Antioxidant, antimicrobial and antifeedant activity of phenolic compounds accumulated in Hyoscyamus muticus L.

Abstract

Hyoscyamus muticus is an endangered desert plant spread in the Arabian Peninsula and the Middle East deserts. The methanol extract of the aerial parts of H. muticus grown in the arid zones in the Northern regions of Saudi Arabia were subjected to primary phytochemical analysis which revealed the presence of phenolic compounds, flavonoids, tannins and sterols, the gas chromatography-mass spectrometry (GC-MS) analysis of the methanolic extract exhibited different types of phenolic compounds, including ferulic acid, 4-hydroxy-cinamic acid-ester, methyl salicylate and methyl ferulate. The accumulation of phenolic compounds supports the antioxidative properties of the plant against oxidative stress. The antioxidant testing showed that the methanolic extract of H. muticus has a noticeable antioxidant activity with an IC 50 of 8.1±0.65 mg/ml and an EC 50 of 12.74±1.12 mg/ml. The antimicrobial investigation on 11 microbial strains revealed that the methanolic extract of H. muticus areal parts showed average or weak antibacterial activity against gram-positive bacteria, weak antibacterial activity against gram-negative bacteria and no antifungal activity. Moreover, the investigations exhibited the presence of an antifeedant potential on the methanol extract of H. muticus on the 4 th instar larvae of Spodoptera littoralis.

Effect of silver nanoparticles on tropane alkaloid production of transgenic hairy root cultures of Hyoscyamus muticus L. and their antimicrobial activity

“…However, it did not exhibit any activity against A. niger but was effective against several other fungi. In contrast, Elsharkawy et al 49 found that the methanolic extract of H. muticus aerial parts was successful in reducing the growth of S. aureus and Bacillus cereus . They also observed an inhibitory effect on E. coli , K. pneumoniae , and P. aeruginosa .…”

Identification of Compounds of Crocus sativus by GC-MS and HPLC/UV-ESI-MS and Evaluation of Their Antioxidant, Antimicrobial, Anticoagulant, and Antidiabetic Properties

“…Moreover, the difference between the antioxidant activity of the extracts studied in this work and those from China could be due to the difference in their polyphenol, flavonoid, and tannin contents and experimental conditions (plant, standard, etc.). Indeed, many studies have previously mentioned that phenolic compounds have remarkable antioxidant properties in both in vitro and in vivo systems [34][35][36].…”

Phenolic content, antibacterial, antioxidant, and toxicological investigations of Erodium guttatum (Geraniaceae) collected from the Northeast of Morocco

“…According to the phytochemical analysis, methanol extract has the higher phenolic content, which demonstrates its effectiveness against the tested bacteria compared to aqueous extract. In fact, previous studies have been demonstrated the correlation between the antibacterial activity of plant extracts and its phenolic content (Rodríguez Vaquero et al 2010;Tomás-Menor et al 2013;Elsharkawy et al 2018).…”