Antioxidant effects of mycophenolate mofetil in a murine pleurisy model

Dalmarco, Eduardo Monguilhott; Budni, Patrícia; Parisotto, Eduardo Benedetti; Filho, Danilo Wilhelm; Fröde, Tânia Sílvia

doi:10.1016/j.trim.2009.09.005

Cited by 18 publications

(14 citation statements)

References 27 publications

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“…Lung samples were taken and used to evaluate p65 (Nuclear factor kappa B subunit) and p38 [19][20][21].…”

Section: E P T E Dmentioning

confidence: 99%

“…A total of 29 subfractions were produced and eluted with increasing amounts of ethyl acetate (EtOAc) in n-hexane (0.5:9.5, 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1, 10:0) and monitored by TLC (Thin-layer chromatography). The similar fractions (13)(14)(15)(16)(17)(18)(19)(20)(21) were resubmitted to silica gel column chromatography (CC), yielding a further 50 subfractions. Between 40 and 48 subfractions were combined and purified with acetone and identified as scopoletin (6:4 of EtOAc:n-hexane) (118 mg) (Fig.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of the NF-κB and p38 MAPK pathways by scopoletin reduce the inflammation caused by carrageenan in the mouse model of pleurisy

Nascimento

Arruda‐Silva

Luz

et al. 2016

Immunopharmacology and Immunotoxicology

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Natural products have long been used worldwide as therapeutic agents, but it is only recently, in response to the new challenges posed by global population aging, that interest in research into potentially therapeutic natural products has reemerged. In this context, coumarins, chemical compounds found in plants that have known anti-inflammatory activity, are promising candidates for the development of new drugs. In this study we test the effect of scopoletin, a coumarin found in several plant species, on carrageenan-induced inflammation in the mouse model of pleurisy. Initially, the effects of scopoletin on leukocyte migration and exudate concentrations were evaluated at three different doses (0.1, 1 and 5 mg/kg) and time (0.5-4 h before pleurisy). In the next step, we chose the lowest dose capable of inhibiting the inflammatory parameters (1 mg/kg), in order to analyze the myeloperoxidase and adenosine deaminase activities, the nitric oxide, tumor necrosis factor-α, and interleukin 1β levels in the fluid leakage, and the p65 subunit of NF-κB and p38 MAPK phosphorylation. Scopoletin at a dose of 1 mg/kg was able to significantly reduce cell migration and exudation to the pleural fluid (p < 0.01). Scopoletin at the same dose also decreased the myeloperoxidase and adenosine-deaminase activities and nitric oxide, tumor necrosis factor-α, and interleukin-1β levels (p < 0.01). In addition, it significantly reduced p65 and p38 phosphorylation in the mouse lungs (p < 0.01). Our results reinforce that scopoletin has important anti-inflammatory activity, and shows, that this effect can be attributed to the ability of this compound to inhibit the phosphorylation of NF-κB and p38 MAPK.

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“…Lung samples were taken and used to evaluate p65 (Nuclear factor kappa B subunit) and p38 [19][20][21].…”

Section: E P T E Dmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of the NF-κB and p38 MAPK pathways by scopoletin reduce the inflammation caused by carrageenan in the mouse model of pleurisy

Nascimento

Arruda‐Silva

Luz

et al. 2016

Immunopharmacology and Immunotoxicology

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“…As the total metabolism rate of mice is approximately seven times that of humans, 50 mg/Kg of DEC was adjusted according to the body weight [16]. The dose of indomethacin (5 mg/Kg) was based on a previous study [17]. …”

Section: Methodsmentioning

confidence: 99%

“…Total leukocytes were determined in a Neubauer chamber with the exudate diluted in Turk's solution (1 : 20) [17]. As the carrageenan-induced inflammatory response in the pleural space of mice has a biphasic profile, peaking at 4 and 48 h after pleurisy induction, the expression of inflammatory mediators was measured 4 h after the injection of carrageenan, based on previous studies [8].…”

Section: Methodsmentioning

confidence: 99%

Diethylcarbamazine Attenuates the Development of Carrageenan-Induced Lung Injury in Mice

Ribeiro

Barbosa

Fragoso

et al. 2014

Mediators of Inflammation

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Diethylcarbamazine (DEC) is an antifilarial drug with potent anti-inflammatory properties as a result of its interference with the metabolism of arachidonic acid. The aim of the present study was to evaluate the anti-inflammatory activity of DEC in a mouse model of acute inflammation (carrageenan-induced pleurisy). The injection of carrageenan into the pleural cavity induced the accumulation of fluid containing a large number of polymorphonuclear cells (PMNs) as well as infiltration of PMNs in lung tissues and increased production of nitrite and tumor necrosis factor-α and increased expression of interleukin-1β, cyclooxygenase (COX-2), and inducible nitric oxide synthase. Carrageenan also induced the expression of nuclear factor-κB. The oral administration of DEC (50 mg/Kg) three days prior to the carrageenan challenge led to a significant reduction in all inflammation markers. The present findings demonstrate that DEC is a potential drug for the treatment of acute lung inflammation.

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“…0.5 h before Cg induction. The inflammation was also analyzed after 24 h. The doses and pretreatment of the reference drugs were chosen based on previous studies in our laboratory and literature (Sedgwick and Lees 1986;Benincá et al 2007;Vigil et al 2008;Dalmarco et al 2009;de Oliveira et al 2014;Dos Reis et al 2014).…”

Section: Air Pouch Modelmentioning

confidence: 99%

Etanercept administration prevents the inflammatory response induced by carrageenan in the murine air pouch model

Mattei

Dalmarco

Fröde

2015

Naunyn-Schmiedeberg's Arch Pharmacol

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Rheumatoid arthritis (RA) is one of several inflammatory and autoimmune diseases that affect approximately 1% of world's population. The development of TNF inhibitors in the last decade represents a great advance in the treatment of mild and severe forms of RA. Etanercept is one of these drugs that is useful for RA treatment, but the mechanism of inhibition of the signaling pathway of inflammation was not completely elucidated. This study was conducted to evaluate the anti-inflammatory effect of etanercept in comparison to reference drugs (dexamethasone and indomethacin). Inflammation was induced by subcutaneal administration of carrageenan in the Swiss albino mice using the murine air pouch model. Exudation; leukocytes; myeloperoxidase (MPO); adenosine deaminase (ADA); nitric oxide metabolites (NOx); tumor necrosis factor (TNF); interferon gamma (IFN-γ); interleukins (IL) IL-6, IL-17, IL-10, IL-4, and IL-2; nuclear transcription factor kappa B (NF-κB) activation and apoptosis were evaluated 24 h after the induction of inflammation. Treatment with etanercept significantly inhibited exudate concentrations; leukocyte count; MPO and ADA activities; NOx, TNF, IFN-γ, and IL-17 levels; and NF-kappa B activation (p < 0.05). Etanercept induced apoptosis, reducing the number of viable neutrophils without increasing necrosis (p < 0.05). Our results suggest that the anti-inflammatory mechanism of action of etanercept may be via decrease of NF-κB activation. This effect promoted the reduction of pro-inflammatory cytokines and NOx and the induction of neutrophil apoptosis. The effect of etanercept upon neutrophils apoptosis may indicate the use of this drug therapy in the early stage of rheumatoid arthritis disease.

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Antioxidant effects of mycophenolate mofetil in a murine pleurisy model

Cited by 18 publications

References 27 publications

Inhibition of the NF-κB and p38 MAPK pathways by scopoletin reduce the inflammation caused by carrageenan in the mouse model of pleurisy

Inhibition of the NF-κB and p38 MAPK pathways by scopoletin reduce the inflammation caused by carrageenan in the mouse model of pleurisy

Diethylcarbamazine Attenuates the Development of Carrageenan-Induced Lung Injury in Mice

Etanercept administration prevents the inflammatory response induced by carrageenan in the murine air pouch model

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