Antioxidant, Antimutagenic, and Anticarcinogenic Effects ofPapaver rhoeasL. Extract onSaccharomyces cerevisiae

Abstract

The aim of this work was to analyze the antioxidant and antimutagenic/anticarcinogenic capacity of Papaver rhoeas L. water extract against standard mutagen/carcinogen methyl methanesulfonate (MMS) and radiomimetic zeocin (Zeo) on a test system Saccharomyces cerevisiae. The following assays were used: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, quantitative determination of superoxide anion (antireactive oxygen species [antiROS test]), DNA topology assay, D7ts1 test--for antimutagenic--and Ty1 transposition test--for anticarcinogenic effects. Strong pro-oxidative capacity of Zeo was shown to correlate with its well-expressed mutagenic and carcinogenic properties. The mutagenic and carcinogenic effects of MMS were also confirmed. Our data concerning the antioxidant activity of P. rhoeas L. extract revealed that concentration corresponding to IC(50) in the DPPH assay possessed the highest antioxidant activity in the antiROS biological assay. It was also observed that a concentration with 50% scavenging activity expressed the most pronounced antimutagenic properties decreasing Zeo-induced gene conversion twofold, reverse mutation fivefold, and total aberrations fourfold. The same concentration possessed well-expressed anticarcinogenic properties measured as reduction of MMS-induced Ty1 transposition rate fivefold and fourfold when Zeo was used as an inductor. Based on the well-expressed antioxidant, antimutagenic, and anticarcinogenic properties obtained in this work, the P. rhoeas L. extract could be recommended for further investigations and possible use as a food additive.

Identification and metabolite profiling of alkaloids in aerial parts of Papaver rhoeas by liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry

“…For further identification of the alkaloids, the LC-QTOF base peak chromatogram collected in information-dependent acquisition scan mode was processed to search and screen alkaloid metabolites by exact masses against an in-house library and databases such as Chemspider, Metlin, and MS bank. The proposed identity of the alkaloid was compared F I G U R E 3 Representative metabolite intensities as determined by LC-QTOF analysis for samples at different growth stages (30,60, and 90 cultivating days): DL-demethylcoclaurine (1), coclaurine (2), tetrahydropapaverine (3), (R/S)-reticuline (4 and 6), corytuberine (5), L-tetrahydropalmatine (7), scoulerine (8), L-tetrahydropalmatine (9), tetrahydroberberine (10), berberine (11), stylopine (12), dihydrosanguinarine (13), sanguinarine (14), protopine (15), allocryptopine (16), and rhoeadine (42). The ratio is the peak intensity of a metabolite in a sample that was divided by the peak intensity of a blank sample and expressed in a logarithmic scale.…”

“…It has been used in folk medicine for the treatment of inflammation, cough, diarrhea, respiratory problems, asthma, insomnia, and pain, and it can also be consumed as food [1][2][3][4][5]. Various phytochemical components can be found in corn poppy, such as alkaloids [2,[6][7][8], anthocyanins [9], flavonoids [10], and essential oils [11]. P. rhoeas can be cultivated in regions with a temperate climate.…”

Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs

“…Papaver spp. has various functional benefits that are widely used to treat various issues, such as dental problems [ 10 ], neuronal disorders [ 11 ], cough [ 12 ], pain relief [ 13 ], microbial infections [ 14 ], and cancer [ 15 ]. Some species’ seeds, pedicles, and petals are used in salads or sorbets [ 16 ].…”

Microwave-Assisted Extraction of Polyphenolics from Some Selected Medicinal Herbs Grown in Turkey

“…platydiscus Cullen has been demonstrated [28]. Numerous studies have indicated that Papaver fugax Poiret var.…”