Antioxidant capacity of four polyphenol-rich Amazonian plant extracts: A correlation study using chemical and biological in vitro assays

Souza, Jesus Nazareno Silva de; Silva, Evaldo Martins da; Loir, Adeline; Rees, Jean-François; Rogez, Hervé; Larondelle, Yvan

doi:10.1016/j.foodchem.2007.05.011

Cited by 90 publications

(60 citation statements)

References 37 publications

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“…PCs were quantified by HPLC using a previously described methodology (Souza et al, 2008). The system and operating conditions were as follows: a Shimadzu series LC-10Avp (Tokyo, Japan) HPLC apparatus paired with an SPD-M20A diode array detector, an automatic SIL-10AF injector, a column oven at 30 °C, a precolumn Gemini analyzer with a 3.0 × 4.0 mm internal diameter and a Gemini C 18 column of 250 × 4.6 mm internal diameter with a particle size of 5 μm (Phenomenex, Torrance, CA) and the Class VP chromatography data station software (Shimadzu).…”

Section: Quantification Of the Phenolic Compoundsmentioning

confidence: 99%

Adsorption capacity of phenolic compounds onto cellulose and xylan

Costa¹,

Rogez²,

Pena³

2015

Food Sci. Technol (Campinas)

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The interaction between three phenolic compounds (catechin, caffeic acid and ferulic acid) onto two dietary fibres (cellulose and xylan) has been evaluated to inquire possible interferences on the biodisponibility of phenolic compounds. The adsorption kinetics were performed using solutions containing 100 mg/L of phenolic compounds during a contact time ranging between 10 and 120 minutes at pH 2.0, 4.5, and 7.0. After the kinetics, isotherms were obtained using phenolic compounds concentration ranging between 10 and 80 mg/L during 60 minutes, at pH 2.0 and 7.0 and temperature of 36 °C. Results indicate that adsorbed quantities mainly changed in function of pH, however the maximum adsorption was only of 0.978 mg of caffeic acid/g of xylan at pH 2 and after 60 min. Redlich-Peterson model were able to predict the adsorption isotherms of all phenolic compounds onto cellulose, except for caffeic acid at pH 7.0. The low adsorption capacities observed suggest that both dietary fibres are unable to compromise the biodisponibility of phenolic compounds, especially in the small intestine, where they are partially absorbed.

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Section: Quantification Of the Phenolic Compoundsmentioning

confidence: 99%

Adsorption capacity of phenolic compounds onto cellulose and xylan

Costa¹,

Rogez²,

Pena³

2015

Food Sci. Technol (Campinas)

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“…Diversas espécies são utilizadas popularmente para o tratamento das mais variadas afecções, necessitando de estudos aprofundados para comprovar suas indicações. a exploração racional destas espécies poderia contribuir para o desenvolvimento sustentável da região (Souza et al 2008). entre estas espécies pode-se citar o murucizeiro, Byrsonima crassifolia (l) H.B.…”

Section: Antioxidant Capacity and Pharmacologic Screening Of Crude Exunclassified

“…Silva et al (2007a;2007b) apontaram elevada capacidade antioxidante do seu extrato hidroalcoólico. Souza et al (2008) apontaram alta capacidade antioxidante empregando diversos métodos químicos e bioquímicos, avaliando a proteção de lipoproteínas e de eritrócitos contra a oxidação induzida de diferentes formas. estudos fitoquímicos identificaram parte dos compostos fenólicos presentes nos extratos de folhas de I. edulis (Souza et al 2007;Dias et al 2010).…”

Section: Antioxidant Capacity and Pharmacologic Screening Of Crude Exunclassified

“…neste estudo, os autores identificaram o acido gálico, a catequina, a epicatequina, a miricetina-3-ramnopiranosídeo, a quercetina-3-glucopiranosídeo e a quercetina-3-ramnopiranosídeo. Souza et al (2008) utilizaram solução hidrometanólica (80:20 v/v) durante 3 h a temperatura ambiente. Dias et al (2010) utilizaram uma mistura de solventes (acetona:água:ácido acético; 70:28:2; v:v:v), durante 2 h sob agitação constante, à temperatura ambiente, um total de três ciclos de extração.…”

Section: Otimizaram a Extraçãounclassified

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Capacidade antioxidante e triagem farmacológica de extratos brutos de folhas de Byrsonima crassifolia e de Inga edulis

et al. 2012

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ResumoCom o objetivo de avaliar o efeito de duas espécies amazônicas em doenças relacionadas aos processos de oxidação, determinouse a capacidade antioxidante (método Oxygen Radical Absorbance Capacity), o teor de polifenóis totais (método Folin-Ciocalteu -Pt), bem como os efeitos farmacológicos in vitro (efeito antiproliferativo) e in vivo (antinociceptivo, antiinflamatório, antiulcerogênico) dos extratos hidroalcoólicos (65:35; v/v; etanol:água) ) de sua administração oral. nos modelos de inflamação houve somente redução do edema para ie na concentração de 500 mg kg -1 . os extratos das duas espécies reduziram as lesões ulcerativas produzidas por etanol em até 84% (P < 0,05), sugerindo uma possível ligação com a atividade antioxidante observada e indicando a necessidade de estudos para a elucidação do mecanismo de ação envolvido. PalavRas-Chaves: amazônia; oxygen radical absorbance capacity; Polifenóis; Propriedades farmacológicas. Antioxidant capacity and pharmacologic screening of crude extracts ofByrsonima crassifolia and Inga edulis leaves abstRaCt in order to evaluate the effect of two amazonian species on chronic diseases linked with the oxidative processes, we performed antioxidant capacity analyses (oxygen Radical absorbance Capacity -oRaC and Folin-Ciocalteu -Pt assays) and pharmacological effects in vitro (antiproliferative effect) and in vivo (antinociceptive, antiinflammatory, antiulcerogenic effects) for ethanolic extracts (65:35; v/v; ethanol:water) from Byrsonima crassifolia (BC) and Inga edulis (ie) leaves. Both BC and ie extracts showed high oRaC values (1,422 and 694 mmol of trolox equivalent/g of dry leaf, respectively) and high Pt contents (35.93 and 24.50 mg gallic acid equivalent g -1 dry leaf, respectively). The oRaC values had no correlation with Pt, suggesting the presence of other chemical groups in the antioxidant activity value. The two extracts did not present significant antiproliferative activity on nine lines of human tumor cells, and cytotoxic effect was detected only at the highest concentration. The antinociceptive effect was investigated using the hot plate test, and ie extract presented a longer latency (P < 0.05) 30 and 60 min after oral administration. The antiinflammatory activity was only observed at the highest concentration, suggesting that the antinociceptive effect observed was not due to the antiinflammatory effect. The extracts of both species reduced the ulcerative lesions produced by ethanol up to 84% (P < 0.05), suggesting a relation with the antioxidant capacity. more studies are necessary to elucidate the mechanisms of action involved on antiulcerative effects.

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“…Furthermore, the P. edulis ethanolic extract phenolic content was higher than the concentrations that were observed in different studies with other herbal extracts that have potential anti-inflammatory and hypoglycaemic properties (Caia et al, 2004;Souza et al, 2008).…”

Section: Resultsmentioning

confidence: 58%

Ethanolic extract of Passiflora edulis Sims leaves inhibits protein glycation and restores the oxidative burst in diabetic rat macrophages after Candida albicans exposure

Martins

Salles

Brigagão

et al. 2015

Braz. J. Pharm. Sci.

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This study was conducted to evaluate the effects of the ethanolic extract of Passiflora edulis leaves on blood glucose, protein glycation, NADPH oxidase activity and macrophage phagocytic capacity after Candida albicans exposure in diabetic rats. The Passiflora edulis Sims leaves were dried to 40°C, powdered, extracted by maceration in 70% ethanol, evaporated under reduced pressure and lyophilised. The biochemical tests performed were total phenolic content (TP) as determined by the Folin-Ciocalteu assay, trapping potential DPPH assay and total iron-reducing potential. Diabetes was induced by alloxan injection. Protein glycation was determined by AGE and fructosamine serum concentrations. Extracttreated diabetic animals demonstrated lower fructosamine concentrations compared with the diabetic group. Our results suggest that ethanolic Passiflora edulis Sims leaf extraction may have beneficial effects on diabetes and may improve glycaemic control in diabetic rats.Uniterms: Passiflora edulis Sims/pharmacognosy. Passiflora edulis Sims/ethanolic extract/effects. Blood glucose. Protein glycation. Reactive oxygen species. Diabetes/treatment/experimental study. Medicinal plants.O objetivo deste estudo foi avaliar os efeitos do extrato etanólico de folhas de Passiflora edulis sobre os níveis de glicose sanguínea, glicação protéica, produção de espécies reativas de oxigênio (ERO) e capacidade fagocítica de macrófagos de ratos diabéticos. As folhas de Passiflora edulis Sims foram secas a 40 °C, trituradas e o extrato preparado por maceração em solução hidroetanólica 70% (v/v) etanol foi evaporado sob pressão reduzida e liofilizado. Os testes químicos realizados demonstraram que além da presença de compostos fenólicos, determinada pelo método de Folin-Ciocalteu, o extrato apresentou potencial sequestrante de radicais DPPH e redutor de ferro. Nos animais diabéticos foi observado aumento na glicação protéica, avaliada pela concentração de frutosaminas e de produtos de glicação avançada (AGE), e redução na produção de ERO por macrófagos frente à Candida albicans, quando comparados ao grupo controle. O tratamento dos animais diabéticos com o extrato reduziu as concentrações de frutosaminas e manteve a produção de ERO em níveis semelhantes aos observados no grupo controle. Nossos resultados sugerem que o extrato etanólico de folhas de Passiflora edulis Sims pode apresentar efeitos benéficos sobre o diabetes e melhorar o controle glicêmico em ratos diabéticos.Unitermos: Passiflora edulis Sims/farmacognosia. Passiflora edulis Sims/extrato etanólico/efeitos. Glicose sanguínea. Glicação protéica. Espécies reativas de oxigênio. Diabetes/tratamento/estudo experimental. Plantas medicinais.

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Antioxidant capacity of four polyphenol-rich Amazonian plant extracts: A correlation study using chemical and biological in vitro assays

Cited by 90 publications

References 37 publications

Adsorption capacity of phenolic compounds onto cellulose and xylan

Adsorption capacity of phenolic compounds onto cellulose and xylan

Capacidade antioxidante e triagem farmacológica de extratos brutos de folhas de Byrsonima crassifolia e de Inga edulis

Ethanolic extract of Passiflora edulis Sims leaves inhibits protein glycation and restores the oxidative burst in diabetic rat macrophages after Candida albicans exposure

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