Antioxidant, antimicrobial and antiproliferative activities of Anthemis palestina essential oil

Abstract

BackgroundAnthemis palestina (Asteraceae) extends across the Mediterranean region, southwest Asia and eastern Africa. Although traditionally used for several applications, in vitro investigation of biological functions associated with Anthemis palestina essential oil had never been reported.MethodsThe air-dried flowers of Anthemis palestina were subjected to hydrodistillation to yield the oil. The antioxidant activity of the hydrodistilled oil was characterized using various in vitro model systems such as DPPH, ferric-reducing antioxidant power and hydroxyl radical scavenging activity. Antibacterial activity was tested against six bacterial species, representing both Gram positive and Gram negative bacteria. Antifungal activity was evaluated using three Candida species. The minimum inhibitory concentration (MIC) for each examined microorganism was determined using the microdilution method. The oil’s antiproliferative effects against eight human cancer cell lines were also studied and the lethal doses that resulted in 50% reduction of cell viability (LD50) were determined.ResultsThe results indicate that the essential oil of Anthemis palestina exhibited substantial antioxidant activities as demonstrated with DPPH, ferric reducing antioxidant power, and hydroxyl radical scavenging activity. In addition, a broad-spectrum antibacterial activity of the oil was revealed with better susceptibility of Gram positive bacteria towards the oil. The MIC values ranged between 6–75 μg/ml. Besides, the oil demonstrated a moderate inhibitory effect on the three Candida species examined; with MIC values ranging between 48–95 μg/ml. Potent cytotoxic activities, especially against HeLa cell line; with LD50 of 32 μg/ml, BJAB cell line; with LD50 of 57 μg/ml, and Caco-2 cell line; with LD50 of 61 μg/ml, were observed.ConclusionThe results obtained indicate high potential of Anthemis palestina essential oil as bioactive oil, for nutraceutical and medical applications, possessing antioxidant, antimicrobial and antiproliferative activities.

Phytochemical, antimicrobial, and antioxidant activities of different citrus juice concentrates

“…This is especially important in light of the increasing incidence of candiadiasis infections as well as the emergence of resistant Candida species to existing antifungal drugs (Bardaweel et al. ).…”

“…Candida albicans growth was however significantly inhibited by the juice concentrates. This is especially important in light of the increasing incidence of candiadiasis infections as well as the emergence of resistant Candida species to existing antifungal drugs (Bardaweel et al 2014). Table 6 shows the inhibitory and bacteriocidal concentrations of the citrus juice concentrates against some bacterial strains.…”

Genoprotective Effects of Essential Oil Compounds Against Oxidative and Methylated DNA Damage in Human Colon Cancer Cells

“…Individual EO usually contain only a few major EOC, for instance linalool is a major compound in coriander oil and basil (60% to 70% of all EOC) while eugenol makes up 80% of EOC in clove oil (Burt, ). EOC are highly bioactive aromatic components of plants and exert a wide range of proposed biological activities including antioxidative, antimicrobial, and anti‐inflammatory properties as well as antigenotoxicity and anticarcinogenicity (Ait‐Ouazzou et al., ; Bardaweel, Tawaha, & Hudaib, ; Bidinotto et al., ; Dörsam, Wu, Efferth, Kaina, & Fahrer, ; Teixeira et al., ). EO and EOC have been utilized as antimicrobials in cosmetics and toiletries (Muyima, Zulu, Bhengu, & Popplewell, ), foods (Tongnuanchan & Benjakul, ), and animal feed (Wallace et al., ), and are also used in alternative and complementary therapy (Ali et al., ; Wagner et al., ).…”

Fatty Acids Analysis, Antioxidant and Biological Activity of Fixed Oil ofAnnona muricataL. Seeds

“…where is the percentage of gastrointestinal transit in the vehicle treated group and is the percent of gastrointestinal transit in sample treated group. [24]. The following cell lines were studied to ensure the coverage of a wide range of cancer types: human ductal breast epithelial tumor T47D, human breast adenocarcinoma MCF-7, human epithelial carcinoma HeLa, human epithelial colorectal adenocarcinoma Caco-2, human colorectal adenocarcinoma cell line HRT, and human kidney carcinoma cell line A498.…”

“…The optimal plating densities were determined according to the cells growth profiles. To assess the antiproliferative activity, MTT assay was carried out using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazoliumbromide (MTT, Sigma-Aldrich, USA) assay, as previously described [24]. The concentration that inhibits the proliferation of oil-treated cells by 50% relative to the control (untreated cells) was used to evaluate the cytotoxic activity of the oil.…”