In vitro biological assessment of Homalium zeylanicum and isolation of lucidenic acid A triterpenoid

Sahoo, Atish Kumar; Dash, Umesh Chandra; Kanhar, Satish; Mahapatra, A. K.

doi:10.1016/j.toxrep.2017.04.004

Cited by 14 publications

(9 citation statements)

References 33 publications

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“…So far, available studies on Homalium spp. have demonstrated relevant biological activities frequently related to their ethnomedicinal uses, such as antibacterial [2], antidiabetic [3], antinociceptive [4] and anti-inflammatory [3,4].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Proinflammatory Enzymes and Attenuation of IL-6 in LPS-Challenged RAW 264.7 Macrophages Substantiates the Ethnomedicinal Use of the Herbal Drug Homalium bhamoense Cubitt & W.W.Sm

Suksungworn

Andrade

Oliveira

et al. 2020

IJMS

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Commonly used to treat skin injuries in Asia, several Homalium spp. have been found to promote skin regeneration and wound healing. While ethnobotanical surveys report the use of H. bhamoense trunk bark as a wound salve, there are no studies covering bioactive properties. As impaired cutaneous healing is characterized by excessive inflammation, a series of inflammatory mediators involved in wound healing were targeted with a methanol extract obtained from H. bhamoense trunk bark. Results showed concentration-dependent inhibition of hyaluronidase and 5-lipoxygenase upon exposure to the extract, with IC50 values of 396.9 ± 25.7 and 29.0 ± 2.3 µg mL−1, respectively. H. bhamoense trunk bark extract also exerted anti-inflammatory activity by significantly suppressing the overproduction of interleukin 6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages at concentrations ranging from 125 to 1000 µg mL−1, while leading to a biphasic effect on nitric oxide (NO) and tumor necrosis factor alpha (TNF-α) levels. The phenolic profile was elucidated by HPLC-DAD, being characterized by the occurrence of ellagic acid as the main constituent, in addition to a series of methylated derivatives, which might underlie the observed anti-inflammatory effects. Our findings provide in vitro data on anti-inflammatory ability of H. bhamoense trunk bark, disclosing also potential cutaneous toxicity as assessed in HaCaT keratinocytes.

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Section: Introductionmentioning

confidence: 99%

Inhibition of Proinflammatory Enzymes and Attenuation of IL-6 in LPS-Challenged RAW 264.7 Macrophages Substantiates the Ethnomedicinal Use of the Herbal Drug Homalium bhamoense Cubitt & W.W.Sm

Suksungworn

Andrade

Oliveira

et al. 2020

IJMS

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“…the, the reaction mixture was allowed to cool to room temp and absorbance was recorded at 532 nm by using microplate Reader (Bio-Tek Synergy H1MF, USA). 13 Ascorbic acid was used as reference drug.…”

Section: % Inhibition = (A Control -A Sample )/A Control × 100mentioning

confidence: 99%

“…Nitric oxide radical scavenging activity Hydroalcohol extracts of H. zeylanicum (HZB and HZL, 50-200 µg/ml) was mixed with sodium nitroprusside (10 mM) and incubated at 25 °C for 1.5 h. Then the reaction mixture was added to Griess reagent (1 ml) and absorbance was recorded at 546 nm by using microplate Reader (Bio-Tek Synergy H1MF, USA). 13 Quercetin was used as reference drug.…”

Section: % Inhibition = (A Control -A Sample )/A Control × 100mentioning

confidence: 99%

“…After five min incubation at room temp, absorbance of the reaction mixture was recorded at 515 nm by using microplate Reader (Bio-Tek Synergy H1MF, USA). 13 Ascorbic acid was used as reference drug.…”

mentioning

confidence: 99%

“…Then, absorbance was read at 560 nm by using microplate Reader (Bio-Tek Synergy H1MF, USA). 13 Quercetin was used as reference drug.…”

mentioning

confidence: 99%

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Antioxidant and Hepatoprotective Activities of Hydroalcohol Extract of Homalium zeylanicum on Carbon tetrachloride Induced Liver Damage in Wistar Rats

Kanhar¹,

Sahoo²,

Mohapatra³

2021

JYP

Self Cite

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Liver is a pivotal organ in human for metabolism and detoxification of xenobiotics, environmental pollutants, and chemotherapeutic agents and regulates cellular homeostasis. 1 It is more susceptible to get damaged by various hepatotoxicants. In recent years, liver diseases greatly impact global health care system. The plant based drugs like Silybum marianum, Phyllanthus emblica, Picrorhiza kurroa etc. are widely and successfully used in the treatment of liver disorders. But still, there is need of less toxic and high efficacy plant based drugs. [2][3][4] Homalium zeylanicum Benth. is predominantly found in evergreen forest and native to Eastern ghats of Andhra Pradesh of India. Previously, the plant was placed in the family Flacourtiaceae but in recent Angiosperm Phylogeny Group (APG)-IV system of classification, it is placed in Salicaceae. Traditional tribes of Rayalaseema region of Andhra Pradesh use leaves and bark of H. zeylanicum in the treatment of malaria, diabetes, rheumatism, wound healing, and inflammation. [5][6][7] In our previous studies, we have reported the presence of phenolics, flavonoids, steroids and terpenes in the species. 8 Experimental evidences reflect antioxidant, anti-inflammatory, antidiabetic, hepatoprotective, antidyslipidemic, and anthelmintic properties. 9-14 On basis of our earlier studies on hepatoprotective activities of ethyl acetate extract of H. zeylanicum, we aimed to establish antioxidant and hepatoprotective effect of hydroalcohol extract of H. zeylanicum on carbon tetrachloride induced liver cirrhosis in wistar rats. MATERIALS AND METHODS Chemicals, reagents and assay kitsAll chemicals, reagents and solvents were of analytical grade and purchased from

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A review of alpha-glucosidase inhibitors from plants as potential candidates for the treatment of type-2 diabetes

et al. 2021

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Diabetes mellitus is a multifactorial global health disorder that is rising at an alarming rate. Cardiovascular diseases, kidney damage and neuropathy are the main cause of high mortality rates among individuals with diabetes. One effective therapeutic approach for controlling hyperglycemia associated with type-2 diabetes is to target alpha-amylase and alpha-glucosidase, enzymes that catalyzes starch hydrolysis in the intestine. At present, approved inhibitors for these enzymes are restricted to acarbose, miglitol and voglibose. Although these inhibitors retard glucose absorption, undesirable gastrointestinal side effects impede their application. Therefore, research efforts continue to seek novel inhibitors with improved efficacy and minimal side effects. Natural products of plant origin have been a valuable source of therapeutic agents with lesser toxicity and side effects. The anti-diabetic potential through alpha-glucosidase inhibition of plant-derived molecules are summarized in this review. Eight molecules (Taxumariene F, Akebonoic acid, Morusin, Rhaponticin, Procyanidin A2, Alaternin, Mulberrofuran K and Psoralidin) were selected as promising drug candidates and their pharmacokinetic properties and toxicity were discussed where available. Supplementary Information The online version contains supplementary material available at 10.1007/s11101-021-09773-1.

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In vitro biological assessment of Homalium zeylanicum and isolation of lucidenic acid A triterpenoid

Abstract: Graphical abstract

Cited by 14 publications

References 33 publications

Inhibition of Proinflammatory Enzymes and Attenuation of IL-6 in LPS-Challenged RAW 264.7 Macrophages Substantiates the Ethnomedicinal Use of the Herbal Drug Homalium bhamoense Cubitt & W.W.Sm

Inhibition of Proinflammatory Enzymes and Attenuation of IL-6 in LPS-Challenged RAW 264.7 Macrophages Substantiates the Ethnomedicinal Use of the Herbal Drug Homalium bhamoense Cubitt & W.W.Sm

Antioxidant and Hepatoprotective Activities of Hydroalcohol Extract of Homalium zeylanicum on Carbon tetrachloride Induced Liver Damage in Wistar Rats

A review of alpha-glucosidase inhibitors from plants as potential candidates for the treatment of type-2 diabetes

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