Antioxidant, Anti-Lipoxygenase and Cytotoxic Activity of Leptadenia pyrotechnica (Forssk.) Decne Polyphenolic Constituents

Abstract

Leptadenia pyrotechnica Forssk is a traditional medicinal herb used for treatment of inflammatory diseases and cancer. In this research, the aqueous ethanolic crude extract of Leptadenia pyrotechnica aerial parts, along with its ethyl acetate, n-butanol and water partitioning fractions were evaluated for their antioxidant capacity, polyphenolic content, anti-inflammatory and anti-cancer properties. The total antioxidant capacity was estimated by the FRAP, DPPH, ABTS and β-carotene bleaching assays.The ethyl acetate fraction exhibited the highest polyphenolic content (252.27 mg gallic acid/g) and the best antioxidant activity (1.2, 0.57, 0.45 mmol ascorbic acid equivalent/g in the FRAP, ABTS and DPPH assays, respectively). Furthermore, the same extract showed appreciable anti-inflammatory via lipoxygenase (LOX) inhibitory activity (IC50 = 1.41 µg/mL). Moreover, the ethyl acetate fraction also showed the strongest cytotoxic effect (IC50 = 43.16 µg/mL) against MCF-7 human breast cancer cell line. These results suggest that this plant may be considered an interesting source of compounds with antioxidant, anti-inflammatory and anti-cancer properties for therapeutic, nutraceutical and functional food applications.

Biological activity of fluidized bed ethanol extracts from several edible mushrooms

“…5). Compared with extracts of L. giganteus, the EC 50 values obtained from the 2 wild edible mushrooms (P. ostreatus PQMZ91109 and PBS281009) were different, indicating the presence of a larger quantity of biomolecules with antioxidant effects for inhibition of the peroxyl radical action (15). By contrast, the EC 50 values of extracts were similar to values achieved using simple methanol extracts from fruit bodies of Lactarius piperatus (27), being approximately 45% lower than TBHQ values.…”

“…The inhibition assay for xanthine oxidase was performed as described by Sahgal et al (14), and the inhibition assay for lipoxygenase was performed following the method of Khasawneh et al (15).…”

ETHNOBOTANICAL AND BIOLOGICAL ACTIVITIES OF Leptadenia pyrotechnica (Forssk.) Decne.: A REVIEW

“…It is planted in the month of September (Moustafa et al, 2007). Recently, it is also cultivated in forests, farms and on the roadsides (Khasawneh et al, 2011).…”

Phytochemical Profiling, Antibacterial Potential and GC-MS Analysis of Methanol Leaf Extract of Enicostemma axillare

“…The phytol is the compound which inactivates the protein and enzymes present in the microorganisms. In addition, the compound has no remarkable toxicity and possess high stability (Alqasoumi et al, 2012;Khasawneh et al, 2011;Dagla et al, 2012). Thus, it is evident from the present findings that phytol along with other phytoconstituents plays a major role in the inhibition of the growth of drug resistant E. coli.…”