Fruits of Euterpe oleracea (FEO) are currently known as elements that present a very high antioxidant activity (AAO), as measured by the Oxygen Radical Absorbance Capacity. They are particularly rich in total phenolics (TP) and total anthocyanins (TA). Response Surface Methodology was used to optimise the solvent extraction of phenolic antioxidants from FEO, using a second-order polynomial equation to describe the experimental data for TP, TA, and AAO. In order to determine the best solid-to-liquid ratio and time of extraction, some preliminary studies were conducted. A rotatable central composite design with three variables (ethanol proportion, hydrochloric acid concentration and temperature) was then used. The results showed a good fit to the proposed model (R(2)>0.89). TP and TA, as well as TA and AAO, showed significant correlations (P<0.05). The optimised conditions that maximized the yields of phenolic compounds (TP and TA) and AAO from FEO were: ethanol proportion between 70% and 80%, hydrochloric acid concentration between 0.065 and 0.074 mol/L and a temperature of 58 degrees C.
Um extrato metanol-água das folhas de Inga edulis foi fracionado para identificar os compostos polifenólicos. Os compostos identificados foram o acido gálico, a catequina, a epicatequina, a miricetina-3-ramnopiranosídeo, a quercetina-3-glucopiranosídeo e a quercetina-3-ramnopiranosídeo. A capacidade antioxidante do extrato e dos polifenóis puros foi medida pelo teste ORAC e comparada com o teor em fenólicos totais (TP). O extrato bruto seco apresentou valores de ORAC (11.16 mmol TE per g) e TP (496.5 mg GAE per g) muito altos. Os compostos identificados foram responsáveis, respectivamente, por 9.53 % e 12.10 % dos valores ORAC e de TP do extrato de folhas de Inga edulis.A methanol-water extract of Inga edulis leaves was fractionated to identify the polyphenolic compounds. The identified compounds were gallic acid, catechin, epicatechin, myricetin-3-rhamnopyranoside, quercetin-3-glucopyranoside and quercetin-3-rhamnopyranoside. The antioxidant activity of the extract and of pure polyphenols was measured by the ORAC assay and compared with the Total Phenolic (TP) content. The dry crude extract presented very high values for ORAC (11.16 mmol TE per g) and TP (496.5 mg GAE per g). The identified compounds were responsible for 9.53 % and 12.10 % of the ORAC value and TP content of the Inga edulis leaf extract, respectively.
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