Antioxidant capacity responsible for a hypocholesterolemia is independent of dietary cholesterol in adult rats fed rice protein

In vitro antioxidant activity of rice protein affected by alkaline degree and gastrointestinal protease digestion

“…and in the methionine sulfoxide reductase antioxidant system . Furthermore, recent in vivo progress indicated that higher content of SAAs in RP might represent a possible mechanism for improving oxidative stress through depressing lipid peroxidation and stimulating GSH synthesis . Significantly, the present findings provided supportive in vitro evidence for the view that RP could attenuate the oxidative damage to lipids, which is the main cause of hypercholesterolaemia, strongly highlighting the key role of SAAs in the antioxidant status.…”

“…In this study, the reducing power assay was used to evaluate the antioxidant ability of RP through donating electron to free radicals and reacting with free radicals, hence terminating the free radical chain reaction . Consequently, the evidence strongly supported our in vivo findings that RP could stimulate the reduction of oxidised glutathione (GSSG) to GSH, resulting in an antioxidative status through the endogenous GSH pool slowly shifting towards the oxidised state …”

“…RP could significantly suppress ROS production and attenuate the oxidation of lipids and proteins in in vivo system, suggesting that the removal of free radicals is achieved through enzymatic and non‐enzymatic reactions . The biological utilisation of a protein is primarily dependent on its digestibility by gastric, pancreatic, and intestinal peptidases, providing the insight that the digestion may play a major role in the modulation of physiological function of dietary protein .…”

“…As a major dietary source of protein for humans, rice protein (RP) has attracted considerable attention for its physiological functions . Recently, a series of in vivo studies demonstrated that RP could significantly reduce hepatic contents of malondialdehyde (MDA) and protein carbonyl (PCO), which were two major biomarkers of oxidative stress, suggesting that the status of depressing lipid peroxidation and protein oxidation was consequent upon attenuating the susceptibility to reactive oxygen species (ROS)‐mediated damage by RP‐feeding . These findings provide evidence that RP can produce an antioxidative response that prevents the occurrence of hyperlipidaemia through regulating glutathione (GSH) metabolism and attenuating oxidative damage to lipids, proteins and ROS .…”

“…Recently, a series of in vivo studies demonstrated that RP could significantly reduce hepatic contents of malondialdehyde (MDA) and protein carbonyl (PCO), which were two major biomarkers of oxidative stress, suggesting that the status of depressing lipid peroxidation and protein oxidation was consequent upon attenuating the susceptibility to reactive oxygen species (ROS)‐mediated damage by RP‐feeding . These findings provide evidence that RP can produce an antioxidative response that prevents the occurrence of hyperlipidaemia through regulating glutathione (GSH) metabolism and attenuating oxidative damage to lipids, proteins and ROS . Moreover, a molecular antioxidant mechanism exerted by RP has been elicited in adult rats under cholesterol‐free and cholesterol‐enriched dietary conditions, indicating that RP can stimulate the antioxidant/detoxification gene expressions of glutamate cysteine ligase catalytic subunit (GCLC), glutamate cysteine ligase modulatory subunit (GCLM), glutathione S ‐transferase (GST), haem oxygenase 1 (HO‐1) and NAD(P)H:quinone oxidoreductase 1 (NQO1) through the activation of nuclear factor erythroid 2 (NF‐E2)‐related factor 2 (Nrf2), an important transcription factor involving in the endogenous antioxidant defence against oxidative damage .…”