Antioxidant, anti-inflammatory and hepatoprotective effects of olive fruit pulp extract: <i>in vivo</i> and <i>in vitro</i> study

Almatroodi, Saleh A.; Almatroudi, Ahmad; Anwar, Shehwaz; Babiker, Ali Yousif; Khan, Amjad Ali; Alsahli, Mohammed A.; Rahmani, Arshad Husain

doi:10.1080/16583655.2020.1848761

Cited by 14 publications

(10 citation statements)

References 42 publications

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“…The result agrees with Almatroodi 44,45 . They mentioned that the levels of antioxidant enzymes were considerably reduced in CCl 4 -induced animals relative to the control.…”

Section: Discussionsupporting

confidence: 88%

Synergistic effect of Rosemary and Lemon extractions on some physiological and biochemical parameters of CCl4-Stressed male rats

Al-Mushhadani

Al-Hayali

Mostafa

2023

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This study was carried out to examine the preventive impact of marine extracts of rosemary and lemon individually or together in adult-male rats with liver injury induced by carbon tetrachloride. The extracts were prepared and tested on 40 male rats distributed into groups by collecting blood samples and conducting some hematological and biochemical parameters. CCl4-induced significant increases in the weight of the liver and heart, while rosemary and lemon extracts alone were not able to restore the liver weight, but the effect was in the synergy. As well, the extracts alone or in combination had a significant impact on reducing heart weight. Additionally, CCl4 caused a substantial reduction in RBC, Hb, PCV, MCV, MCHC and lymphocytes, whereas WBC, monocyte and granulocyte increased. While extracts exhibited an enhancement in these parameters, the best effect was when the two extracts were used together. The biochemical parameters indicated high significance in glucose, AST, ALT and triglycerides; however, total protein, albumin, cholesterol and T-AOC decreased compared to the control group. Both rosemary and lemon worked to restore and remove the oxidative effect. Keywords: Rosemary(A plant that grows in mountainous regions), Lemon, Carbon tetrachloride, Stress, Rats.

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“…The result agrees with Almatroodi 44,45 . They mentioned that the levels of antioxidant enzymes were considerably reduced in CCl 4 -induced animals relative to the control.…”

Section: Discussionsupporting

confidence: 88%

Synergistic effect of Rosemary and Lemon extractions on some physiological and biochemical parameters of CCl4-Stressed male rats

Al-Mushhadani

Al-Hayali

Mostafa

2023

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“…The antioxidant activity was further confirmed on the basis of scavenging 1,1 difenyl-2-picryl-hydrazyl (DPPH) as described in prior articles [ 19 , 22 , 23 ]. Briefly, 2.5 mL of increasing concentration of methanolic extracts (50, 100, 200, 300, 400, 500, and 600 μg/mL) was mixed with DPPH (1 mL, 0.3 mM; prepared in analytical grade methanol).…”

Section: Methodsmentioning

confidence: 98%

Role of Ajwa Date Fruit Pulp and Seed in the Management of Diseases through In Vitro and In Silico Analysis

Anwar

Raut

Alsahli

et al. 2022

Biology

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This study investigated the health-promoting activities of methanolic extracts of Ajwa date seed and fruit pulp extracts through in vitro studies. These studies confirmed potential antioxidant, anti-hemolytic, anti-proteolytic, and anti-bacterial activities associated with Ajwa dates. The EC50 values of fruit pulp and seed extracts in methanol were reported to be 1580.35 ± 0.37 and 1272.68 ± 0.27 µg/mL, respectively, in the DPPH test. The maximum percentage of hydrogen peroxide-reducing activity was 71.3 and 65.38% for both extracts at 600 µg/mL. Fruit pulp and seed extracts inhibited heat-induced BSA denaturation by 68.11 and 60.308%, heat-induced hemolysis by 63.84% and 58.10%, and hypersalinity-induced hemolysis by 61.71% and 57.27%, and showed the maximum anti-proteinase potential of 56.8 and 51.31% at 600 μg/mL, respectively. Seed and fruit pulp inhibited heat-induced egg albumin denaturation at the same concentration by 44.31 and 50.84%, respectively. Ajwa seed showed minimum browning intensity by 63.2%, percent aggregation index by 64.2%, and amyloid structure by 63.8% at 600 μg/mL. At 100 mg/mL, Ajwa seed extract exhibited good antibacterial activity. Molecular docking analysis showed that ten active constituents of Ajwa seeds bind with the critical antioxidant enzymes, catalase (1DGH) and superoxide dismutase (5YTU). The functional residues involved in such interactions include Arg72, Ala357, and Leu144 in 1DGH, and Gly37, Pro13, and Asp11 in 5YTU. Hence, Ajwa dates can be used to develop a suitable alternative therapy in various diseases, including diabetes and possibly COVID-19-associated complications.

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“…Ibuprofen was serially diluted in phosphate buffer (pH 6.4) for the test of inhibition of heat-induced denaturation inhibition. Diclofenac sodium, on the other hand, was prepared by diluting in Tris HCl buffer, pH 7.4, for antiproteinase activity testing [17]. Studies of active drug/excipient compatibility represent an important phase in the pre-formulation stage of the development of all dosage forms.…”

Section: Reference Drug Preparationmentioning

confidence: 99%

“…This capacity of myrrh was conducted utilizing a test involving DPPH (1,1 diphenyl-2picryl-hydrazyl) radical according to our previously published article [17]. Myrrh extract (50,75,100,200, 300, 400, 500, and 600 µg/mL) and 1 mL of 0.3 mM methanolic DPPH solution were mixed in different vials, and solutions were placed in a dark place for 30 min.…”

Section: Dpph Assaymentioning

confidence: 99%

Therapeutic Potential of Myrrh, a Natural Resin, in Health Management through Modulation of Oxidative Stress, Inflammation, and Advanced Glycation End Products Formation Using In Vitro and In Silico Analysis

et al. 2022

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Oxidative stress, hyper-inflammatory responses, and protein glycation are the chief contributing factors in the pathogenesis of several diseases. This study aimed to explore the therapeutic role of myrrh in health management through in vitro and in silico studies. Antioxidant potential, anti-inflammatory potential, antiglycation, and advanced glycation end products (AGEs) formation inhibition activities were determined by various in vitro assays. Molecular docking was performed to predict the non-covalent binding of macromolecules (receptor) and a small molecule (ligand). Myrrh extract contained significant antioxidant activity as reflected by FRAP value (16.12 µg ascorbic acid/100 mg dry weight), the maximum percentage of DPPH scavenging (57.71%), and maximum hydrogen peroxide reducing activity (58.71%) at a concentration of 600 µg/mL. Further, the extract exhibited maximum protection from bovine serum albumin (BSA) denaturation inhibition (53.47%), anti-proteinase action (43.517%), and egg albumin denaturation inhibition (44.95%) at a dose of 600 µg/mL concentration. Myrrh is used in pharmacy as an antiseptic, anti-inflammatory, antimicrobial, antifungal, and anti-venom remedy. This study aimed to explore the antioxidant, anti-inflammatory, antiarthritic, antiglycation, and advanced glycation end products (AGEs) formation inhibition ability of methanolic myrrh extract. It was found that myrrh has good antioxidant potential due to plenty of flavonoids and polyphenolic compounds, as reflected by results of the 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH) assay, FRAP (ferric reducing antioxidant power) test, and hydrogen peroxide reducing test. Furthermore, myrrh methanolic extract was found to be significantly effective against heat-induced albumin denaturation, and percent inhibition of denaturation increases with increased extract concentration. The presence of myrrh extract at a dose of 600 μg/mL decreased browning intensity (59.38%), percent aggregation index (59.88%), and percentage amyloid structure (56.13%). We used molecular docking tools to study the role of myrrh in oxidative stress (Catalase), antioxidant property (Superoxide dismutase), and antiviral property (spike protein S). The molecular docking analysis confirmed four phytoconstituents; 2,3-Furandione, Curzerene, delta-Elemene, and Furanoeudesma-1,3-Diene interact with catalase and superoxide dismutase. Curzerene and Furanoeudesma-1,3-Diene showed remarkable interaction with SARS-CoV-2 spike protein S. Our data suggest that myrrh resin extract can be used to develop a suitable alternative therapy for various diseases linked with oxidative stress, inflammation, glycation, and AGEs.

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Antioxidant, anti-inflammatory and hepatoprotective effects of olive fruit pulp extract: in vivo and in vitro study

Cited by 14 publications

References 42 publications

Synergistic effect of Rosemary and Lemon extractions on some physiological and biochemical parameters of CCl4-Stressed male rats

Synergistic effect of Rosemary and Lemon extractions on some physiological and biochemical parameters of CCl4-Stressed male rats

Role of Ajwa Date Fruit Pulp and Seed in the Management of Diseases through In Vitro and In Silico Analysis

Therapeutic Potential of Myrrh, a Natural Resin, in Health Management through Modulation of Oxidative Stress, Inflammation, and Advanced Glycation End Products Formation Using In Vitro and In Silico Analysis

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