Antioxidant effect of casein phosphopeptides compared with fruit beverages supplemented with skimmed milk against H2O2-induced oxidative stress in Caco-2 cells

Caseinophosphopeptides exert partial and site-specific cytoprotection against H2O2-induced oxidative stress in Caco-2 cells

“…Pretreatment of cells with CPPs before the oxidative stimuli produced an increase in GSH levels versus H 2 O 2 treatment (p < 0.05), without reaching the control baseline levels (see Table 4), which could be expected as preparation of the cell against potential oxidative injury. In contrast, Laparra et al (2008) have previously noted that pool A of CPPs did not increase GSH content under the same conditions. However, pretreatment for two hours with phosphopeptides of different kinds (hen egg yolk phosvitinPPPs) has also shown an increase in GSH values after H 2 O 2 -induced oxidative stress versus stress control Caco-2 cells (Katayama et al, 2006(Katayama et al, , 2007.…”

“…In this sense, Katayama, Xu, Fan, and Mine (2006) and Katayama, Ishikawa, Fan, and Mine (2007) have reported that oligophosphopeptides derived from hen egg yolk exert an antioxidant effect against H 2 O 2 , diminishing lipid peroxidation and interleukin-8 secretion, and causing the up-regulation of glutathione (GSH) induced biosynthesis, accompanied by an increase in glutathione reductase (GSH-Rd) and c-glutamylcysteine synthetase activity (c-GCS), as well as the induction of glutathione-S-transferase (GST) and catalase (CAT). To our knowledge, the antioxidant and cytomodulatory activity of CPPs has been previously reported in Caco-2 cells by Kibangou et al (2008); Laparra, Alegría, Barberá, and Farré (2008). The former authors studied the effect of a specific CPP (b-CN(1-25)) against lipid peroxidation, while the latter authors evaluated the effect of a pool of CPPs at 1.4 mg/ml (obtained by simulated gastrointestinal digestion) in comparison to fruit beverages with/without milk, against H 2 O 2 -induced oxidative stress, and reported a potential cytoprotective effect by preserving cell cycle distribution and GSH-Rd induction.…”

Free-radical-scavenging and anti-inflammatory effect of yak milk casein before and after enzymatic hydrolysis

“…Milk proteins are good sources of bioactive peptides which possess immunoregulatory, opioid, antioxidant and antihypertensive activities (Martin, Henry, & Bouhallab, 1999;Parrot, Degraeve, Curia, & Martial, 2003;Pihlanto, 2001). Casein phosphopeptides (CPPs), derived from casein, possess a potential cytoprotective effect against H 2 O 2 -induced oxidative stress by preserving cell biology and enzyme-mediated antioxidant systems (Laparra, Alegria, Barbera, & Farre, 2008). They have been shown to bind pro-oxidant metals, such as iron and to inhibit lipid oxidation as metal chelators (Kim, Jang, & Kim, 2007).…”

Changes in the color of white chocolate during storage: potential roles of lipid oxidation and non-enzymatic browning reactions

“…Among the biologically active peptide molecules, casein phosphopeptides (CPPs) have been proposed as potential dietary antioxidants on designing new functional products (Laparra et al 2008). They indicated that the CPPs contain phosphoseryl residues with the capacity to sequester potential prooxidant metals such as iron.…”