Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulis

Abstract

Herbal medicines are known to mitigate radical induced cell damage. Hence identification and scientific validation of herbal medicines contribute to better use in Ayurvedic/Unani research. In the present study, we investigated antioxidant and anti-apoptotic properties of Convolvulus pluricaulis (C. pluricaulis). C. pluricaulis exhibited antioxidant potential evident by free radical scavenging activities. C. pluricaulis pretreatment inhibited H2O2 induced macromolecule damage such as plasmid DNA damage and AAPH induced oxidation of bovine serum albumin and lipid peroxidation of rat hepatic tissues. Further to identify the neuroprotective properties of C. pluricaulis, SHSY5Y cells were treated with H2O2 with or without pretreatment of C. pluricaulis. The C. pluricaulis pretreatment at 50 μg/ml dose exhibited 50% cell survival against 100 μM H2O2 challenge for 24 h and it also decreased the lactate dehydrogenase leakage. Further C. pluricaulis pretreatment restored and regulated the antioxidant and apoptosis markers such as SOD, CAT, p53, and caspase-3 and inhibited, reactive oxygen species generation and depolarization of the mitochondrial membrane. C. pluricaulis possess a high content of flavonoids and polyphenols and GC-MS and FTIR analysis showed a wide variety of compounds which may contribute to the observed effects.

Role of Lipid Peroxidation Process in Neurodegenerative Disorders

“…However, the limited agents are tested in the management of dopaminergic neurodegeneration. Hence, the more extensive studies are required for the treatment of PD with direct and specific anti-lipid peroxidative drugs or combination with subsequent cell signal arresting molecules [70,71].…”

Convolvulus plant—A comprehensive review from phytochemical composition to pharmacy

“…The qualitative analysis of C. prostratus reveals the presence of alkaloids, flavonoids, steroids, glycosides, fatty acids, terpenoids, tannins, proteins, amino acids, saponines, phenolic compounds, and resin (Balaji, Chek Hean, Ravichandran, & Sikarwar, ; Kaushik, Jain, & Majumder, ; Rachitha et al, ; Shalavadi, Chandrashekhar, & Muchchandi, ; Sharma, Bhatnagar, & Kulkarni, ; Sharma, Verma, & Prasad, ). Compounds, such as microphyllic acid, kaempferol, kaempferol‐3‐glucoside, 3,4 dihydroxycinnamic acid, scopoletin, convolamine, convolidine, sitosterols (Amin, Sharma, Vyas, Prajapati, & Dhiman, ; Garg et al, ; Kulkarni, Girish, & Kumar, ), 2‐butanone, pentanoic acid, cinnamic acid, silane, decanoic acid, 2‐pentanol, ascorbic acid, 10‐bromodecanoic acid, tridecane, phthalic acid, eicosane, octatriacontyl pentafluoropropionate, 1‐octadecanesulphonyl chloride, squalene, pyrimidine, heneicosane, 1,2‐benzenedicarboxylic acid, cyclononasiloxane, octadecamethyl, nonacosane, sulphurous acid pentadecyl 2‐propyl ester, straight chain hydrocarbons (C22–C33), fatty acids (C14–C28), fatty alcohols (C24–C32), vitamin E, and cyclononasiloxane, are found in this plant (Rachitha et al, ; Srivastava & Deshpande, ).…”

“…The pharmacological potential of these plants comes from their chemical constituents with biological activities. Pharmacological activities were reported on some Convolvulus species such as the use of C. pilosellifolius as an antiulcerogenic (Atta, Mohamed, Nasr, & Mouneir, ; Awaad et al, ), C. arvensis and C. pilosellifolius inhibited tumor growth (Al‐Enazi, ; Meng et al, ), and C. prostratus against Alzheimer's disease (Kizhakke et al, ) and treat stress‐induced neurodegeneration (Rachitha et al, ), while C. prostratus for preventing aluminum‐induced neurotoxicity (Bihaqi et al, ). Indeed, the significance of Convolvulus plants is not only for medicinal applications but also for ancient and religious significance because it reminded people of certain aspects of their gods and goddesses (Austin, ).…”

Convolvulus aucheri’nin In vitro Antioksidan Özelliği ve LPS-aktif NSCLC Hücrelerinde NO Engelleyici Potansiyeli

“…With respect to recent researches, this genus is represented with 35 species in Turkey (Güner et al, 2012). Extracts of various members of Convolvulus have been demonstrated to have antioxidant, anticancer and antinociceptive activities (Rachitha et al, 2018;Atta and El-Sooud, 2004;Sadeghi-Aliabadi et al, 2008).…”