Antioxidant, anticlastogenic and radioprotective effect of Coleus aromaticus on Chinese hamster fibroblast cells (V79) exposed to gamma radiation

Abstract

Coleus aromaticus (Benth, Family: Laminaceae), Indian Oregano native to India and Mediterranean, is well known for its medicinal properties. A preliminary study was undertaken to elucidate in vitro free radical scavenging potential and inhibition of lipid peroxidation by C.aromaticus hydroalcoholic extract (CAE). Anti-clastogenic and radioprotective potential of CAE were studied using micronucleus assay after irradiating Chinese hamster fibroblast (V79) cells. CAE at 10, 20, 40, 60, 80, 100 and 120 mug/ml resulted in a dose-dependent increase in radical scavenging ability against various free radicals viz., 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide anion (O(2)(*-)), hydroxyl (OH(*)) and nitric oxide (NO(*)) generated in vitro. A maximum scavenging potential was noticed at 100 mug/ml and a saturation point was reached thereafter with the increasing doses of CAE. The free radical scavenging potential of the extract was in the order of DPPH > ABTS > Superoxide > Hydroxyl > Nitric oxide. CAE also exhibited a moderate inhibition of lipid peroxidation in vitro, with a maximum inhibition at 60 mug/ml (33%), attaining saturation at higher doses. The extract also rendered protection against radiation induced DNA damage, as evidenced by the significant (P < 0.05) decrease in the percentage of radiation-induced micronucleated cells (MN) and frequency of micronuclei (total). A maximum anticlastogneic effect/ radioprotection was noticed at a very low concentration i.e., 5 mug/ml of CAE, treated 1 h prior to 2 Gy of gamma radiation. A significant (P < 0.0001) anticlastogenic/radioprotective effect was also observed when the cells were treated with an optimum dose of CAE (5 mug/ml) 1 h prior to 0.5, 1, 2 and 4 Gy of gamma radiation compared with the respective radiation control groups. Overall, our results established an efficient antioxidant, anticlastogenic and radioprotective potential of CAE, which may be of great pharmacological importance.

Radiomodulatory role of Rutin and Quercetin in Swiss Albino mice exposed to the whole body gamma radiation

“…[378] It may be therefore logical to expect that extracts and bioactive compounds with antioxidant potential derived from plants may render radioprotection to normal tissues as evidenced from earlier reports. [79101112131415]…”

Plant Extracts and Plant-Derived Compounds: Promising Players in Countermeasure Strategy Against Radiological Exposure: A Review

“…(Family: Laminaceae), is an important medicinal plant used widely to treated a number of illness (Lans et al, 2007;Pritima and Pandian, 2007) and has been reported to protect liver and other disorders in rats (Choudhary, 2009;Vijayavel et al, 2013). The antioxidant potential of this plant was investigated in various standard in vitro free radical generating model systems that included DPPH, 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), O 2 •-, ˙OH and nitric oxide (NO) (Rao et al, 2006). Different concentrations (10-120 μg/ml) of hydroalcoholic extract of C aromaticus (CAE) showed concentration-dependent increase in radical scavenging ability against various free radicals.…”

Chemopreventive Activity of Mediterranean Medicinal Plants

“…In vitro studies with cancer cell lines, showed that oregano extract protect against oxidative stress and DNA damage from radiation (Rao et al, 2006;Bakkali et al, 2006). Additionally, oregano extract was found to have strong antioxidant activity, through the capture free radicals, suppression of fat oxidation, inhibition of NOS and protection of DNA from H 2 O 2 -induced oxidative damage (Zheng & Wang, 2001;Aherne et al, 2007;Tsai et al, 2007).…”