Antioxidant, Biomolecule Oxidation Protective Activities of Nardostachys jatamansi DC and Its Phytochemical Analysis by RP-HPLC and GC-MS

Razack, Sakina; Kandikattu, Hemanth Kumar; Nallamuthu, Ilaiyaraja; Naika, Mahadeva; Khanum, Farhath

doi:10.3390/antiox4010185

Cited by 57 publications

(23 citation statements)

References 57 publications

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“…Therefore, the single band appeared at sites 1 and 3, which indicated the plasmid was not affected by the oxidizing agent due to the presence of compound (1,5-dimethyl-2-phenyl-4-((2,3,4-trihydroxy phenyl) diazenyl)-1H-pyrazol-3(2H)-one) working as antioxidants. These results are consistent with many studies such as those conducted by Razack et al [13] and by Lahneche et al [14].…”

Section: Antioxidantssupporting

confidence: 93%

Spectrophotometric Determination of Cobalt(II) and Lead(II) Using (1,5-Dimethyl-2-Phenyl-4-((2,3,4-Trihydroxy Phenyl) Diazenyl)-1H-Pyrazol-3(2H)-One) as Organic Reagent: Using It as Antimicrobial and Antioxidants

Essa¹,

Hoidy²

2020

Nano BioMed ENG

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The azo organic reagent (1,5-dimethyl-2-phenyl-4-((2,3,4-trihydroxy phenyl) diazenyl)-1H-pyrazol-3(2H)-one) (DPTPD) was prepared and used for the spectrophotometric determination of cobalt(II) and Lead(II), by the selective and surfactant-sensitized method based on the ternary complexes formation of Co(II) and Pb(II). The reagent had absorption maximum at 381 nm, and reacted with Co 2+ to form a purple reddish complex with λ max = 430 nm at pH = 7.5, while it formed a red complex with Pb 2+ of λ max = 417 nm at pH= 6. Beer's law for the determination over the range of 1-25 ppm and 1-33 ppm for Co(II) and Pb(II), respectively. The molar absorptivity (Є) and Sandell's sensitivity values of Co(II) and Pb(II) complexes were found to be 1.02×10 4 , 3.3×10 4 mol-1 cm-1 , and 0.0725, 0.0269 μg cm-2 at 430, 417 nm, respectively. The stability constant was found to be 1.1×10 8 L mol-1 and 2.3×10 8 L mol-1 for Co(II) and Pb(II), respectively. Detection limit relative standard deviation, relative error and recovery were predestined for 15 ppm standard solution of Co(II) and Pb(II) complexes, respectively. The important interferences with most ions like Cr +3 , Mn 2+ , Fe 3+ , Zn 2+ , Hg 2+ , Mo +2 , Pt 2+ and Cd +2 were studied using the appropriate masking agents. The method was applied for the determination of Co(II) in filling sample; the result obtained was incompatible with that by the flame atomic absorption spectrometry method. The organic reagent (DPTPD) was diagnosed as an antibacterial and an antioxidant.

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Section: Antioxidantssupporting

confidence: 93%

Spectrophotometric Determination of Cobalt(II) and Lead(II) Using (1,5-Dimethyl-2-Phenyl-4-((2,3,4-Trihydroxy Phenyl) Diazenyl)-1H-Pyrazol-3(2H)-One) as Organic Reagent: Using It as Antimicrobial and Antioxidants

Essa¹,

Hoidy²

2020

Nano BioMed ENG

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“…The phenolics have the free radical scavenging, chelation of redox-active metal ions, modulation of gene expression and interaction with the cell signaling pathways. 60 , 61 , 62 , 63 The plasmid DNA pUC19 which consists of 3 forms single-stranded relaxed nicked DNA (R Form) and double-stranded nicked and linear DNA (L-Form). Plasmid DNA damage caused by hydroxyl (•OH) radicals generates Fenton reaction which makes DNA strands to yield its open circular or relaxed forms.…”

Section: Discussionmentioning

confidence: 99%

Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulis

Rachitha

Krupashree

Jayashree

et al. 2018

Journal of Traditional and Complementary Medicine

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Herbal medicines are known to mitigate radical induced cell damage. Hence identification and scientific validation of herbal medicines contribute to better use in Ayurvedic/Unani research. In the present study, we investigated antioxidant and anti-apoptotic properties of Convolvulus pluricaulis (C. pluricaulis). C. pluricaulis exhibited antioxidant potential evident by free radical scavenging activities. C. pluricaulis pretreatment inhibited H2O2 induced macromolecule damage such as plasmid DNA damage and AAPH induced oxidation of bovine serum albumin and lipid peroxidation of rat hepatic tissues. Further to identify the neuroprotective properties of C. pluricaulis, SHSY5Y cells were treated with H2O2 with or without pretreatment of C. pluricaulis. The C. pluricaulis pretreatment at 50 μg/ml dose exhibited 50% cell survival against 100 μM H2O2 challenge for 24 h and it also decreased the lactate dehydrogenase leakage. Further C. pluricaulis pretreatment restored and regulated the antioxidant and apoptosis markers such as SOD, CAT, p53, and caspase-3 and inhibited, reactive oxygen species generation and depolarization of the mitochondrial membrane. C. pluricaulis possess a high content of flavonoids and polyphenols and GC-MS and FTIR analysis showed a wide variety of compounds which may contribute to the observed effects.

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“…In recent years, Fourier-transform infrared (FTIR) and gas chromatography-mass spectrometry (GC-MS) has commonly been employed for detection of functional groups and identification of various bioactive therapeutic compounds that are present in medicinal plants 13,14 . GC-MS is one of the best, fast and accurate techniques to detect various compounds, including alcohols, alkaloids, nitro compounds, long chain hydrocarbons, organic acids, steroids, esters and amino acids 15 , and requires a small volume of plant extracts. Hence, in the present study, GC-MS technique was adopted for detection and identification of phytochemical compounds present in the medicinal plant, Amomum nilgiricum belonging to Zingiberaceae family.…”

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confidence: 99%

GC–MS analysis of phytoconstituents from Amomum nilgiricum and molecular docking interactions of bioactive serverogenin acetate with target proteins

Konappa

Udayashankar

Krishnamurthy

et al. 2020

Sci Rep

138

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Amomum nilgiricum is one of the plant species reported from Western Ghats of India, belonging to the family Zingiberaceae, with ethno-botanical values, and is well-known for their ethno medicinal applications. In the present investigation, ethyl acetate and methanol extracts of A. nilgiricum were analyzed by Fourier transform infrared spectrometer (FTIR) and gas chromatography-mass spectrometry (GC–MS) to identify the important functional groups and phytochemical constituents. The FTIR spectra revealed the occurrence of functional characteristic peaks of aromatic amines, carboxylic acids, ketones, phenols and alkyl halides group from leaf and rhizome extracts. The GC–MS analysis of ethyl acetate and methanol extracts from leaves, and methanol extract from rhizomes of A. nilgiricum detected the presence of 25 phytochemical compounds. Further, the leaf and rhizome extracts of A. nilgiricum showed remarkable antibacterial and antifungal activities at 100 mg/mL. The results of DPPH and ferric reducing antioxidant power assay recorded maximum antioxidant activity in A. nilgiricum methanolic leaf extract. While, ethyl acetate leaf extract exhibited maximum α-amylase inhibition activity, followed by methanolic leaf extract exhibiting aldose reductase inhibition. Subsequently, these 25 identified compounds were analyzed for their bioactivity through in silico molecular docking studies. Results revealed that among the phytochemical compounds identified, serverogenin acetate might have maximum antibacterial, antifungal, antiviral, antioxidant and antidiabetic properties followed by 2,4-dimethyl-1,3-dioxane and (1,3-13C2)propanedioic acid. To our best knowledge, this is the first description on the phytochemical constituents of the leaves and rhizomes of A. nilgiricum, which show pharmacological significance, as there has been no literature available yet on GC–MS and phytochemical studies of this plant species. The in silico molecular docking of serverogenin acetate was also performed to confirm its broad spectrum activities based on the binding interactions with the antibacterial, antifungal, antiviral, antioxidant and antidiabetic target proteins. The results of the present study will create a way for the invention of herbal medicines for several ailments by using A. nilgiricum plants, which may lead to the development of novel drugs.

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Antioxidant, Biomolecule Oxidation Protective Activities of Nardostachys jatamansi DC and Its Phytochemical Analysis by RP-HPLC and GC-MS

Cited by 57 publications

References 57 publications

Spectrophotometric Determination of Cobalt(II) and Lead(II) Using (1,5-Dimethyl-2-Phenyl-4-((2,3,4-Trihydroxy Phenyl) Diazenyl)-1H-Pyrazol-3(2H)-One) as Organic Reagent: Using It as Antimicrobial and Antioxidants

Spectrophotometric Determination of Cobalt(II) and Lead(II) Using (1,5-Dimethyl-2-Phenyl-4-((2,3,4-Trihydroxy Phenyl) Diazenyl)-1H-Pyrazol-3(2H)-One) as Organic Reagent: Using It as Antimicrobial and Antioxidants

Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulis

GC–MS analysis of phytoconstituents from Amomum nilgiricum and molecular docking interactions of bioactive serverogenin acetate with target proteins

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