Caseinophosphopeptides exert partial and site-specific cytoprotection against H2O2-induced oxidative stress in Caco-2 cells

García-Nebot, María José; Cilla, Antonio; Alegrı́a, Amparo; Barberá, Reyes

doi:10.1016/j.foodchem.2011.05.129

Cited by 48 publications

(31 citation statements)

References 44 publications

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“…The present result showed that the applied hydrolysates especially MCH2-SF and MCH1-SF had stronger protection on the hepatocytes, based on significant decrease in cellular MDA. Similar conclusion had been given to caseinophosphopeptides and whey protein hydrolysates for the oxidative injury of Caco-2 cells and lung fibroblast MRC-5 cells (García-Nebot, Cilla, Alegría, & Barberá, 2011;Kong et al, 2012).…”

Section: Protective Effects Of the Casein Hydrolysates On Rat Hepatocsupporting

confidence: 61%

In vitrocytoprotection of modified casein hydrolysates by plastein reaction on rat hepatocyte cells

Zhao

Yang

2013

CyTA - Journal of Food

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Section: Protective Effects Of the Casein Hydrolysates On Rat Hepatocsupporting

confidence: 61%

In vitrocytoprotection of modified casein hydrolysates by plastein reaction on rat hepatocyte cells

Zhao

Yang

2013

CyTA - Journal of Food

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“…H 2 O 2 is able to induce oxidative stress injury, as reported by various studies (31,32); therefore, H 2 O 2 treatment was used in the present study to simulate oxidative stress. In our preliminary experiment, H 2 O 2 -induced oxidative stress was confirmed via the detection of higher ROS levels in cells treated by H 2 O 2 .…”

Section: Discussionmentioning

confidence: 89%

IGF-1 may predict the severity and outcome of patients with sepsis and be associated with microRNA-1 level changes

Zhang

Liu

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. IGF-1 functions as an anti-oxidative stress molecule and some critical patients with sepsis have a lower level of serum IGF-1. However, the association between IGF-1 and the severity or prognosis of sepsis remains unclear. This study aimed to elucidate the relationship between serum IGF-1 levels and the severity and prognosis of sepsis, and the possible mechanism was analyzed. Clinical characteristics of patients with sepsis were recorded and analyzed. Serum IGF-1 levels and micro (mi)RNA-1 levels were tested using radioimmunoassay and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, respectively. The A549 cell line and HKC cell line were cultured in vitro and exposed to H 2 O 2 with or without IGF-1 treatment. Cell death was detected by analyzing cell death markers via ELISA kits, and miRNA-1 levels were detected after H 2 O 2 exposure using RT-qPCR analysis. miRNA-1 in cells was upregulated by transfection and IGF-1 mRNA was detected to determine its relationship with miRNA-1. Once again, cell ELISA kits were used to analyze cell death markers after transfection. Serum IGF-1 levels were reduced in patients with sepsis, whereas miRNA-1 levels were higher (P<0.05 vs. healthy control). Patients in the septic shock subgroup or dead patients had the lowest IGF-1 levels and the highest miRNA-1 levels (P<0.05 vs. sepsis and severe sepsis). IGF-1 levels were inversely proportional to the miRNA-1 level. In vitro, IGF-1 reduced the cell death caused by H 2 O 2 . miRNA-1 transfection effectively increased the sensitivity of cells to H 2 O 2 damage by reducing the expression of IGF-1, which was able to prevent cells from injury caused by H 2 O 2 . The transfection of negative control miRNA did not influence the level of IGF-1 miRNA and the sensitivity to H 2 O 2 damage. In conclusion, low IGF-1 levels in patients with sepsis may predict increased severity of the condition and poor prognosis. The possible mechanism is that the excessive miRNA-1 levels reduce IGF-1 levels, resulting in insufficient anti-oxidative action by IGF-1 which increases the injury caused by oxidative stress in patients with sepsis.

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“…It has been reported that casein phosphopeptides and glycine exhibit apparent cytoprotective effect against H 2 O 2 ‐induced oxidative stress by using Caco‐2 cells as the human intestine model. Moreover, it has been demonstrated that the principal cytoprotective effect of the active component on Caco‐2 cells is exhibited through modulating the cellular damage generated by the oxidative challenge, or enhancing the epithelial cell defense system and maintaining the potential of intracellular antioxidant capability (Howard and others ; García‐Nebot and others ).…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, there is an increasing interest in novel tyrosinase inhibitors from natural sources (Chang ), which have the potential to improve food quality by controlling discoloration of fresh‐cut fruits and vegetables, seafood melanosis, and the color of various commercial foods (Yu and others ). In addition, it has been reported that remarkable cytoprotection against H 2 O 2 ‐induced oxidative stress in Caco‐2 cells is exhibited by certain botanical extracts (García‐Nebot and others ), which are likely to be used as functional additives to maintain and protect the human intestine. Hence, many studies have focused on the application of bioactive extracts from food materials as novel food additives, which may be perceived as good alternatives to synthetic additives in the food industry (Amakura and others ; Wu and others ).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant, Antibrowning, and Cytoprotective Activities of Ligustrum robustum (Rxob.) Blume Extract

Zeng

2013

Journal of Food Science

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The antioxidant, antibrowning, and cytoprotective activities of Ligustrum robustum (Rxob.) Blume extract (LRE) were investigated and the main antioxidant component was isolated and identified. With its high content of phenols and flavonoids, the LRE showed remarkable antioxidant capacity to scavenge free radicals in vitro and to inhibit oil oxidation in a peanut oil system. Moreover, LRE was observed to inhibit tyrosinase action and browning of fresh-cut apple slices effectively. Furthermore, the cytoprotective activity of LRE was evaluated in a human intestine model using Caco-2 cell lines. According to the activity-guided isolation and identification, by using column chromatography, high-performance liquid chromatography, time-of-flight mass spectrometry, and nuclear magnetic resonance analyses, ursolic acid was characterized as the main antioxidant component of LRE; it showed the strongest free radical-scavenging activity. The results suggested that L. robustum (Rxob.) Blume could be a new resource for preparing functional food and nutraceutical products for use in food and pharmacology industries.

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Caseinophosphopeptides exert partial and site-specific cytoprotection against H2O2-induced oxidative stress in Caco-2 cells

Cited by 48 publications

References 44 publications

In vitrocytoprotection of modified casein hydrolysates by plastein reaction on rat hepatocyte cells

In vitrocytoprotection of modified casein hydrolysates by plastein reaction on rat hepatocyte cells

IGF-1 may predict the severity and outcome of patients with sepsis and be associated with microRNA-1 level changes

Antioxidant, Antibrowning, and Cytoprotective Activities of Ligustrum robustum (Rxob.) Blume Extract

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