Antioxidant, antihemolysis, and retinoprotective potentials of bioactive lipidic compounds from wild shrimp (Litopenaeus stylirostris) muscle

Abstract

The oxidative stress damage on cells is an example of roles in the pathogenesis of different degenerative diseases and the search of compounds that can slow this oxidation is continuous. The aim of this work was to obtain bioactive fractions from wild shrimp (Litopenaeus stylirostris) muscle, in order to evaluate their protective capacity and chemo-structurally characterize them. ABTS and DPPH, and FRAP assays suggested that bioactive fractions possess free radical-scavenging capacity, and reducing power, respectively. An inhibitory effect observed on AAPH-induced hemolysis and results from an H 2 O 2-derived radicals-scavenging assay (retinoprotective) suggest that these fractions can exert their protective activity in human cells. UV-Vis, fluorescence, 13 C-, 1 H-NMR, and ESI-MS studies performed on the most bioactive fraction, suggests that their main components are eicosapentaenoic acid, dioctyl phthalate, and a possibly novel indolocarbazole alkaloid derivative. These results suggest that these compounds are good candidates to further investigations as possible chemoprotective agents. Potencial antioxidante, antihemólisis y retinoprotectorr de compuestos lipídicos bioactivos provenientes del músculo del camarón salvaje (Litopenaeus stylirostris) RESUMEN El daño por estrés oxidativo sufrido por las células es un ejemplo de roles en la patogénesis de diferentes enfermedades degenerativas, por lo que la búsqueda de compuestos que retrasen dicha oxidación es continua. Este trabajo tuvo por objetivo obtener fracciones bioactivas del músculo de camarón salvaje (Litopenaeus stylirostris), con la finalidad de evaluar su capacidad protectora y de realizar su caracterización quimioestructural. Los ensayos ABTS-DPPH y FRAP indicaron que las fracciones bioactivas poseen capacidad de eliminación de radicales libres y poder reductor, respectivamente. La hemólisis inducida por AAPH permite observar un efecto inhibitorio. Los resultados procedentes de un ensayo de eliminación de radicales derivados de H 2 O 2 (retinoprotector), sugieren que estas fracciones pueden ejercer actividad protectora en células humanas. Los estudios de UV-Vis, fluorescencia, 13 C-, 1 H-NMR y ESI-MS aplicados a la fracción más bioactiva mostraron que sus componentes principales son ácido eicosapentaenoico, ftalato de dioctilo y un derivado de alcaloide de indolocarbazol posiblemente nuevo. Tales resultados insinúan que estos compuestos son buenos candidatos para que futuras investigaciones los evalúen como posibles agentes quimioprotectores.

Evaluating the effects of different processing methods on the nutritional composition of shrimp and the antioxidant activity of shrimp powder

“…A previous study on the antioxidant and antihemolytic activities of the wild shrimp muscle fractions showed that one fraction produced significantly higher μM Trolox equivalent (TE)/g values of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS; 21.4 ± 2.4), DPPH (25.2 ± 1.5), and ferric reducing power (FRAP;166.8 ± 7.2) ( García-Romo et al, 2020 ). A separate study reported that the peel of fresh shrimp and the peel of fresh and ground shrimp of Litopenaeus vannamai was possessed greater antioxidant efficacy ( Ratanasiriwat and Pienchob, 2018 ).…”

Encapsulation with HDPAF-WP of the hexane fraction of sea grape (Coccoloba uvifera L.) leaf extract by electrospraying

“…However, its prevention turns out to be more convenient since it is less expensive [3]. In this way, the identification of new sources of compounds with high biological value (HBVC) and some biological activities to prevent the CDDs is necessary [4,5]. HBVC are naturally found in plants, have the advantage of being natural, safe for consumption, low cost, and have preventive and therapeutic activities against CDDs [3].…”

Octopus vulgaris ink extracts exhibit antioxidant, antimutagenic, cytoprotective, antiproliferative, and proapoptotic effects in selected human cancer cell lines

“…Besides, antimutagenic compounds exhibit a chemopreventive effect against mutagen‐induced DNA and promote a slow cancer initiation. Furthermore, the antiproliferative activity is directly related to the development of this disease, representing a tool in the search for new candidate compounds that may either prevent or slow down uncontrolled cancer cell division by interfering with the cell cycle (García‐Romo et al., 2020; López‐Saiz, Suárez‐Jiménez, Plascencia‐Jatomea, & Burgos‐Hernández, 2013).…”