Antioxidant Content, Antioxidant Activity, and Antibacterial Activity of Five Plants from the Commelinaceae Family

Abstract

Commelinaceae is a family of herbaceous flowering plants with many species used in ethnobotany, particularly in South America. However, thus far reports of their bioactivity are few and far between. The primary aim of this study was to quantify the antioxidant and antibacterial activity of five Commelinaceae methanolic leaf extracts. The antioxidant content was evaluated by the total phenolic content (TPC), total tannin content (TTC), and total flavonoid content (TFC) assays. The antioxidant activities measured were DPPH free radical scavenging (FRS), ferric reducing power (FRP), and ferrous ion chelating (FIC); of the five plants, the methanolic leaf extract of Tradescantia zebrina showed the highest antioxidant content and activity, and exhibited antibacterial activity against six species of Gram-positive and two species of Gram-negative bacteria in a range of 5–10 mg/mL based on the broth microdilution method.

Antioxidant, Antifungal Activities of Ethnobotanical Ficus hirta Vahl. and Analysis of Main Constituents by HPLC-MS

“…Three phenolics, namely, luteolin, apigenin, and umbelliferone, were putatively identified by comparison of extracted positive or negative ions with literature data, for which those were the main constituents previously reported [2,3]. Five flavonoid constituents (13)(14)(15)(16)(17) were all detected in EAE, AE, and ME of HFF. Furthermore, pinocembrin-7-O-β-D-glucoside (13), the main antifungal flavonoid, was also detected in CE of HFF.…”

“…The total flavonoid contents (TFC) in various HFF extracts were analyzed using the aluminum chloride method [17] with few modifications. Briefly, 1.0 mL of each extract solution was mixed with 2.0 mL of 0.1 mM aluminum chloride, 1.0 mL of 1 M sodium acetate (pH 5.2), and 6.0 mL of methanol.…”

“…The FRAP assay of HFF extracts was applied with some modifications to reduce ferric ions (Fe 3+ ) [17]. Briefly, the mixture containing 1.0 mL of 50 mM phosphate buffer (pH 6.6) and 1.0 mL of 1% (w/v) potassium ferricyanide was added to 0.5 mL of each extract (concentrations ranged from 2 to 10 mg/mL).…”

In vitro biological assessment of Homalium zeylanicum and isolation of lucidenic acid A triterpenoid

“…The scavenging activity of HAHZ on the stable free radical DPPH was assayed using the modified protocol in which the bleaching rate of DPPH was monitored at a characteristic wavelength in the presence of the sample [12] . Stock solution of 1 mg/mL was prepared in methanol.…”

Traditional Herbal Medicine in Mesoamerica: Toward Its Evidence Base for Improving Universal Health Coverage

“…Antibacterial: methanolic leaf extracts showed MIC values of 5-10 mg/ml against 12 Gram-positive and -negative strains (Tan et al, 2014). Antibacterial (incl.…”