]. CD86/CD206 serve as indicators for M1 and M2 macrophages, respectively, which are used to evaluate the changes in the immune microenvironment levels after different treatments in diabetic wound healing.…”

Characterization of distinct microbiota associated with androgenetic alopecia patients treated and untreated with platelet‐rich plasma (PRP)

“…PRP was isolated, divided into three parts, pre‐frozen at −80°C for 24 h, and then freeze‐dried using a freeze‐drying machine for 48 h. After freeze‐drying, PRP was stored at −20°C. 3 , 12 , 13 Subjects were treated once a month and then blood was collected again after three months to isolated PRP. The total treatment course was 6 months.…”

Recent advances in regenerative biomaterials

“…At least three aspects should be considered into the design of a wound dressing material—inflammation, proliferation and remodeling [ 315 , 316 ]. For example, inhibiting the generation of pro-inflammatory cytokines is an approach to accelerate wound healing [ 317 , 318 ]. Since ROS could lead to inflammatory responses in the early stage, scavenging ROS has been considered in wound dressing development.…”