Antioxidant effects of the sarsaparilla via scavenging of reactive oxygen species and induction of antioxidant enzymes in human dermal fibroblasts

Park, Gunhyuk; Kim, Tae-mi; Kim, Jeong Hee; Oh, Myung Sook

doi:10.1016/j.etap.2014.06.009

Cited by 12 publications

(7 citation statements)

References 28 publications

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“…This radical scavenging activity determination was performed as previously described by Park et al . . Initially, a 0.2 mM diphenylpicrylhydrazyl (DPPH) solution was dissolved in ethanol and mixed with the same volume (1 : 1) of varying concentrations of 23‐HTA (0–100 μg/ml).…”

Section: Methodsmentioning

confidence: 99%

23‐Hydroxytormentic acid protects human dermal fibroblasts by attenuating UVA‐induced oxidative stress

Youn

Kim²,

Han³

et al. 2017

Photoderm Photoimm Photomed

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Section: Methodsmentioning

confidence: 99%

23‐Hydroxytormentic acid protects human dermal fibroblasts by attenuating UVA‐induced oxidative stress

Youn

Kim²,

Han³

et al. 2017

Photoderm Photoimm Photomed

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“…The cell culture system was established according to previously published methods [10]. The HS68 cell line, human skin fibroblast cells, was obtained from the American Type Culture Collection (ATCC; Rockville, Md., USA).…”

Section: Methodsmentioning

confidence: 99%

Acceleration of Collagen Breakdown by Extracellular Basic pH in Human Dermal Fibroblasts

Park

Kim

et al. 2016

Skin Pharmacol Physiol

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Background/Aims: Wound healing is a complex regeneration process involving the degradation and reassembly of connective tissues and skin layers. Previous studies have shown that pH plays a signiﬁcant role in both the direct and indirect regulation of cellular processes in the wound, which, in turn, affect the wound healing process. However, the effects of pH on the collagen breakdown component of wound healing have yet to be investigated. Therefore, we investigated the induction of accelerated collagen breakdown by pH imbalance in the skin. Methods: Na⁺/H⁺ exchanger and metalloproteinase (MMP)-1 were analyzed spectrophotometrically, and the expression of collagen type-I-alpha-1 (COL1A1) and mitogen-activated protein kinase (MAPK) was measured by Western blotting. Results: Accelerated collagen breakdown induced by extracellular basic pH via the overproduction of reactive oxygen species (ROS) and MAPK signaling was examined in skin fibroblasts and in a three-dimensional human skin equivalent system. Basic pH (>7.50) upregulated MMP-1 and downregulated COL1A1 levels via ROS generation and MAPK signaling pathways. Acidic pH (<6.04) slightly upregulated MMP-1 and slightly downregulated COL1A1 levels via ROS generation and the p38 signaling pathway. Conclusion: Our results indicate that skin pH is an important effector of collagen formation in wound healing. This finding will aid in the development of new pH-targeted therapeutic strategies.

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“…Smilax glabra Roxb, belonging to the family Liliaceae, is a perennial evergreen climbing shrub mainly distributed in China, Japan, Thailand, and so forth [ 4 – 6 ]. This plant is generally consumed as a substitute for tea and sugar to prevent scurvy, and for treating a range of different conditions such as chest ailments, rheumatism, leprosy, impotence, syphilis, and so forth [ 7 ]. Syrup made by prolonged boiling of the leaves of S .…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Chemical Compounds Isolated from the Rhizome ofSmilax glabraon Nitric Oxide and Tumor Necrosis Factor-αProduction in Lipopolysaccharide-Induced RAW264.7 Cell

Zhu

Wang

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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The rhizome of Smilax glabra has been used for a long time as both food and folk medicine in many countries. The present study focused on the active constituents from the rhizome of S. glabra, which possess potential anti-inflammatory activities. As a result, nine known compounds were isolated from the rhizome of S. glabra with the bioassay-guiding, and were identified as syringaresinol (1), lasiodiplodin (2), de-O-methyllasiodiplodin (3), syringic acid (4), 1,4-bis(4-hydroxy-3,5-dimethoxyphenyl)-2,3-bis(hydroxymethyl)-1,4-butanediol (5), lyoniresinol (6), trans-resveratrol (7), trans-caffeic acid methyl ester (8), and dihydrokaempferol (9). Among these compounds, 2 and 3 were isolated for the first time from S. glabra. In addition, the potential anti-inflammatory activities of the isolated compounds were evaluated in vitro in lipopolysaccharide- (LPS-) induced RAW264.7 cells. Results indicated that 4 and 7 showed significant inhibitory effects on NO production of RAW264.7 cells, and 1, 2, 3, and 5 showed moderate suppression effects on induced NO production. 1, 7, and 5 exhibited high inhibitory effects on TNF-α production, with the IC50 values less than 2.3, 4.4, and 16.6 μM, respectively. These findings strongly suggest that compounds 1, 2, 3, 4, 5, 7, and 9 were the potential anti-inflammatory active compositions of S. glabra.

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Antioxidant effects of the sarsaparilla via scavenging of reactive oxygen species and induction of antioxidant enzymes in human dermal fibroblasts

Cited by 12 publications

References 28 publications

23‐Hydroxytormentic acid protects human dermal fibroblasts by attenuating UVA‐induced oxidative stress

23‐Hydroxytormentic acid protects human dermal fibroblasts by attenuating UVA‐induced oxidative stress

Acceleration of Collagen Breakdown by Extracellular Basic pH in Human Dermal Fibroblasts

Inhibitory Effects of Chemical Compounds Isolated from the Rhizome ofSmilax glabraon Nitric Oxide and Tumor Necrosis Factor-αProduction in Lipopolysaccharide-Induced RAW264.7 Cell

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