Antioxidant effects of ryegrass phenolics in lamb liver and plasma

Abstract

Sixteen lambs were divided into two groups and fed two different diets. Eight lambs were stall-fed with a concentrate-based diet (C), and the remaining eight lambs were allowed to graze on Lolium perenne (G). The antioxidant status was measured in the liver and plasma samples before and after solid-phase extraction (SPE) to probe the antioxidant effects that grass phenolic compounds may have conferred onto the animal tissues. The liver and plasma samples from grass-fed lambs displayed a greater antioxidant capacity than the tissues from C lamb group, but only if samples had not been passed through SPE cartridges. Finally, the feed and animal tissues, which had been purified by SPE, were analysed by liquid chromatography combined with mass spectrometry (LC-MS) to identify phenolic compounds present in L. perenne and to evaluate the results from the antioxidant assays. It would appear that the improvement of the antioxidant capacity of lamb liver and plasma from lambs fed ryegrass was not related to the direct transfer of phenolic compounds from grass to the animal tissues.

Effects of adding solid and molten chocolate on the physicochemical, antioxidant, microbiological, and sensory properties of ewe's milk cheese

“…The total concentration of polyphenols in sample extracts was determined according to the Folin–Ciocalteau colorimetric method, as reported by López‐Andrés et al. (). The results were expressed as g gallic acid equivalent (GAE)/kg DM.…”

Effect of the inclusion of fresh lemon pulp in the diet of lactating ewes on the properties of milk and cheese

“…Cheese extracts were prepared, according to the method of Rashidinejad et al (2013) with slight modifications, to measure cheese antioxidant status by the determination of total phenolic compound content and the TEAC assay with the same methods already described for feeds (López-Andrés et al, 2014;Re et al, 1999). To prepare the cheese sample extracts, milled and freeze-dried cheese samples (0.5 g) were homogenised for 30 s and then extracted for 30 min with 25 ml of methanol (95% aqueous solution) containing 1% HCl at 50°C on an orbital shaker at 200 rpm.…”

“…The total concentration of polyphenols in the feed sample extracts was measured using the Folin-Ciocalteau colorimetric method, as described by López-Andrés et al (2014). Briefly, 100 μl of the sample extract was transferred into a 15-ml centrifuge tube; then, 900 μl of distilled water and 500 μl of the Folin-Ciocalteau reagent, diluted to a concentration of 1 N with distilled water, were added.…”

Actividade Antioxidante en Carne de Tenreiros Alimentados con Bagazo de Uva

“…A herba procede de praderías polifitas formadas por unha mestura de raigrás inglés, dactilo, Agrostis spp e trevo branco fundamentalmente, aínda que tamén hai outras especies de menor arraigo inicial que van invadindo as praderías conforme teñen xa uns anos. No caso do raigrás inglés (Lolium perenne) atopáronse nos seus extractos flavonoides glicósidos e ácidos hidroxicinámicos como os polifenois máis abundantes neles (Qawasmeh et al 2012) e intensidade baixas de glicósidos de Kaempferol (López-Andrés et al 2014). Aparecen compostos fenólicos no caso do Dactilo (Dactylis glomerata) (Hauck et al 2014).…”